Journal of Pharmaceutical Research

A. B. Thomas ¹, A. A. Chaudhari ¹, R. K. Nanda ¹, L. P. Kothapalli ¹, U. B. Chavan ¹, A. D. Deshpande ¹

Affiliations
1 Department of Pharmaceutical chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune - 411 018, Maharashtra, IN

Abstract

Two spectrophotometric methods have been developed for the simultaneous estimation of Enalapril Maleate (Ena) and Losarton Potassium (Los) in combined tablet dosage forms. The first method involves determination using the absorbance correction method, the sampling wavelengths selected are, 222 nm and 250 nm over the concentration ranges of 2-32 mcg/mL and 1-60 mcg/mL for Ena and Los respectively. The second method is the second order derivative method, the sampling wavelength selected for estimation of Ena and Los are 219.5 nm and 264 nm which show linearity in the concentration ranges of 2-32 mcg/mL and 1-60 mcg/mL respectively. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guidelines. Also the developed methods were successfully employed for dissolution studies in tablet dosage form.

Keywords

Enalapril, Losartan, Absorbance Correction Method, Second Order Derivative Method, Dissolution Studies.

Full Text

   

A. B. Thomas ¹, U. B. Chavan ¹, R. K. Nanda ¹, L. P. Kothapalli ¹, A. D. Deshpande ¹

Affiliations
1 Department of Pharmaceutical chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune-411018, Maharashtra, IN

Abstract

Two UV spectrophotometric methods have been developed for the simultaneous estimation of Hydrochlorothiazide (Hctz), Atenolol (Atn) and Losartan Potassium (Los) in combined tablet dosage forms. The first method involves determination using the Area under curve, the sampling wavelength intervals selected are 274.5-270.5 nm, 226-222 nm and 252-248 nm over the concentration ranges of 0.5-30 mcg mL^-1, 1-50 mcg mL^-1 and 1-60 mcg mL^-1 for Hctz, Atn and Los respectively. The second method involves determination using the multicomponent mode of the UV visible spectrophotometer, the sampling wavelengths selected are 272.5 , 224 and 250 nm over the concentration ranges of 0.5-30 mcg mL^-1, 1-50 mcg mL^-1 and 1-60 mcg mL^-1 for Hctz, Atn and Los respectively. The results of the analysis were validated statistically and recovery studies were carried out as per ICH guidelines. The developed methods are simple, rapid, precise, accurate and can be employed for the routine estimation of Hydrochlorothiazide, Atenolol and Losartan Potassium in both bulk and tablet dosage form.

Keywords

Hydrochlorothiazide, Atenolol, Losartan Potassium, Area under Curve Method, Multicomponent Mode Method.

Full Text