Refine your search
Collections
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Tamizh Mani, T.
- Simultaneous Estimation of Ellagic Acid and Quercetin in Marketed Ayurvedic Formulations by HPTLC
Abstract Views :476 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacognosy, Bharathi College of Pharmacy, Bharathi Nagara, Maddur Tq., Mandya Dt., Karnataka -571422, IN
1 Department of Pharmacognosy, Bharathi College of Pharmacy, Bharathi Nagara, Maddur Tq., Mandya Dt., Karnataka -571422, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 9, No 4 (2017), Pagination: 223-227Abstract
A simple, rapid and reliable HPTLC method has been developed for simultaneous estimation of ellagic acid and quercetin in Ayurvedic formulations. Identification and quantification were performed on 20cm x 10cm, layer thickness 0.2mm, aluminium-backed silica gel 60 F254 HPTLC plates previously washed with methanol. Ethyl Acetate: Toluene: Formic Acid: Water (5:3:1:1, v/v), was used as a mobile phase. Calibration plots were established showing the dependence of response on the amount chromatographed. The linear regression analysis data for the calibration plots showed good linear relation with R2 = 0.998 and 0.9991 with respect to peak area for ellagic acid and quercetin respectively, in concentration range of 200-600ng/spot. The spots were scanned at λ = 225nm. The suitability of this HPTLC method for simultaneous estimation of the marker constituents was proved by validation in accordance with ICH Guidelines. The developed method has the advantage of being rapid and easy. Hence, it can be applied for routine quality control analysis of ellagic acid and quercetin in Ayurvedic formulations.Keywords
HPTLC, Validation, Ayurvedic Formulations, Ellagic Acid and Quercetin.
References
- Sheng-Teng Huang, Chen-Yu Wang, Rong-Chi Yang, Hsiao-Ting Wu, Su-Hui Yang, Yung-Chi Cheng, and Jong-Hwei S. Pang. Ellagic Acid, the Active Compound of Phyllanthus urinaria, Exerts In-Vivo Anti-Angiogenic Effect and Inhibits MMP-2 Activity. Evidence-Based Complementary and Alternative Medicine, 2011; 1-10.
- C. Gupta, A. Vikram, D. N. Tripathi, P. Ramarao and G. B. Jena. Antioxidant and Antimutagenic Effect of Quercetin against DEN Induced Hepatotoxicity in Rat. Phytother. Res, 2010; 24: 119– 128.
- Khan KH. Role of Emblica officinalis in medicine. Botany Research international, 2009; 2(4):218-28.
- Kokate CK, Purohit AP and Gokhale SB. Text book of Pharmacognosy, Jan 2007; 27:258-59.
- Pawar V, Lahorkar P and Ananth Narayana DB. Development of a RP-HPLC Method for analysis of Triphala curna and its Applicability to test variations in Triphala curna preparations. Ind J Pharm Sci, July-Aug 2009; 71(4):382-86.
- Jaganathan NS and Kannan K. HPTLC Method for estimation of Ellagic acid and Gallic acid in Triphala churnam formulations. Research J Phytochem, 2008; 2(1):1-9.
- Manimaran S, Subburaju T, Raja SS, Alok S, Vaibhav G, Chinnasamy K, Nanjan MJ and Suresh B. Comparative study of gallic acid content in marketed Siddha Formulations of Triphala Chooranam and tablets by HPTLC. Ind Drugs, Sep 2003; 40(9):532-34.
- International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text on Validation of Analytical Procedures Q2A, 1994.
- International Conference on Harmonization (ICH), Validation of Analytical Procedures: Methodology Q2B, 1996.
- International Conference on Harmonization (ICH), Validation of Analytical Procedures: Text and Methodology Q2 (R1), 2005..