A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Panda, Amiya Kumar
- Molecular Basis of the Binding of Dye to Polycations: Absorption and Emission Spectral Studies
Authors
1 Department of Chemistry, University of North Bengal, Darjeeling-734 013, West Bengal, IN
Source
Journal of Surface Science and Technology, Vol 30, No 1-2 (2014), Pagination: 1-16Abstract
Absorption and emission spectral behaviour of an anionic xanthene dye, eosinY was investigated to perceive the nature and extent of interaction with three different cationic polyelectrolytes. The formation of dye-polymer aggregates was found to be associated with two types of interactive forces: electrostatic and hydrophobic forces. The binding constants and stoichiometry of the dye-polymer aggregates in their ground states were determined by suitably analyzing the absorption spectra by varying the concentration of the polymers. Subsequently, the thermodynamic parameters for the interaction processes were evaluated. The interaction parameters followed the sequence PDMDAAC > JR400 > LM200. The excited state interaction phenomena were studied from the fluorescence data. Change in the excited state lifetime of the fluorophore was insignificant in all the three cases. Consequently, Stern-Volmer quenching constants of the dye-polymer aggregates were calculated using the standard method. Significant information about the changes on the degree of motion of the fluorescent molecule around the polymer matrix was obtained from the anisotropy measurements.Keywords
Dye-Polymer Interaction, Fluorescence, Interaction Constant, Anisotropy.References
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- Spectral Studies On the Interaction of Toluidine Blue O With Bovine Serum Albumin
Authors
1 Department of Chemistry, Dasaratha Deb Memorial College, Khowai, Tripura, IN
2 Department of Chemistry, Tripura University, Suryamaninagar-799 130, Tripura, IN
3 Department of Chemistry, University of North Bengal, Darjeeling-734 013, West Bengal, IN
Source
Journal of Surface Science and Technology, Vol 29, No 3-4 (2013), Pagination:Abstract
Interaction between toluidine blue O (TBO) and bovine serum albumin (BSA) was investigated by UV-visible absorption and emission spectroscopy. It is proposed that the fluorescence quenching of BSA by TBO was mainly a result of the formation eof TBO-BSA complex and electrostatic interactions played an important role to stabilize the complex. The Stern-Volmer quenching constant KSV and corresponding thermodynamic parameters ΔH0, ΔG0, and ΔS0 were evaluated. Results suggest the interaction process to be comparable with the reversible biological processes. Effect of TBO on the conformation of BSA has been analyzed by means of synchronous fluorescence spectroscopy. IR spectra also proved that the interaction of TBO with BSA had changed the conformation of TBO.Keywords
Toluidine Blue O, Stern-Volmer Constant, BSA, Fluorescence Quenching.- Wiley Series on Surface and Interfacial Chemistry Ionic Liquid-Based Surfactant Science: Formulation, Characterization, and Applications
Authors
1 Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore – 721 102, West Bengal, IN
Source
Journal of Surface Science and Technology, Vol 31, No 3-4 (2015), Pagination: 198-198Abstract
No Abstract.- Physicochemical Investigation on the Pulmonary Surfactant of Some Vertebrates
Authors
1 Centre for Surface Science, Department of Chemistry, Jadavpur University, Kolkata - 700032, West Bengal, IN
2 Sansom Institute for Health Research and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, AU
3 Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore– 721102, West Bengal, IN
4 Centre for Surface Science, Department of Chemistry, Jadavpur University, Kolkata-700032, West Bengal, India, IN
Source
Journal of Surface Science and Technology, Vol 33, No 3-4 (2017), Pagination: 127-136Abstract
Large aggregate (LA) fraction of the pulmonary surfactant (PS) isolated from five different animals of the vertebrate group, lungfish, chicken, crocodile, stumpie lizard and guinea pig were isolated and characterized. Active pulmonary surfactant components were obtained by chloroform-methanol extraction of the saline suspended LA fraction. Total phospholipid (PL) and protein content were estimated biochemically by standard enzymatic methods. A systematic progression in the PL and protein content was noticed with the developmental sequence of the animals, except the crocodile, which could be due to the difficulty in the PS isolation procedure. In vitro functionality of the solvent spread film was carried out in a Langmuir surface balance by way of surface pressure (π)-area (A) measurements. PS from all the species exhibited reversible compression and expansion cycles. A clear correlation between the maximum attainable surface pressure (πmax), also known as the collapse pressure (πc) and the developmental sequence, with some exceptions, could have been established. Langmuir-Blodgett deposits, transferred onto freshly cleaved mica, were imaged by atomic force microscopy for the five different species. DPPC enriched domains showed different dimensions for the five different species. The comprehensive set of studies shed light on the composition, film functionality and structure of the pulmonary surfactants of the vertebrates where a correlation with the evolution sequence is observed.
Keywords
Cholesterol, Domain, Lipid, Protein, Pulmonary Surfactant, AFM.References
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