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Authors
Affiliations
1 Amrita School of Pharmacy, Kochi, IN
2 Department of Oncology, Amrita Institute of Medical Sciences, Kochi, IN
3 Department of Pathology, Amrita Institute of Medical Sciences, Kochi, IN
4 Department Medical Oncology &
Hematology, Amrita Institute of Medical
Sciences, Kochi, IN
Source
Journal of Pharmaceutical Research, Vol 14, No Special Ed (2015), Pagination: 93-93
Abstract
The discovery of 1p and 19q chromosomal arms deletion in glial tumors influences both more objective diagnosis and more accurate prediction of chemotherapy response.
As a result an attempt has been made to detect deletion using fluorescence in-situ hybridization (FISH) and analyzed its prognostic value in a cohort of glial tumor patients from Amrita Institute of Medical Sciences and Research Center Kochi.
FISH was performed on 66 FFPE tissue sections by using Vyis LSI 1p36/LSI 1q25 and LSI 19p13/LSI 19q13dual coloured FISH probe sets. Signals were scored from at least 150-250 non-overlapping, intact nuclei. 163 cases were analyzed. Both 1p and 19q deletions was observed only in 28/163 (17.17%), - 1p/+ 19q deletion 80/163 (49.07%) and+1p/-19q deletion 55/163(33.74%) .
In this work presented the FISH was successfully applied to identify deletion 1p/19q. Its incidence depends on the type of diagnosed gliomas. In contrast to reported data, the present study reveals 49.07% deletion - 1p/ + 19q.
Deletions also have prognostic significance in the test group what constitutes the basis for inclusion of determining deletion 1 p/19q into diagnostic and treatment algorithm.
Keywords
FISH, Deletion 1p/19q, Glioma.
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