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Zhang, Kai
- Bacterial Community Composition Associated with Freshwater Cyanobacterial Blooms of Intensive Culture Ponds
Abstract Views :138 |
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Authors
Yun Xia
1,
Ermeng Yu
1,
Zhifei Li
1,
Deguang Yu
1,
Guangjun Wang
1,
Jun Xie
1,
Wangbao Gong
1,
Kai Zhang
1
Affiliations
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute of CAFS, Guangzhou, Guangdong 510380, CN
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute of CAFS, Guangzhou, Guangdong 510380, CN
Source
Nature Environment and Pollution Technology, Vol 16, No 4 (2017), Pagination: 1059-1066Abstract
To determine the composition of the bacterial communities associated with cyanobacteria in the freshwater ponds of Channa argus in intensive culture in the late culture period, we examined the bacterial community of non-cyanobacterial blooms freshwater ponds (C) and cyanobacterial blooms freshwater ponds (CB) using PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rDNA clone library. DGGE profiles showed that Microcystis sp. (JX391871.1), Leptospira sp. (JX067673.1) and Verrucomicrobium sp. (FN668203.2) existed only in CB, whereas Sphingomonas sp. (AB681542.1) and Paenibacillus sp. (JQ659563.1) were the C-specific bacteria. The 16S rDNA clone libraries of pond water showed that Actinobacteria (15 OTUs, 31.9%), Bacteroidetes (13 OTUs, 28.7%), Fibrobacteres (7 OTUs, 14.9%) and Proteobacteria (5 OTUs, 10.0%) were the dominant groups in C; the four dominant groups in CB were Bacteroidetes (17 OTUs, 34.7%), Actinobacteria (9 OTUs, 18.4%), Fibrobacteres (6 OTUs, 12.2%) and Proteobacteria (8 OTUs, 16.2%). In the clone library, distribution of sequences belonging to Fibrobacter in CB (22.2% of the 99 clones) was twice as that of C (11.1% of the 99 clones). In both DGGE map and 16S rDNA clone library the Firmicutes group (JQ659563.1 in DGGE and 3 OTUs in C library) existed only in C. These results suggested that the composition of bacterial communities changed with the cyanobacterial blooms in Channa argus intensive culture freshwater ponds, and whether the change of the Firmicutes-to-Bacteroidetes ratio could be used to predict cyanobacterial blooms in these types of ponds required further validation.Keywords
PCR-DGGE, Clone Library, Cyanobacterial Blooms, Bacterial Community, Channa argus, Culture Ponds.- Pilot-Scale Submerged Hollow Fibre Membrane Bioreactor Thickening Operation: Membrane Clogging, Sustainable Flux and Sludge Characteristics Assessment
Abstract Views :181 |
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Authors
Affiliations
1 College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, CN
2 Graduate School, Hebei University of Engineering, Handan, 056038, CN
1 College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, CN
2 Graduate School, Hebei University of Engineering, Handan, 056038, CN
Source
Nature Environment and Pollution Technology, Vol 17, No 1 (2018), Pagination: 261-268Abstract
The sludge characteristics and their impact on membrane clogging propensity and flux behaviours by statistical analyses were investigated in a pilot-scale hollow fiber membrane bioreactor during thickening operation at constant F/M ratio of 0.13. The cleaning efficiencies by chemical enhanced backflushing (CEB) and mechanical declogging at different mixed liquor suspended solids (MLSS) concentration and flux levels were further assessed through (post-cleaning) permeability decline rates. The results showed that MLSS concentration exerted the greatest impacts on the ratio of accumulated solids to mixed liquor solids loading (ΔK) and sustainable flux (JSUS), and its measurement could be used to predict both ΔK and JSUS, with a strong correlation between ΔK and JSUS observed. Moreover, membrane permeability recovery exhibited strong dependence on MLSS concentration and operational flux. On the premise of sub-critical flux operation, the recovered permeability can be sustained with a standard chemical enhanced backflushing (CEB) or declogging combined CEB at low (~8 g/L) or high (20-32 g/L) MLSS concentrations. However, the recovered permeability could only be obtained instantaneously regardless of cleaning modes when operation is exposed to super-critical flux condition, indicating the significance of the operational flux on membrane permeability sustainability.Keywords
Membrane Bioreactor, Clogging, Sustainable Flux, Sludge Characteristics, Cleaning.References
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- A New Culture Method of High Ecological Efficiency of Grass Carp (Ctenopharyngodon idellus)
Abstract Views :165 |
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Authors
Er-Meng Yu
1,
Xiao-Yan Li
2,
Jun Xie
1,
Zhang-He Chen
2,
Guang-Jun Wang
1,
De-Guang Yu
1,
Zhi-Fei Li
1,
Yun Xia
1,
Kai Zhang
1
Affiliations
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute of CAFS, Guangzhou 510380, CN
2 College of Life Science, South China Normal University, Guangzhou 510631, CN
1 Key Laboratory of Tropical & Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute of CAFS, Guangzhou 510380, CN
2 College of Life Science, South China Normal University, Guangzhou 510631, CN
Source
Nature Environment and Pollution Technology, Vol 17, No 1 (2018), Pagination: 299-305Abstract
To explore new culture methods to save aquaculture water and reduce the emission of aquaculture wastewater in current culture of grass carp mainly feeding with compound feed now, in this study, grass carp were respectively fed with hybrid napier grass (grass group), compound feed (feed group), and a mixture of hybrid napier grass and compound feed (mixture group). Growth rate, aquaculture water quality, and sediment indexes in these three groups were analysed. The results revealed that mixture group had the highest growth rate and grass group had the lowest rate (P < 0.05). The lowest levels of water TN, NH4 +-N, TP, SP, CODcr, Cyanobacteria and sediment available N, P, K were observed in the grass group, and the highest were observed in the feed group (P < 0.05). Overall, the feeding approach of compound feed mixed with hybrid napier grass might be a better model for grass carp. In the future, if hybrid napier grass can be formed into a palatable diet combined with compound feed, it will perhaps reduce the accumulation of sediments brought by the hybrid napier grass, and further enhance the utilization efficiency of the hybrid napier grass in grass carp aquaculture.Keywords
Grass Carp, Hybrid Napier Grass, Flesh Quality, Ecological Efficiency.References
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