Nature Environment and Pollution Technology

Nima Bahador ¹, Majid Baserisalehi ², Bita Alaei ³

Affiliations
1 Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, IR
2 Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, IR
3 Department of Mathematics, Kazeroun Branch, Islamic Azad University, Kazeroun, IR

Abstract

Pseudomonas aeruginosa is a Gram negative and motile bacterium with different virulence factors. Nowadays, the reports from worldwide illustrated antibiotic resistance of this opportunistic bacterium. Hence, the present study tried to isolate clinical and environmental samples and compare the antibiotic susceptibility as well as enzyme profiles of the isolates. For this purpose, 100 specimens from environmental sources and clinical sources have been collected. The samples were cultivated on cetrimide agar and blood agar, then the plates were incubated at 35°C for 24-48 hrs. The suspected colonies were purified and preliminarily characterized using Gram reaction, catalase, oxidase and motility test. Then they were identified using Api 20 NE kits. Afterward, antimicrobial susceptibility of the isolates was evaluated using Kirby-Bauer test, and enzyme profile were checked using API ZYM kit. The results obtained from this study indicated that out of 50 specimens, 10 isolates belonged to the environmental samples and 13 belonged to the clinical one. In addition, the highest isolation rate of clinical samples was belonging to the wound specimens. Furthermore, the results indicated that there is no difference between the enzyme profiles of the isolates, but regarding to the antibiotic susceptibility, the environmental samples were more sensitive than the clinical one.

Keywords

Antibiotic Susceptibility, Clinical Sample, Environmental Sample, Enzyme Profile, Pseudomonas aeruginosa.

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