Research Journal of Pharmacognosy and Phytochemistry

B. Suneetha ¹, K. V. S. R. G. Prasad ², P. Deepthi Nishanthi ³, B. R. Soumya ³, B. Sampath Kumar ³

Affiliations
1 Department of Biotechnology, Acharya Nagarjuna University, Guntur, IN
2 Department of Pharmaceutical Sciences, Sri Padmavathi Mahila Visvavidyalayam, Tirupathi, IN
3 Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Secundrabad, IN

Abstract

The present study deals with the investigation of the hepatoprotective and antioxidant effect of methanolic extract of Actinodaphne madraspatana. Biochemical parameters like Serum Glutamate Oxaloacetate transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Alkaline phosphatase (ALP), serum triglycerides and serum bilirubin were measured. The administration of single dose of 1.25 ml/kg of carbon tetrachloride cause an increase in the serum levels of estimated biochemical parameters whereas group of rats pretreated orally with silymarin (10 mg/kg) and methanolic extracts (200 and 400 mg/kg) of Actinodaphne madraspatana for five consecutive days showed a decrease in the serum levels of biochemical parameters. Histopathological assessment were also done. Histological analysis revealed that methanolic extract of Actinodaphne madraspatana (200 and 400 mg/kg) and silymarin reduced the incidence of liver lesions, central vein necrosis, apoptosis, hepatocellular vascuolization and fatty changes caused by CCl₄. The extract significantly elevated the levels catalase, reduced glutathione and declined the levels of malondialdehyde in liver, as compared to those in CCl₄ treated group and suggests that the hepatoprotective effect of Actinodaphne madraspatana is due to the antioxidant effect of the extract. Phytochemical analysis of methanolic extract of A. madraspatana revealed the presence of alkaloids, glycosides, tannins, flavonoids and carbohydrates.

Keywords

Actinodaphne madarasapatana, Hepatoprotective, Silymarin, SGOT, SGPT, ALP, Serum Total Bilirubin, Serum Triglycerides.

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B. Suneetha ¹, K. V. S. R. G. Prasad ², B. R. Soumya ³, P. Deepthi Nishantha ³, B. Sampath Kumar ³, D. Rajaneekar ³

Affiliations
1 Department of Biotechnology, Acharya Nagarjuna University, Guntur, IN
2 Padmavathi Mahila Visvavidyalayam, Tirupathi, IN
3 Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad-500 014, Andhra Pradesh, IN

Abstract

The present study was designed to investigate the in-vitro anti-oxidant activity of methanolic (MEAM), ethyl acetate (EAEAM), chloroform (CEAM) and aqueous (AEAM) extracts of leaves of Actinodaphne madraspatana (AM). MEAM, EAEAM, CEAM and AEAM extracts of leaves were also subjected to the preliminary phytochemical screening. The in-vitro models selected for the present study are DPPH (Di- Phenyl Picryl Hydrazine) free radical scavenging method, Nitric Oxide (NO) free radical scavenging method and Reducing power assay (RPA). Ascorbic acid was used as a standard in these three methods. The first two methods include the direct measurement of free radical scavenging activity whereas the remaining method includes the evaluation of reducing power. IC50 and R2 values of MEAM, EAEAM, CEAM and AEAM were also calculated in DPPH and Nitric Oxide free radical scavenging methods. The preliminary phytochemical screening of MEAM, EAEAM, CEAM and AEAM extracts of leaveshas revealed the presence of various bioactive principles such as tannins, flavonoids, saponins and steroids. From the present study we can propose that MEAM, EAEAM, CEAM and AEAM leaf extracts possess a significant anti-oxidant activity. However, further investigations are to be undertaken in order to elucidate the mechanisms of anti-oxidant activities of leaf extracts of Actinodaphne madraspatana.

Keywords

Actinodaphne madraspatana, In-Vitro Antioxidant Activity, DPPH Method, Nitric Oxide Method, Reducing Power Assay.

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