Research Journal of Pharmacognosy and Phytochemistry

Abstract

In this paper, in vitro antioxidant and antimicrobial activities of ethyl acetate extract of Evodia lunu-ankenda (Gaertn) Merr. bark was determined by four methods Diphenylpicrylhydrazyl (DPPH), Nitric Oxide, Super oxide disumatase, Hydrogen peroxide (H₂O₂) and by agar disc diffusion methods. The crude ethyl acetate extract of Evodia lunuankenda bark inhibited the growth of both gram positive bacteria (Bacillus substilis, Staphylococcus aureus and micrococcus luters) and gram negative bacteria (Escharichia coil, Pseudomonas aeruglinosa and Salmonella typhlmurium) and also the crude ethyl acetate extract of Evodia lunuankenda bark inhibited the growth of fungi (candida albicans and Aspergillus niger). The ethyl acetate extract of Evodia lunuankenda bark showed dose dependent increase in reducing anti oxidant power that was comparable to standards. Antibacterial activity, highest concentration of ethyl acetate extract (5mg/ml) having good activity against gram positive and gram negative organisms as compared with the reference Ciprofloxacin. Antifungal activity, highest concentration of extract (5mg/ml) having good activity as compared with the reference Amphotericin-B. It can be concluded that the plant posses potent antioxidant and antimicrobial activity, responsible of the secondary metabolites like flavonoids, saponins, are likely observed for the plants.

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