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Ukey, Archana
- A Study of Hematological Profile in Patients of Chronic Renal Failure Undergoing Hemodialysis at a Tertiary Health Care Institute
Abstract Views :358 |
PDF Views:94
Authors
Affiliations
1 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik-422003, Maharashtra, IN
2 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik-422003, Maharashtra, IN
1 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik-422003, Maharashtra, IN
2 Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital and Research Centre, Nashik-422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 4, No 2 (2017), Pagination: 107-112Abstract
The complete blood count, peripheral blood smear, bleeding time, clotting time and renal function tests of 114 patients of chronic renal failure who had required hemodialysis for a period of at least 3 months prior to the commencement of this study were studied. The purpose of this study was to investigate the derangements in the haematological profile of chronic renal patients undergoing hemodialysis and to correlate the same with the duration and severity of the renal failure. The principal finding in the study was a 100% prevalence of anemia among chronic renal failure patients, which was predominantly of the normocytic normochromic type. Red blood cell count was reduced in nearly all (93%) of study patients. There was also a strong negative correlation (r = -0.74) between haemoglobin and duration of hemodialysis. However, a significant proportion of cases showed abnormal cells in the peripheral blood cells such as burr cells, schistocytes and pencil cells, suggesting other contributing factors to the anemia. Female patients also showed a significantly greater prevalence of increased red cell distribution width (>15%) as compared to males (p = 0.001) and a lower prevalence of normocytic normochromic anemia as compared to males (p = 0.009). White blood cell counts, platelet counts, and bleeding and clotting times were largely within normal limits, and none of these variables showed a statistically significant association with the duration of hemodialysis, or serum creatinine, or serum blood urea nitrogen. The inference from the study is that anemia is a major comorbidity in end-stage renal failure patients, with many factors contributing to it, and detailed workup and effective treatment of anemia is necessary in this group of patients.Keywords
Anemia, Bleeding Time, Clotting Time, Complete Blood Count, Hemodialysis, Normocytic Normochromic.References
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- Rajapurkar, et al. What do we know about chronic kidney disease in India: First report of the Indian CKD registry. BMC Nephrology. 2012; 13:10. https://doi.org/10.1186/14712369-13-10 PMid:22390203 PMCid:PMC3350459
- McGonigle RJ, Shadduck RK, Fisher JW. Erythropoietin deficiency and inhibition of erythropoiesis in renal insufficiency. Kidney Int. 1984; 25:437–44. https://doi.org/10.1038/ ki.1984.36 PMid:6727139
- Subhan-ud-din, Shah SAR. Haemostatic defects in chronic kidney disease. J Med Sci. 2013 Jul; 21(3):149–52.
- World Health Organization. Iron deficiency anemia: Assessment, prevention and control. A guide for programmer manager; Geneva: WHO 2001. Available from:http://apps.who.int/iris/bitstream/10665/66914/1/WHO_ NHD_01.3.pdf?ua=1
- Palmer L, et al. ICSH recommendations for the standardization of nomenclature and grading of peripheral blood cell morphological features. Int Jnl Lab Hem. 2015; 37:287–303.https://doi.org/10.1111/ijlh.12327 PMid:25728865
- Arun S, Prabhu MV, Chowta KN, Bengre ML. The hematological pattern of the patients with chronic kidney disease in a tertiary care setup in South India. Journal of Clinical and Diagnostic Research. 2012 Aug; 6(6):1003–6.
- Chinwuba I, Uchenna I, Ngozi I. High prevalence of anemia in predialysis patients in Enugu, Nigeria. Nephrology Reviews.2010; 2:14.
- Islam MN, FerdousA, Zahid AZ, Alam M, Islam MN. Haematological profile of patients with chronic kidney disease in Northern Bangladesh. Dinajpur Med Col J. 2015 Jan; 8(1):21–7.
- Bhattacharjee K, Das D, Rabha P, Kalwar AK, Kar G, Bhattacharjee P. A study on hematological profile in patients of chronic renal failure with special reference to serum iron profile. Journal of Evidence based Medicine and Healthcare.2015; 2(46):8212–9. https://doi.org/10.18410/jebmh/ 2015/1107
- George SV, Pullockara JK, Sailesh KS, Mukkadan JK. A study to assess changes in the hematological profile inchronic kidney disease. The Pharma Innovation Journal.2015; 4(6):1–3.
- Bhatta S, Aryal G, Kafle RK. Anemia in chronic kidney disease patients in predialysis and postdialysis stages. Journal of Pathology of Nepal. 2011; 1:26–9. https://doi.org/10.3126/ jpn.v1i1.4446
- Barde R, Patel HV, Shah PR. A study of anemia prevalence in CKD patients on maintenance hemodialysis: A single centre study. Journal of Evidence Based Medicine and Healthcare. 2015; 2(39):6344–48. https://doi.org/10.18410/ jebmh/2015/871
- Hakim YAH, et al. The effect of hemodialyis on hemoglobinconcentration Platelet count and white blood cell count
- in end-stage renal failure. Int Journal of Medical Research and Health Sciences. 2016; 5(5):22–35.
- Mudiyammanavara NR, Dhananjaya PE, Agarwal R. Cross sectional study of anaemia in chronic kidney disease. Indian Journal of Basic and Applied Medical Research. 2015 Mar; 4(2):414–9.
- Reza A, Suzan S, Javad S, Mahnaz A. Hematological profile of Chronic Kidney Disease (CKD) patients in Iran, in pre-dialysis Stages and after Initiation of Hemodialysis. Saudi J Kidney Dis Transpl. 2009; 20(1):368–71.
- Tennankore KK, Soroka SD, West KA, Kiberd BA. Macrocytosis may be associated with mortality in chronic hemodialysis patients: A prospective study. BMC Nephrology. 2011; 12:19. https://doi.org/10.1186/1471-2369-12-19 PMid:21569355 PMCid:PMC3114714
- Talwar VK, Gupta HL, Shashinarayan. Clinico-haematological profile in chronic renal failure. The Journal of Association of Physicians of India. 2002; 50:228–33. PMid:12038654 20. Chandra M. Pathogenesis of the anemia of chronic renal failure: The role of erythropoietin. Nefrologia. 1990; 10.
- Cole CH. Decreased ouabain-sensitive adenine triphosphatase activity in the erythrocyte membrane of patients with chronic renal disease. Clin Sci. 1973; 45:775. https://doi.org/10.1042/cs0450775
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- Orringer EP, Mattern WDL. Formaldehyde-induced hemolysis in chronic hemodialysis. N Engl J Med. 1976;294:416. https://doi.org/10.1056/NEJM197606242942602 PMid:178999
- Rathod SG, Ade AK, Shekokar PP. A study of haematological changes in chronic renal failure. Sch J App Med Sci.2014; 2(4A):1232–4.
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- Kaw D, Malhotra D. Hematology: Issues in the dialysis patient: Platelet dysfunction and end-stage renal disease. Seminars in Dialysis. 2006; 19:317–22. https://doi.org/10.1111/ j.1525-139X.2006.00179.x PMid:16893410
- Van Bladel ER, et al. Platelets of patients with chronic kidney disease demonstrate deficient platelet reactivity in vitro. BMC Nephrology. 2012; 13:127. https://doi.org/10.1186/1471-2369-13-127 PMid:23020133 PMCid:PMC3473261
- Butt M, Shafi T, Farooq IK. Effects of dialysis on bleeding time in chronic renal failure. J Pak Med ASS. 1998; 48:242–4.
- Ramaprabha P, Bhuvaneswari T, Kumar RA. Coagulation profiles an indicator of vascular haemostatic function in chronic renal failure patients who are on renal dialysis. Sch J App Med Sci. 2014; 2(2B):592–5.
- Eberst E, Berkowitz LR. Hemostasis in renal disease: Pathophysiology and management. Am J Med. 1994; 96:168–79.
- https://doi.org/10.1016/0002-9343(94)90138-4
- Comparison of Bone Marrow Aspiration Cytology, Touch Imprint Cytology and Bone Marrow Biopsy for Bone Marrow Evaluation at a Tertiary Health Care Institute
Abstract Views :304 |
PDF Views:111
Authors
Affiliations
1 PG Resident, Department of Pathology, MVP’s Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
2 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
1 PG Resident, Department of Pathology, MVP’s Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
2 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 6, No 2 (2019), Pagination: 152-157Abstract
Introduction: Bone marrow examination is an important investigation in haematology which involves Bone Marrow Aspiration (BMA), Bone Marrow Imprint (BMI) and Bone Marrow Biopsy (BMB). Aim: Correlation of findings of bone marrow aspiration and imprint smears with biopsy in hematological disorders. Materials and Methods: It was a prospective study to correlate bone marrow examination findings by BMA, BMI and BMB of 111 patients in which bone marrow biopsy was done and correlated with aspirate and imprint smears. For aspiration Salah’s needle and for biopsy Jamshidi needle were used. Correlation of hematological and histopathological findings was done. Results: Megaloblastic anaemia was the commonest diagnosis followed by acute leukemia, nutritional anaemia, chronic leukemia, multiple myeloma, leukemia/lymphoma syndrome, and essential thrombocythemia. In case of dry aspirate or diluted marrow biopsy was useful to diagnose aplastic anemia, and secondary myelofibrosis associated with acute and chronic leukemia, multiple myeloma, leukemia/lymphoma syndrome and myelomonocytic leukemia. Aspirate and imprint smears were better for cellular morphology. Conclusion: Aspiration and imprint smears give better morphological details/cytologic diagnosis. In dry tap or diluted marrow, biopsy was more helpful in identifying architectural pattern, cellularity and fibrosis whereas imprint smears were more helpful for cellular morphology. BMA, BMI and BMB are complimentary to each other.Keywords
Anaemia, Bone Marrow Aspiration (BMA), Bone Marrow Imprint (BMI) and Bone Marrow Biopsy (BMB), Haematology, Leukemia.References
- Bain BJ, Clark DM, Wilkins BS. The normal bone marrow. In: Bain BJ, Clark DM, Wilkins BS, editors. Bone Marrow Pathology. 4th ed. Singapore: Wiley-Blackwell; 2010. p. 1-51.
- Bain BJ. Bone marrow trephine biopsy. J. Clin. Pathol. Oct 2001; 54(10):737-42. https://doi.org/10.1136/jcp.54.10.737. PMid: 11577117, PMCid: PMC1731298.
- Bearden JD, Ratkin GA, Colyman. Comparison of the diagnostic value of bone marrow biopsy and aspiration in neoplastic disease. J. Clin. Pathol. 1974; 27:738-40. https://doi.org/10.1136/jcp.27.9.738. PMid: 4426982, PMCid: PMC475459.
- Toi PCh, Varghese RG, Rai R. Comparative evaluation of simultaneous bone marrow aspiration and bone marrow biopsy: An institutional experience. Indian J. Hematol. Blood Transfus. 2010; 26:41-44.
- Sabharwal BD, Malhotra V, Aruna S, Grewal R. Comparative evaluation of bone marrow aspirate particle smears, imprints and biopsy sections. J. Postgrad Med. 1990; 36(4):194-98.
- Antonino Musolino, Annamaria Guazzi, Rita Nizzoli, Michele Panebianco, Cristina Mancini and Andrea Ardizzoni. Accuracy and relative value of bone marrow aspiration in the detection of lymphoid infiltration in non-Hodgkin lymphoma. Tumori. 2010; 96:24-27. https://doi.org/10.1177/030089161009600104. PMid: 20437853.
- Hamid GA, Hanbala N. Comparison of bone marrow aspiration and bone marrow biopsy in neoplastic diseases. Gulf J. Oncology. Jul 2009; (6):41-44.
- Mahajan V, Kaushal V, Thakur S, Kaushik R. A comparativestudy of bone marrow aspiration and bone marrow biopsy in haematological and non haematological disorders - An institutional experience. JIACM. 2013; 14(2):133-35. https://doi.org/10.7788/figurationen.2013.14.2.133.
- Tripathy S, Dudani S. Comparative evaluation of simultaneous bone marrow aspiration and trephine biopsy-Experience from routine clinical practice. Ind. J. Clin. Pract. 2013; 24(5):446-50.
- Bain BJ. Bone marrow trephine biopsy. J. Clin. Pathol. 2001 Sep; 54(9):657-63. https://doi.org/10.1136/jcp.54.9.657. PMid: 11533068, PMCid: PMC1731527.
- Kaur M, Rana A, Kapoor S, Puri A. Diagnostic value of bone [6] marrow aspiration and biopsy in routine haematological practice. J. Clin. Diagn. Res. 2014; 8(8):13-16.
- Smita Chandra, Harish Chandra. Comparison of bone marrow BMA cytology, BMI cytology and BMB for bone marrow evaluation. Hematology Reports. 2011; 3:e22.
- Aboul-Nasr R, Estey EH, Kantarjian HM, Freireich EJ, Andreeff M, Johnson BJ, Albitar M. Comparison of touch imprints with BMA smears for evaluating bone marrow specimens. American Journal of Clinical Pathology. 1999; 111(6):753-58. https://doi.org/10.1093/ajcp/111.6.753. PMid: 10361510.
- Gong X, Lu X, Wu X, et al. Role of bone marrow imprints in haematological diagnosis: A detailed study of 3781 cases. Cytopathology. Apr 2012; 23(2):86-95.
- Spectrum of Non Neoplastic Skin Diseases:A Histopathology Based Clinicopathological Correlation Study at a Tertiary Health Care Centre
Abstract Views :264 |
PDF Views:221
Authors
Affiliations
1 Professor & Head, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
2 3rd year Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
3 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
1 Professor & Head, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
2 3rd year Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
3 Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College Hospital & Research Centre, Nashik - 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 6, No 2 (2019), Pagination: 225-230Abstract
Introduction: Skin biopsy probably is the most important ancillary aid to confirm clinical diagnosis. The interpretation of many skin biopsies requires the identification and integration of two different morphological features – the tissue reaction pattern and the pattern of inflammation. Aim: To correlate histopathological diagnosis with clinical diagnosis in various non neoplastic skin lesions. Materials and Methods: The present study was a prospective and observational type of study. A total number of 197 participants were included after satisfying the eligibility criteria with due permission from Department of Dermatology. Only those patients who had given valid informed consent were included in the study. Results: Out of 197 biopsies studied, histopathological diagnosis in 167 biopsies (84.8%) was consistent with clinical diagnosis, while in 30 biopsies (15.2%) histopathological diagnosis was not consistent with clinical diagnosis. Conclusion: Out of 197 cases (M=111, F=86) biopsies studied, histopathological diagnosis was consistent with clinical diagnosis in 167 biopsies (84.8%), while in 30 biopsies (15.2%) the histopathological diagnosis was not consistent with clinical diagnosis. The skin biopsy remains the gold standard for diagnosis which can be supported with other techniques to confirm the diagnosis. This emphasizes the significance of histopathology in diagnosing non neoplastic skin disorders.Keywords
Histopathology, Clinicopathological Correlation, Non Neoplastic Skin Lesions.References
- Calonje E. Histopathology of the skin: General Principles. In: Burns T, Breathnach S, Cox N, Griffiths C, editors. Rook’s Textbook of Dermatology. 8th ed. UK: Blackwell; 2010: 10.1–10.43. https://doi.org/10.1002/9781444317633.ch10.
- Werner B. Skin biopsy and its histopathologic analysis: Why? What for? How? Part I. An Bras Dermatol. 2009; 84(4) : 391–5. https://doi.org/10.1590/S0365-05962009000400010.
- Elder DE, Murphy GF, Elinitsas R, Johnson BL, Xu X. Introduction To Dermatopathologic Diagnosis. Lever’s Histopathology of the Skin. 10th ed.
- Montagna W, Parakkal PF; The structure and function of skin. 3rd edition, New York :Springer, 1992 New Delhi: Wolters Kluwer; 2009: 1–4.
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- the skin. Indian J Dermatol 2010; 55:225–8. https://doi.org/10.4103/0019-5154.70666.
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- Mamatha. K, S. Susmitha, Vijayalaxmi. S Patil, Satyashree K. V, Disha B. S. Histopathological spectrum of dermatological lesions – An experience at tertiary care centre. IP Archieves of Cytology and Histopathology Research, 2018; (2): 83–88. https://doi.org/10.18231/2456-9267.2018.0017.
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- Yap FBB. Dermatopathology of 400 skin biopsies from Sarawak. Indian J Derm, Venr, Lepr, 2009; 75(5): 518–519. https://doi.org/10.4103/0378-6323.55407.
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- Safety of Donor and Recipient in COVID-19 Pandemic
Abstract Views :293 |
PDF Views:97
Authors
Affiliations
1 Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
2 Assistant Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
1 Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
2 Assistant Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
3 Professor and Head, Department of Pathology, Dr. Vasantrao Pawar Medical College, Hospital and Research Centre, Nashik - 422003, Maharashtra, IN
Source
MVP Journal of Medical Sciences, Vol 7, No 2 (2020), Pagination: 282-287Abstract
Novel corona virus is a new strain of corona virus family, caused by SARS-CoV-2, probably of bat origin, not previously identified in humans. The time between entry of virus in body and start of first symptoms is the incubation period of the disease. For COVID-19 the incubation period is from two days to fourteen days. COVID-19 disease spreads through small droplets from nose and mouth. Spread is also through skin contacts and even through air while speaking. Blood is an integral part of the healthcare system. During the COVID-19 pandemic blood transfusion services were affected worldwide. The blood transfusion service had to face many challenges also in India. Voluntary blood donation was decreased which is the main source of blood and blood components. In lockdown, problems of transport services, less staff, fear of getting infected all these factors affected the blood donation services. This pandemic has influenced the blood donation activities causing shortage of blood and blood components and thus affected the blood inventory. Hence the blood services had to make appropriate plans to meet the requirements of patients.Keywords
Blood Transfusion Services, Blood Inventory, Corona Virus, Pandemic, Voluntary Blood Donation, COVID-19References
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- Nagurney A. How coronavirus is upsetting the blood supply chain. Australia. The Conversation. March 12, 2020 11.05pm AEDT. Available from: https://theconversation. com/how-coronavirus-is-upsetting-the-blood-supply-chain-133424.
- World Health Organization (WHO). Maintaining a safe and adequate blood supply during the pandemic outbreak of coronavirus disease (COVID-19). Interim guidance. 2020 Mar 20.
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- Rajendran V, Pinki S. Impact of COVID-19 outbreak in blood transfusion services in India: Experience from tertiary care blood centres in Kerala: A short review. Global Journal of Transfusion Medicine. 2020 Jul 1; 5(2):215.
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- US Food and Drug Administration. Important information for blood establishments regarding the novel coronavirus outbreak.
- Available from: https://www.wsj.com/articles/how -to-donate -blood-during-the-spread-of-coronavirus-11584742912.
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