Nano Science & Nano Technology: An Indian Journal

Open Access Open Access  Restricted Access Subscription Access

Imide Derived Carbon Dots Exhibit Promising Antitumoral Properties on Multiple In Vitro Experimental Designs


Affiliations
1 TRANSCEND Center, Regional Institute of Oncology, Iasi, Romania
2 Department of Natural and Synthetic Polymers, “Gheorghe Asachi” Technical University of Iasi, Romania
 

Cancer is still a leading cause of death worldwide. Multiple and heterogeneous intrinsic molecular defects account for malignancy aggressiveness features. Distorted and inappropriate control of fundamental cell biology programs, such as cell survival, cell suicide, cell differentiation and cell tissular architectural integration stand at the core of tumor development. Such factors activate Epithelial-to-Mesenchymal Transition (EMT) that allows tumor cells to acquire different characteristics enabling them to invade and increase survival to different type of treatments. Nanomaterial-based technologies offer new promising approaches to disease diagnostics and therapeutics. We investigate the potential antitumoral properties of one of the newest classes of carbon based nanomaterials, Carbon Dots. Two types of previously physico-chemically characterized Carbon-Dots have been assessed using in vitro models. Cell proliferation, apoptosis and vimentin as a marker of EMT have been investigated in cell cult ures models: 2D, 3D, mono-culture or co-culture. Imide derived C-Dots affect cancer cell survival in a graded way, with minimal impact on normal cells, indicating promising antitumoral effects for cancer treatment. Moreover, the presence of the imide derived C-Dots down regulates the expression of EMT-associated marker Vimentin. Tested Carbon-Dots possess selective antitumoral properties which may be defined by precursor type.

Keywords

Anti-Cancer Agent, Carbon Dots, EMT, Nanomedicine, In Vitro Evaluation.
User
Notifications
Font Size

  • Li H, Kang ZH, Liu Y, et al. Carbon nanodots: synthesis, properties and applications. J Mater Chem. 2012;22(26):24230-53.

  • Tuerhong M, XU Y, YIN XB. Review on carbon dots and their applications. Chinese J Anal Chem. 2017;45(1):139-50.

  • Molaei MJ. Carbon quantum dots and their biomedical and therapeutic applications: a review. RSC Adv. 2019;9:6460-81.

  • Stan CS, Horlescu P, Ursu LE, et al. Facile preparation of highly luminescent composites by polymer embedding of carbon dots derived from N-hydroxyphthalimide. J Mater Sci. 2017;41:185-96.

  • Stan CS, Albu C, Coroaba A, et al. One step synthesis of fluorescent Carbon Dots through pyrolysis of N-hydroxysuccinimide. J Mater Chem. 2015;3:789-95.

  • Wang M, Zhou A, An T, et al. N-Hydroxyphthalimide exhibits antitumor activity by suppressing mTOR signaling pathway in BT-20 and LoVo cells. J Exp Clin Cancer Res. 2016;35:41.

  • https://pubchem.ncbi.nlm.nih.gov/compound/N-Hydroxysuccinimide#section=GHS-Classification

  • Thiery JP, Sleeman JP. Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol. 2006;7(2):131-42.

  • Wang Y, Shang Y. Epigenetic control of epithelial-to-mesenchymal transition and cancer metastasis. Exp Cell Res. 2013;319(2):160-9.

  • Buck E, Eyzaguirre A, Barr S, et al. Loss of homotypic cell adhesion by epithelial-mesenchymal transition or mutation limits sensitivity to epidermal growth factor receptor inhibition. Mol Cancer Ther. 2007;6(2):532-41.

  • Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646-74.

  • Frisch SM, ScreatonRA. Anoikis mechanisms. Curr Opin Cell Biol. 2001;13(5):555-62.

  • Dawar DS, Kumar S, Shalini S, et al. Old, new and emerging functions of caspases. Cell Death Differ. 2015;22(4):526-39.

  • Taylor RC, Cullen SP, Martin SJ. Apoptosis: controlled demolition at the cellular level. Nat Rev Mol Cell Biol. 2008;9(3):231-41.

  • Rodrigue-Gervais IG, Saleh M. Caspases and immunity in a deadly grip. Trends Immunol. 2013;34(2):41-9.

  • Gjerdrum C, Tiron CE, Høiby T, et al. Axl is an essential epithelial-to-mesenchymal transition-induced regulator of breast cancer metastasis and patient survival. PNAS. 2010;107(3):1124-9.

  • Tadokoro A, Kanaji N, Liu D, et al. Vimentin regulates invasiveness and is a poor prognostic marker in non-small cell lung cancer. Anticancer Res. 2016;36(4):1545-52.

  • Tanaka K, Tokunaga E, Inoue Y, et al. Impact of expression of vimentin and axl in breast cancer. Clin Breast Cancer. 2016;16(6):520-6.

  • Vuoriluoto K, Haugen H, Kiviluoto S, et al. Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer. Oncogene. 2011;30(12):1436-48.

  • Satelli A, Li S. Vimentin as a potential molecular target in cancer therapy Or Vimentin, an overview and its potential as a molecular target for cancer therapy. Cell Mol Life Sci. 2011;68(18):3033-46.

  • Kokkinos M, Wafai R, Wong MK, et al. Vimentin and epithelial-mesenchymal transition in human breast cancer-observations in vitro and in vivo. Cells Tissues Organs. 2007;185(1-3):191-203.

  • Brown Y, Hua S, Tanwar PS. Extracellular matrix-mediated regulation of cancer stem cells and chemoresistance. Int J Biochem Cell Biol. 2019;109:90-104.

  • Socovich AM, Naba A. The cancer matrisome: From comprehensive characterization to biomarker discovery. Semin Cell Dev Biol. 2019;89:157-66.

  • Pérez-Velázquez J, Gevertz JL, Karolak A, et al. Microenvironmental niches and sanctuaries: a route to acquired resistance. Adv Exp Med Biol. 2016;936:149-64.

  • Wang X, Mooradian AD, Erdmann-Gilmore P, et al. Breast tumors educate stromal tissue with individualized but coordinated proteomic signatures. Sci Signal. 2017;10(491):8065.

  • Vasimalai N, Vilas-Boas V, Gallo J, et al. Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumor cell growth inhibition. Beilstein J Nanotechnol. 2018;9:530-44.

  • Savin C-L, Tiron CE, Carasevici E, et al. Entrapment of N-hydroxyphthalimide carbon dots in different topical gel formulations: new composites with anticancer activity. Pharmaceutics. 2019;11(7):303.


Abstract Views: 248

PDF Views: 0




  • Imide Derived Carbon Dots Exhibit Promising Antitumoral Properties on Multiple In Vitro Experimental Designs

Abstract Views: 248  |  PDF Views: 0

Authors

Crina Elena Tiron
TRANSCEND Center, Regional Institute of Oncology, Iasi, Romania
Florin Zugun-Eloae
TRANSCEND Center, Regional Institute of Oncology, Iasi, Romania
Catalina A. Peptu
Department of Natural and Synthetic Polymers, “Gheorghe Asachi” Technical University of Iasi, Romania
Adrian Tiron
TRANSCEND Center, Regional Institute of Oncology, Iasi, Romania
Corneliu S. Stan
Department of Natural and Synthetic Polymers, “Gheorghe Asachi” Technical University of Iasi, Romania

Abstract


Cancer is still a leading cause of death worldwide. Multiple and heterogeneous intrinsic molecular defects account for malignancy aggressiveness features. Distorted and inappropriate control of fundamental cell biology programs, such as cell survival, cell suicide, cell differentiation and cell tissular architectural integration stand at the core of tumor development. Such factors activate Epithelial-to-Mesenchymal Transition (EMT) that allows tumor cells to acquire different characteristics enabling them to invade and increase survival to different type of treatments. Nanomaterial-based technologies offer new promising approaches to disease diagnostics and therapeutics. We investigate the potential antitumoral properties of one of the newest classes of carbon based nanomaterials, Carbon Dots. Two types of previously physico-chemically characterized Carbon-Dots have been assessed using in vitro models. Cell proliferation, apoptosis and vimentin as a marker of EMT have been investigated in cell cult ures models: 2D, 3D, mono-culture or co-culture. Imide derived C-Dots affect cancer cell survival in a graded way, with minimal impact on normal cells, indicating promising antitumoral effects for cancer treatment. Moreover, the presence of the imide derived C-Dots down regulates the expression of EMT-associated marker Vimentin. Tested Carbon-Dots possess selective antitumoral properties which may be defined by precursor type.

Keywords


Anti-Cancer Agent, Carbon Dots, EMT, Nanomedicine, In Vitro Evaluation.

References