Research Journal of Pharmacognosy and Phytochemistry

D. S. Hugar ¹, Sanjeev Heroor ², Zakir Hussain ³

Affiliations
1 HKES’s College of Pharmacy, Gulbarga–585105, IN
2 Department of Pharmacognosy and Phytochemistry, HKES’s College of pharmacy Gulbarga-585105, IN
3 Luqman College of Pharmacy, Gulbarga-585102, IN

Abstract

Jatropha curcas Linn. (Euphorbiaceae) traditionally claimed to be used as tonic, tissue builder and stimulant, the present work was undertaken to prove scientifically its tonic property as immunomodulator (immunostimulant) on human neutrophils by some in-vitro models, viz.- Nitroblue tetrazolium (NBT) dye test, phagocytosis of candida albicans and chemotaxis assay at different concentrations - 10μg/ml, 20μg/ml, 40μg/ml, 100μg/ml and 1000μg/ml (undiluted extract) of aqueous and petroleum ether(40-60° c) extracts of seeds of the plant. The extracts at concentrations of 1000μg/ml (undiluted extract) and 100μg/ml showed 70- 80% of phagocytic and chemotactic activities when compared to normal and positive controls in the parameters studied. However, the activity was lower at concentrations 40μg/ml, 20μg/ml and 10μg/ml. Preliminary phytochemical investigation showed the presence of flavonoids, tannins, carbohydrates, saponins and amino acids in the extracts; hence these compounds might be responsible for immunomodulatory (immunostimulant) activity.

Keywords

Jatropha curcas, Immunomodulatory (Immunostimulant), NBT, Candida albicans, chemotaxis.

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Faheem I. Patwekar ¹, Sanjeev Heroor ², Mohsina F. Patwekar ³, Muhammad Asif ⁴

Affiliations
1 Dept. of Pharmacognosy, Luqman College of Pharmacy, Behind P and T Quarters, Old Jewargi Road, Gulbarga 585102, IN
2 HKES’s Society’s College of Pharmacy, Gulbarga, IN
3 Luqman College of Pharmacy, Gulbarga, IN
4 Allana College of Pharmacy, Pune, IN

Abstract

In the present study, the entire plant of Tephrosia procumbens Buch-Ham commonly known as Vempalli, Indigo sauvage (Fam-Fabaceae/ Leguminosae) was investigated for the phytochemical screening and antimicrobial property against certain microorganisms using disc diffusion method. The entire plant of Tephrosia procumbens were collected, shade dried at room temperature, pulverized and extracted with 95% ethanol in soxhlet extractor to get total ethanolic extract which is further fractionated with the solvents of different polarities. Total aqueous extract was also obtained by macerating the shade dried material with 3% of chloroform water I.P. All the extracts and fractions were subjected for Preliminary Phytochemical screening, which has shown the presence of Carbohydrates, Steroids and Flavonoids and evaluation of antimicrobial property by using disc diffusion method on various pathogenic forms of microorganisms including gram positive, gram negative and fungi. It was found that total ethanolic and total aqueous extract of entire plant of Tephrosia procumbens significantly inhibit the growth of microorganisms as compared to standard drugs Gentamycin and streptomycin (Hi-Media Lab. Mumbai).

Keywords

Tephrosia procumbens, Antimicrobial Activity.

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