Nature Environment and Pollution Technology

Open Access Open Access  Restricted Access Subscription Access

Molecular Characterization of Native Dairy Wastewater Degrading Microbes Isolated from Dairy Industry Effluent


Affiliations
1 Department of Biotechnology, Panjab University, Sector 14, Chandigarh-160014, India
2 Department of Biotechnology, SGGS College, Sector 26, Chandigarh-160019, India
 

In this study, fifty two microbes were isolated from dairy effluent (DE) collected from effluent treatment plant of dairy industry. All the microbial isolates were screened for DE degradation efficiency by analysing physico-chemical parameters like COD, BOD, DO, TDS, salinity and conductivity. Four microbial isolates E3, E5, E11 and F5 showed more than 55% reduction in COD, BOD, DO and were selected for molecular characterization by 16S/5.8S rRNA sequencing. These efficient degraders E3, E5, E11 and F5 were identified as Serratia marcescens, Stenotrophomonas maltophilia, Brachybacetrium muris and Cunninghamella echinulata (100-99% pairwise similarity) species. The present study concluded that these strains had potential of reducing the organic load of dairy effluent and appear to be most promising organisms for bioremediation of dairy wastewater.

Keywords

Biodegradation, Molecular Characterization, 16s rRNA, Dairy Effluent.
User
Notifications
Font Size


  • APHA 1989. Standard Methods for the Examination of Water and Wastewater. American Public Health Association, Washington DC.

  • Al-Mailem, D.M., Kansour, M.K. and Radwan, S.S. 2014. Bioremediation of hydrocarbons contaminating sewage effluent using manmade biofilms: effects of some variables. Applied Biochemistry and Biotechnology, 174(5): 1736-1751.

  • APEDA 2017. Dairy Products. Available at http://apeda.gov.in/ apedawebsite/SubHead_Products/Dairy_Products.html [Accessed June 13, 2017].

  • Dhall, P., Siddiqi, T.O., Ahmad, A., Kumar, R. and Kumar, A. 2012. Restructuring BOD:COD ratio of dairy milk industrial wastewaters in BOD analysis by formulating a specific microbial seed. The Scientific World Journal, pp. 1-7.

  • Elijah, A.I., Atanda, O.O., Popoola, A.R. and Uzochukwu, S.V.A. 2014. Molecular characterization and potential of bacterial species associated with Cassava waste. Nigerian Food Journal, 32(2): 56-65.

  • Gaikwad, G.L., Wate, S.R., Ramteke, D.S. and Roychoudhury, K. 2014. Development of microbial consortia for the effective treatment of complex wastewater. Journal of Bioremediation & Biodegradation, 5(4): 1-6.

  • Haritash, A.K. and Kaushik, C.P. 2009. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. Journal of Hazardous Materials, 169(1): 1-15.

  • Jain, N., Nanjundaswamy, C., Minocha, A.K. and Verma, C.L. 2001. Isolation, screening and identification of bacterial strains for degradation of predigested distillery wastewater. Indian Journal of Experimental Biology, 39: 490-492.

  • Kim, O.S., Cho, Y.J., Lee, K., Yoon, S.H., Kim, M., Na, H., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H. and Won, S. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology, 62(3): 716-721.

  • Kumar, V., Kumar, V. and Bhalla, T.C. 2013. In vitro cyanide degradation by Serretiamarcescens RL2b. International Journal of Environmental Sciences, 3(6): 1969-79.

  • Mazzucotelli, C.A., Ponce, A.G., Kotlar, C.E. and Moreira, M.D.R. 2013. Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste. Food Science and Technology (Campinas), 33(2): 295-303.

  • Mazzucotelli, C.A., Durruty, I., Kotlar, C.E., Moreira, M.R., Ponce, A.G. and Roura, S.I. 2014. Development of a microbial consortium for dairy wastewater treatment. Biotechnology and Bioprocess Engineering, 19(2): 221-230.

  • Patel, J.B. 2001. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Molecular Diagnosis, 6(4): 313-321.

  • Porwal, H.J., Mane, A.V. and Velhal, S.G. 2015. Biodegradation of dairy effluent by using microbial isolates obtained from activated sludge. Water Resources and Industry, 9: 1-15.

  • Prasad, M.P. and Manjunath, K. 2011. Comparative study on biodegradation of lipid-rich wastewater using lipase producing bacterial species. IJB-Niscair, 10: 121-124.

  • Rainey, F.A., Ward-Rainey, N., Kroppenstedt, R.M. and Stackebrandt, E. 1996. The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. International Journal of Systematic and Evolutionary Microbiology, 46(4): 1088-1092.

  • Sawyer, C.N., McCarty, P.L. and Parkin, G.F. 2003. Chemistry for Environmental Engineering and Science. 5th ed., New York: McGraw Hill.

  • Sonune, N.A., Mungal, N.A. and Kamble, S.P. 2015. Study of physicochemical characteristics of domestic wastewater in Vishnupuri, Nanded, India. Int. J. Curr. Microbiol. App. Sci., 4(1): 533-536.

  • Sonune, N.A. and Garode, A.M. 2015. Bioremediation potential of bacterial isolates for municipal wastewater treatment. Current World Environment, 10(2): 619-625.

  • Suardana, I.W. 2014. Analysis of nucleotide sequences of the 16S rRNA gene of novel Escherichia coli strains isolated from feces of human and Bali cattle. Journal of Nucleic Acids, pp. 1-7.

  • Sumithrabhai, K., Thirumarimurugan, M., Sivakumar, V.M. and Sujatha, S. 2016. Expedient study on treatment of dairy effulent in fluidized bed reactor using immobilized microalgae. Int. J. Adv. Engg. Tech., 7(2): 231-235.

  • Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739.

  • Tewari, D., Cieply, S. and Livengood, J. 2011. Identification of bacteria recovered from animals using the 16S ribosomal RNA gene with pyrosequencing and Sanger sequencing. Journal of Veterinary Diagnostic Investigation, 23(6): 1104-1108.

  • Velmurugan, A.M. and Arunachalam, C. 2009. Bioremediation of phenol and naphthalene by Bacillus species and Brachybacterium species isolated from pharma soil sample. Current World Environment, 4(2): 299-306.

  • White, T.J., Bruns, T., Lee, S.J.W.T. and Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications, 18(1): 315-322.

  • Woo, P.C.Y., Leung, P.K.L., Leung, K.W. and Yuen, K.Y. 2000. Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient. Journal of Clinical Pathology-Molecular Pathology, 53(4): 211-215.

  • Zhang, H., Mu, W., Hou, Z., Wu, X., Zhao, W., Zhang, X., Pan, H. and Zhang, S. 2012. Biodegradation of nicosulfuron by the bacterium Serratia marcescens N80. Journal of Environmental Science and Health, Part B, 47(3):153-160.


Abstract Views: 433

PDF Views: 0




  • Molecular Characterization of Native Dairy Wastewater Degrading Microbes Isolated from Dairy Industry Effluent

Abstract Views: 433  |  PDF Views: 0

Authors

Anupama Bhardwaj
Department of Biotechnology, Panjab University, Sector 14, Chandigarh-160014, India
Jagtar Singh
Department of Biotechnology, SGGS College, Sector 26, Chandigarh-160019, India
Sonia Chaman
Department of Biotechnology, SGGS College, Sector 26, Chandigarh-160019, India

Abstract


In this study, fifty two microbes were isolated from dairy effluent (DE) collected from effluent treatment plant of dairy industry. All the microbial isolates were screened for DE degradation efficiency by analysing physico-chemical parameters like COD, BOD, DO, TDS, salinity and conductivity. Four microbial isolates E3, E5, E11 and F5 showed more than 55% reduction in COD, BOD, DO and were selected for molecular characterization by 16S/5.8S rRNA sequencing. These efficient degraders E3, E5, E11 and F5 were identified as Serratia marcescens, Stenotrophomonas maltophilia, Brachybacetrium muris and Cunninghamella echinulata (100-99% pairwise similarity) species. The present study concluded that these strains had potential of reducing the organic load of dairy effluent and appear to be most promising organisms for bioremediation of dairy wastewater.

Keywords


Biodegradation, Molecular Characterization, 16s rRNA, Dairy Effluent.

References