Nature Environment and Pollution Technology

Xinli Lu ¹, Chun Hai Zhao ², Aijun Zhao ³, Yuqi Zhang ¹, Yichun Wu ², Suliang Chen ¹, Xiaohui Liang ⁴

Affiliations
1 Hebei Center for Disease Prevention and Control, Shijiazhuang 050021, CN
2 Binzhou Polytechnic Shan dongbinzhou 256603, CN
3 University of Business and Economics in Hebei, Shijiazhuang 050051, CN
4 Energy Research Institute, Shandong Academy of Sciences, Jinan, CN

Abstract

In this study, laccase produced by the white rot fungus strain Pycnoporus sp. Y1 was used for the leather dyeing wastewater decolorization. The mediators including veratryl alcohol (VA), 2,2'-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxybenzoic acid (HA), hydroxybenzotrizole (HOBT), and temperature, pH values, laccase concentration, and leather dyeing wastewater dilution ratio were all investigated in the experiments, and then four factors (mediators, temperatures, pH values, dilution ratios) were optimized by orthogonal experimental design. The optimal conditions for wastewater decolorization were obtained as follows: temperature of 50°C, pH 3.0, laccase concentration 4 U/mL, and the dilution ratio of 1-fold, the decolorization ratio could reach 58.42% in 10 min under the optimized conditions at last.

Keywords

Leather Dyeing Wastewater, Decolorization Laccase, Pycnoporus Sp.

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