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Maftuch,
- Effect of Crude Ethyl Acetate Extract and Fraction of Soursop (Annona muricata) Leaf on Morphology of Edwardsiella tarda with Scanning Electron Microscopy
Authors
1 Laboratory of Microbiology, University Brawijaya, ID
2 Laboratory of Fish Disease, University Brawijaya, ID
Source
Nature Environment and Pollution Technology, Vol 16, No 1 (2017), Pagination: 231-235Abstract
The objective of this research was to assess the antibacterial activity of ethyl acetate fraction extract of Annona muricata against Edwardsiella tarda, knowing antibacterial compounds and structural changes of Edwardsiella tarda by using Scanning Electron Microscopy. Method of this research used was descriptive for estimating the results of the compound in the ethyl acetate fraction of Annona muricata and morphological changes of Edwardsiella tarda, as well as experimental antibacterial activity seen from the zone of inhibition. Data obtained from the results of further studies were statistically analysed used by statistical applications. Result showed that the yellow fraction of the crude extract ethyl acetate produced the best inhibition was 9:35 mm, and MIC with a concentration of 62.5 ppm. Estimation compound ethyl acetate fraction of Annona muricata by LCMS results that corresponded to the type of compound classes anthraquinone emodin. Ethyl acetate fraction of Annona muricata showed a relatively moderate inhibitory activity against bacterial pathogens, it was Edwardsiella tarda, as well as an optimal compound act as an antibacterial can already be identified, it was from phenol derivatives.
Keywords
Antibacterial, Acetate Fraction, Annona muricata, Edwardsiella tarda, Anthraquinone.Full Text
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