Research Journal of Pharmacognosy and Phytochemistry

R. H. Gokani ¹, M. A. Rachchh ², S. K. Lahiri ³, D. D. Santani ⁴, M. B. Shah ³

Affiliations
1 “Shrikunj” 3-Archana Park, B/H Krishna Complex, University Road, Rajkot-360005, Gujarat, IN
2 Dept. of Pharmacognosy, S.J. Thakkar Pharmacy College, Rajkot, IN
3 Dept. of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad, IN
4 Dept. of Pharmacology L. M. College of Pharmacy, Ahmedabad, IN

Abstract

Root of Premna integrifolia known under common name Arni/Agnimantha which is an important constituent of well known formulation, Dashmularistha used for various ailments. In the present study methanolic extract of the ischolar_mains was evaluated for its in-vitro antioxidant activity using the anti radical, superoxide scavenging, anti lipid peroxidation, hydroxyl radical scavenging and nitric oxide scavenging, assays. The extract showed significant anti-oxidant activity in all the assays. The study scientifically demonstrated the use of P. integrifolia as a potential source of natural antioxidant.

Keywords

P. integrifolia, Verbanaceae, Anti-Oxidant Activity.

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U. D. Shah ¹, M. B. Shah ², A. K. Saluja ³

Affiliations
1 Department of Pharmacognosy, APMC College of Pharmaceutical Education Research, Himatnagar, IN
2 Dept. of Pharmacognosy, L. M. College of Pharmacy, Ahmedabad, IN
3 Dept. of Pharmacognosy, A. R. College Pharmacy, Vallabh Vidyanagar, IN

Abstract

Fresh stembark of Albizzia lebbeck was studied for macro and microscopical characters. Betulinic acid was isolated and identified by M. P., co-chromatography with reference standard and IR spectral characteristics. HPTLC method was developed for quantification of betulinic acid using precoated silica gel plates as a stationary phase, Toluene: Acetone: Acetic acid (100:1:0.1) as a mobile phase and anisaldehyde sulphuric acid as a spray reagent. The bark is rough as longitudinal fissures and transverse cracks are present. TS of bark shows rhytidoma as a major portion followed by stone cell bands and secondary phloem with groups of phloem fibres, with starch and prisms of calcium oxalate being found in all the tissues. Saponins, tannins, triterpenoids and flavanoids were found be the major components of the bark. HPTLC method was developed for quantification of betulinic acid by scanning the plates at 523nm. The quality parameters and HPTLC method developed would serve as useful tools in standardization of Albizzia lebbeck.

Keywords

Albizzia lebbeck, HPTLC, Quality Parameters, Betulinic Acid.

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