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Gabhe, Satish Y.
- Comparative Antioxidant Activity of Three Garlic Varieties:An in vitro Study
Authors
1 C. U. Shah College of Pharmacy, S.N.D.T Women’s University, Santacruz (West), Mumbai-400049, IN
2 C. U. Shah College of Pharmacy, S.N.D.T Women’s University, Mumbai-400 049, IN
3 ICMR Advanced Centre of Reverse Pharmacology in Traditional Medicine, Kasturba Health Society, Vile Parle (West), Mumbai- 400 056, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 3, No 4 (2011), Pagination: 162-165Abstract
The present paper discuss the antioxidant activity of methanol extracts of commonly available three garlic varieties in India using various in vitro methods Viz, DPPH assay, total reducing power and TBARS assay. The results were analyzed statistically by regression method and IC50 values were calculated for all the extracts. The amounts of total phenolics and flavonoids were also determined for each extract of garlic. All the extracts exhibited good free radical scavenging activity. Antilipid peroxidation activity of all garlic varieties was comparable to standard antioxidant, ascorbic acid. Methanol extract of single bulb variety of garlic exhibited better antioxidant activity among all three garlic varieties. The study provides evidence for the beneficial role of garlic. The antioxidant activity of garlic extracts will effectively contribute to the prevention of various chronic conditions and also confirms its ancient traditional claims.
Keywords
Garlic, Allium sativum, Antioxidant, Free Radical Scavenging.
- Isolation and Quantitative Analysis of a Bioactive Polyphenol - Catechin in Anacardium occidentale Linn. (Leaves and Testa) by HPLC Analysis
Authors
1 C.U.Shah College of Pharmacy, S.N.D.T Women’s University, Mumbai-400049, IN
2 C. U. Shah College of Pharmacy, S.N.D.T. Women's University, Juhu Campus, Juhu Tara Road, Santacruz West, Mumbai-400 049, M.S., IN
3 ICMR Advanced Centre of Reverse Pharmacology in Traditional Medicine, Kasturba, Health Society, Vile Parle-(W), Mumbai-400 056, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 2, No 5 (2010), Pagination: 372-376Abstract
The present paper reports a method developed for isolation, characterization, structural elucidation and quantitative analysis of bioactive polyphenol - Catechin, from extracts of leaves and testa of Anacardium occidentale Linn (Cashew). Isolation of Catechin was carried out by Preparative TLC. The isolated Catechin was characterized by P-NMR, MS, IR spectroscopy and various chemical and chromatographic analysis. The method was established by using HPLC analysis and quantitation of isolated Catechin with marker Catechin. The purity of isolated Catechin was found to be 99.30% based upon the comparison of peak purity and peak areas of isolated Catechin and marker Catechin.
The HPLC separation system consisted of a C18 reversed-phase column, an isocratic elution system of methanol-water (9:1 v/v), and a UV-Visible detector with 254nm as the detection wavelength. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.2 μg and 0.6 μg respectively with an Rt value of 2.5 min.
The maximum amount of Catechin was found in aqueous extract of leaves (5.70%) and testa (13.65%).
Thus the developed HPLC method is rapid and simple technique for separation and determination of Catechin from extracts of A.occidentale leaves and testa. The method can be suitably applied for determination of Catechin from various plant extracts.