Nature Environment and Pollution Technology

Zahra Bamzadeh ¹, Majid Baserisalehi ², Nima Bahador ¹, Seyed Hossein Hejazi ³

Affiliations
1 Department of Microbiology, Fars Science Research Branch, Islamic Azad University, Fars, IR
2 Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, IR
3 Skin Diseases and Leishmaniasis Research Center, Department of Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IR

Abstract

Streptomyces is the largest genus of Actinobacteria and includes aerobic, Gram-positive filamentous bacteria with high G+C content. Although, few species of Streptomyces are pathogens, they could be characterized by production of the beneficial metabolites viz., antibiotics, antifungal, antiparasitic, and immunosuppressant compounds. The present study was conducted to isolate Streptomyces from soil and characterize their bioactive compounds. In total 85 soil samples were collected and assessed for production of the bioactive compounds by Agar Well Diffusion method against antagonistic microorganisms such as Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, Aspergillus niger and Candida albicans. Of all the Streptomyces isolates, one strain exhibited potent activity against Staphylococcus aureus, Bacillus cereus and Aspergillus niger. The promising strain identified using 16SrRNA gene sequencing and recognized as Streptomyces phaeochromogenes strain NRRL. Then, the growth phase of production and Arbitrary Unit (AU) of the bioactive compounds were determined, and the cured bioactive compound was purified and subjected to 1H NMR, 13C NMR and FTIR analysis for determination of the structural formula. The results obtained illustrated that high level of the bioactive compounds produced at stationary phase with Arbitrary Unit 32 AU. On the other hand, the data obtained from 1H NMR, 13C NMR and FTIR analysis exhibited a straight chain with a possible structural formula C₁₃H₁₅NO₃ for the bioactive compound.

Keywords

Streptomyces, Phaeochromogenes, Bioactive Compounds, Antimicrobial Activity.

Full Text

   

Nima Bahador ¹, Majid Baserisalehi ², Bita Alaei ³

Affiliations
1 Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, IR
2 Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, IR
3 Department of Mathematics, Kazeroun Branch, Islamic Azad University, Kazeroun, IR

Abstract

Pseudomonas aeruginosa is a Gram negative and motile bacterium with different virulence factors. Nowadays, the reports from worldwide illustrated antibiotic resistance of this opportunistic bacterium. Hence, the present study tried to isolate clinical and environmental samples and compare the antibiotic susceptibility as well as enzyme profiles of the isolates. For this purpose, 100 specimens from environmental sources and clinical sources have been collected. The samples were cultivated on cetrimide agar and blood agar, then the plates were incubated at 35°C for 24-48 hrs. The suspected colonies were purified and preliminarily characterized using Gram reaction, catalase, oxidase and motility test. Then they were identified using Api 20 NE kits. Afterward, antimicrobial susceptibility of the isolates was evaluated using Kirby-Bauer test, and enzyme profile were checked using API ZYM kit. The results obtained from this study indicated that out of 50 specimens, 10 isolates belonged to the environmental samples and 13 belonged to the clinical one. In addition, the highest isolation rate of clinical samples was belonging to the wound specimens. Furthermore, the results indicated that there is no difference between the enzyme profiles of the isolates, but regarding to the antibiotic susceptibility, the environmental samples were more sensitive than the clinical one.

Keywords

Antibiotic Susceptibility, Clinical Sample, Environmental Sample, Enzyme Profile, Pseudomonas aeruginosa.

Full Text