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Phenanthrene-Degrading Bacteria, Acinetobacter sp. P3d from Contaminated Soil and Their Bioactivities


Affiliations
1 School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
2 Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
3 Institute Biotechnology Marine, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
 

This study was conducted to identify the indigenous active phenanthrene-degrading bacteria from phenanthrene-contaminated soil and to determine phenanthrene degradation activity of the isolated bacteria in liquid system. The minimal salt medium supplement with phenanthrene was the selective medium to isolate phenanthrene degrading bacteria. A total of nine isolates of bacteria were successfully isolated using the spread plate method. All isolates were tested for their ability to degrade phenanthrene by cultivation in a minimal salt medium supplemented with 330mg/L phenanthrene, incubated at 30°C and 150 rpm for 28 days. The biodegradation study was analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID) and the result showed that Acinetobacter sp. P3d was capable to degrade about 93.58% phenanthrene in the selective medium. The Acinetobacter sp. P3d has the capability to degrade the phenanthrene as a sole source of carbon and energy. The results revealed that this bacterial isolate was able to degrade phenanthrene significantly and it may prove to be promising bacteria for bioremediation of PAH-containing pollutant from contaminated site.

Keywords

Phenanthrene, Acinetobacter sp. P3d, Biodegradation, Bioremediation, Contaminated Soil.
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  • Phenanthrene-Degrading Bacteria, Acinetobacter sp. P3d from Contaminated Soil and Their Bioactivities

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Authors

A. Fazilah
School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
I. Darah
Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
N. Ismail
Institute Biotechnology Marine, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

Abstract


This study was conducted to identify the indigenous active phenanthrene-degrading bacteria from phenanthrene-contaminated soil and to determine phenanthrene degradation activity of the isolated bacteria in liquid system. The minimal salt medium supplement with phenanthrene was the selective medium to isolate phenanthrene degrading bacteria. A total of nine isolates of bacteria were successfully isolated using the spread plate method. All isolates were tested for their ability to degrade phenanthrene by cultivation in a minimal salt medium supplemented with 330mg/L phenanthrene, incubated at 30°C and 150 rpm for 28 days. The biodegradation study was analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID) and the result showed that Acinetobacter sp. P3d was capable to degrade about 93.58% phenanthrene in the selective medium. The Acinetobacter sp. P3d has the capability to degrade the phenanthrene as a sole source of carbon and energy. The results revealed that this bacterial isolate was able to degrade phenanthrene significantly and it may prove to be promising bacteria for bioremediation of PAH-containing pollutant from contaminated site.

Keywords


Phenanthrene, Acinetobacter sp. P3d, Biodegradation, Bioremediation, Contaminated Soil.

References