Research Journal of Pharmacognosy and Phytochemistry

John Refaat ¹, Mohamed S. Kamel ², Mahmoud A. Ramadan ³

Affiliations
1 Pharmacognosy Department, Minia University, 61519, Minia, EG
2 Pharmacognosy Department, Minia University, 61519 Minia, EG
3 Pharmacognosy Department, Assiut University, 71515 Assiut, EG

Abstract

Many Crinum species are traditionally used in different parts of the world for various local pains, inflammatory processes and microbial infections. In the present study, the total ethanolic extracts of C. augustum Rox. bulbs and C. asiaticum L. leaves were fractionated separately into five fractions each. The resulting fractions (400 mg/Kg, orally) of the total extract of C. augustum Rox. bulbs were evaluated for their analgesic and anti-inflammatory effects in mice using the hot plate and carrageenan-induced paw oedema tests versus acetyl salicylic acid (ASA) (100 mg/Kg, orally) and indomethacin (15 mg/Kg, orally), respectively. Fractions II, III and ASA showed the highest analgesic effects, whereas; II, III, IV and indomethacin were the highest antiinflammatory ones at that tested doses. On the other hand, a comparative study of the antimicrobial activities of the total extracts of both plants together with their fractions (at 5, 10 and 50 mg/ml) showed inhibitory effects on S. aureus and E. coli, especially at 50 mg/ml. In addition, the per oral LD₅₀ of the total extract of C. augustum Rox. bulbs were determined to be 1.6 g/Kg in mice.

Keywords

Acute Toxicity, Analgesic, Anti-Inflammatory, Antimicrobial, Crinum.

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John Refaat ¹, Mohamed S. Kamel ², Mahmoud A. Ramadan ³, Ahmed A. Ali ³

Affiliations
1 Pharmacognosy Department, Minia University, 61519 Minia, EG
2 Pharmacognosy Department, Minia University, 61519 Minia, EG
3 Pharmacognosy Department, Assiut University, 71515 Assiut, EG

Abstract

From the lipid fraction of Crinum asiaticum L. leaves, six saturated straight chain hydrocarbons, two sterols as well as thirteen fatty acids were isolated and identified. The isolation and identification of these compounds were based on GLC/MS technique. Most of these compounds are reported here for the first time either in this species or in Crinums generally. On the other hand, GLC/MS analysis of the volatile oil of its flowers, for the first time among Crinums, resulted in the isolation of ten components, eight of which were identified. Unsaturated fatty acids and phenols were found to prevail in the leaves and flowers' volatiles, respectively.

Keywords

Amaryllidaceae, Crinum asiaticum, Fatty Acids, GC/MS, Volatile Oils.

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