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The effect of sub lethal concentration 1.31 ppm and 1.84 ppm for Arsenic and Copper on the Hepatosomatic and Gonadosomatic indices of the fish Heteropneustes fossilis was studied. In mature female catfish Heteropneustes fossilis on exposure to both test metals at both higher sub lethal concentration registered marked toxicological alterations and reduction in Hepatosomatic index (HSI) and Gonadosomatic index (GSI) throughout the year in exposed fish group. However in the control group fish the values of HSI and GSI in the increasing order in relation to seasonal variation to their maximum range during pre-spawning phase. The reduced values of above parameter found directly proportional to the metal concentration and duration of the exposure. The results of the present study will be useful for selective breeding programme, conservation and sustainable fishery management of H. fossilis in its natural habitat.

Keywords

Heteropneustes fossilis, Sub-Lethal Concentration, Copper, Arsenic HSI and GSI.

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References

Akram, M., Hafeez, M.A. and Nabi, G. (1999) Histopathological changes in the kidney of a freshwater cyprinid fish. Barilius vagra following exposure to cadmium. Pak. J. Zool., 31, 77–80.

Devi, M., Gopal, V. and Ratna, G. (1991) Liverosomatic index of Channa striatus as a biomonitoring tool of heavy metal and pesticide toxicity. J. Ecotoxicol Environ. Monit., 1, 135–141

George, S.G., Pirie, B.J.S. and Coombs, T.L. (1975) Absorption, accumulation and excretion of iron protein complexes by mytilus edulis. International Conf. Heavy metals in the environment. Toronto. Ontario Canada, pp. 887.

Gobell, G. and Mills, L.J. (2002) A temperate reef fish, Tautogolabrus adspersus (Walbaum) as a potential model species for laboratory studies evaluating reproductive effects of chemical exposure. J. Environ. Toxiol. Chem., 21, 380.

Kasthuri, J. and Chandran, M.R. (1997) Sub-lethal effect of lead on feeding energetic, growth performance, biochemical composition and accumulation of the estuarine catfish, Mystus gulio (Hamilton). J. Environ. Biol., 18, 95–101.

Kaur, A. and Kaur, K. (2006) Impact of Nickel-Chrome electroplating effluent on the protein and cholesterol contents of blood plasma of Channa punctuates (Bloch) during different phases of the reproductive cycle: Abstract. J. Environ. Biol. 27.

Kumar, M., Trivedi, S.P., Mishra, A. and Sharma, S. (2007) Histopathological changes in testis of the freshwater fish Heteropneustes fossilis (Bloch) exposed to linear alkyl benzene sulphonate (LAS): Abstract. J. Environ. Biol., 28.

Moore, J.W. and Ramamoorthy, S. (1984) Heavy metals in national water and applied monitoring and impact assessment. Springar Verlag. New York, pp. 268.

Nath, A., Verma P., Chand, G.B. and Sinha, A. (2003) Degenerative changes in the ovarian cells of Clarias batrachus due to altered physico-chemical parameter of wetland J. Ecophysial. Occup. Hlth., 3, 154–160.

Patil, H.S. and Saidpur, S.K. (1989) Effect of pollution on reproductive cycles. In: reproductive cycles of India vertebrates. S.K. Saidpur (Ed.). Delhi, Allied Publishers Limited, pp. 409–426

Pragatheeswaran, V.B., Loganathan., Natarajan, R. and Venugopalan, V.K. (1992) Distribution of heavy metals and organic carbon in sediment of Madras on Visakhapatnam Mahasagar. Bull. Nat. Inst. Oceangar., 19, 39–44.

Ram, R.N. and Sathyanesan, A.G. (1984) Mercuric Chloride induced changes in the protein, lipid and cholesterol level of liver and ovary of the fish Channa punctatus. Envion. Ecol., 2, 113–117.

Ramesh, M. and Manavalaramanujam, S.K. (1992) Effects of water hardness and the toxicity of malathion on hematological parameters of freshwater fish Cyprinus carpio. J. Ecotoxicol. Environ. monit, 2, 31–34.

Saxena, A. (2000) Biochemical changes in liver and muscles of Channa gachua during reproductive cycle. Aquacult. 1, 51–56.

Saxena, R., (1986) Comparative Studies on the Gonadosomatic index in two Bottom Feeder teleosts. Indian J. Phy. Mat. Sci., 6, 39–97

Sindhe, V.R. and Kulkarni, R.S. (2004) Gonadosomatic and Hepatosomatic indices of the fresh Water Fish Notopterus notopterus (Pallas) in response to some heavy metal exponsure. J. Environ. Biol., 25, 365–368.

Singh, B.P. and Singh, V. (2007) Endosulfan female catfish, Heteropneustes fossilis (Bloch): Abstract. J. Environ. Biol., 28.

Srivastava and Saxena, A.K. (1996) Morphological Changes in Hepatocytes in Relation to Reproductive Cycle of a Fresh Water Female Catfish Heteropneustes fossilis (Bloch). J. Ad .Zool., 17, 98–104.

Wani, G.P. and Latey, A.N. (1983) Toxic effects of cadmium on the liver of a freshwater teleost Garra mullya (Skes). Cur. Sci., 52, 1034–1035.

Sub Lethal Effects of Copper and Arsenic on Some Biochemical and Hematological Parameters of the Blood in an Air Breathing Fish Heteropneustes fossilis

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Authors

Satyendra Singh ¹, A. K. Srivastava ¹

Affiliations
1 Department of Zoology, DAV College Kanpur–208001, U.P., IN

Source

Journal of Ecophysiology and Occupational Health, Vol 15, No 3-4 (2015), Pagination: 105-114

Abstract

Study was undertaken to evaluate biochemical and hematological alterations resulting from the exposure of Heteropneustes fossilis to both sub-lethal concentration of copper (1.84 ppm and 1.10ppm) and arsenic (1.10 ppm and 0.78ppm) on various tissues for 15 days, 30 days and 60 days. Three groups of 14 fish were taken as control group, arsenic exposed group and copper exposed group. At the end of experiment periodically blood sample were taken from the control and experimental group of fish. Blood was assayed for selected biochemical and hematological parameters (hematocrit, haemoglobin per cent red blood cell counts, white blood cell counts, MCV, MCH, MCHC, clotting time, plasma protein, plasma glucose, blood plasma GOT, GPT, alkaline phosphatase, cholesterol, triglycerides). The hematological indices of both sub-lethal concentration of both test metal indicated a dose-dependent decrease in hemoglobin values, hematocrit values, red blood counts, MCH, MCHC, which are indication of anemia of the normal chronic type. Clotting time significantly increased in copper exposed fish. A dose-duration dependent increase was observed in MCV and WBC count. Biochemical analysis demonstrated the blood plasma level of protein and glucose was lowered in the exposed fish as compared to the control group, but a significant dose dependent elevation was recorded in all other bio chemical parameters. During exposure time a relative decrease in blood glucose level and an increase in blood serum cholesterol level and SGOT, SGPT, alkaline phosphatase, triglycerides level was increased. The result of present study demonstrates the toxic effect of both test metal in test fish Heteropneustes fossilis and recorded hematological and biochemical parameters can be used as an indicator of copper and arsenic related toxicological stress in fish on been exposed to sublethal concentration of both metal.

Keywords

Copper, Arsenic, Biochemistry, Hematology, Hetropneustes fossilis, Toxicity.

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References

Agrawal, S.J., Srivastava, A.K. and Chaudhary, H.S. (1979) Hematological effects of nickel toxicity on a fresh water teleost. Colisa fasciatus. Acta. Pharmacol. Toxicol., 45, 215–217

APHA, AWWA and WPCS. (1998) Standard method for the examination of water and waste water, (20th ed.), Washington D.C.

Bell, G.H., Davidson, J. and Smith, D.E. (eds.) (1972) The formed elements of the blood. In Textbook of Physiology and Biochemistry. Churchill Livingstone, Edinburgh, pp. 415–443.

Collivin, L. (1984) The effect of copper on maximum respiration rate and growth rate of perch. Perrca fluviatilis L. Water Res. 18, 139–144.

Dacie, J.A. and Lewis, S.M. (1977) Practical hematology. J and Churchill Ltd., London.

Desai, H.M., Nanda, B. and Panigrahi J. (2002) Toxicological effects on some biochemical parameters of fresh water fish Channa punctatus under the stress of Nickle. J. Environ. Biol., 23, 275–277.

Garg, V.K., Garg, S.K. and Tyagi, S.K. (1989) Manganese induced haematological and biochemical anomalies in Heteropnestes fossilis. J. Environ. Biol., 10, 349–353.

Gautam, R.K. and Thakur, P. (2003) Heavy metals impact on enzyme activites in Labeo rohita : Uttar Pradesh. J. Zool., 23, 145.

Kasthuri, J. and Chandran, M.R. (1997) Sub-lethal effect of lead on feeding energetic, growth performance, biochemical composition and accumulation of the estuarine catfish, Mystus gulio (Hamilton). J. Environ. Biol., 18, 95–101.

Kothari, S. and Soni, R. (2002) Effects of polyunsaturated fatty acids on zinc induced disturbances in the Heteropneustes fossilis intestine. Indian J. Environ. Toxicol., 12, 40–42.

Kothari, S., Bhalerao, S. and Jat, D. (2003) Protection by herbal compounds against toxic effects of mercuric chloride in fish intestine. J. Ecophysiol. Occup. Hlth., 3, 51–58.

Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and. Randoll, R.J. (1951) Protein measurements with the folin phenol reagent. J. Biol. Chem., 193, 265.

Pradhan, A.K. and Hota, A.K. (1993) Mercuric chloride toxicity and biochemical parameter of liver of Channa punctatus (Bloch.). J. Appl. Zool. Res., 4, 167–170.

Qladimeji, A.A. (1984) Long term effects of arsenic accumulation in Rainbow trout (Salmo gairdeneri). Bull. Environ. Contam. Toxicol., 32, 732.

Ram, R. and Sathyanesan, A.G. (1984) Mercuric Chloride induced changes in the protein, lipid and cholesterol of the liver and ovary of the fish Channa punctatus. Environ. Ecol., 2, 113–117.

Reddy, S.J., Kalakarni, V., Tharakanadha, B., Reddy, D.C. and Ramamurthi, R. (1998) Changes in energy metabolism of the fish Labeo rohita in relation to prolonged lead exposure and recovery. J. Ecotoxicol. Environ. Monit., 8, 45–53.

Shandilya, S. and Banerjee, V. (1989) Effect of sub-lethal toxicity of Zinc and chromium on peripheral haemogram in the fish, Heteropneustes fossilis. Environ. Ecol., 7, 16–23.

Shastry, K.V. and Gupta, A. (1994) Haematological study on the effects of cadmium and dimethoate alone and combination a fresh water teleost Channa punctatus. J. Environ. Poll., 1, 133–139.

Singh, M. (1995) Hematological responses in a freshwater teleost Channa punctatus to experimental copper and chromium poisoning. J. Environ. Biol., 16, 339–341.

Singh, R.K., Trivedi, S.P. and Sethi, N. (1992) Sumithion induced changes in the blood of catfish, Clarias batrachus. Biol. Memoris., 18, 57–59.

Sivakami, R., Premkishore, G. and Chandran, M.R. (1994a) Effect of chromium on metabolism and biochemical composition of selected tissues in the freshwater catfish Mystus vittatus. Environ. Ecol., 12, 259–266.

Srivastava, A.K., and Agrawal, S.J. (1979) Hematological anomalies in a fresh water teleost, Colisa fasciatus, on acute exposure to cabalt. Acta. Pharmacol. Toxicol., 44, 197-199.

Srivastava, R.K., Singh, H.S. and Srivastava, S.J. (2001) Physiological changes in freshwater catfish, Heteropneustes fossilis following exposure to cadmium. J Ecophy. and Occup-Health.

Srivastava, R.K. and Srivastava, S. (2002) Cadmium (Cd) induced Haematological changes in a freshwater catfish Heteropneustes fossilis. J. Adv. Zool., 23, 23–29.

Srivastava, S.J., Singh, N.D., Srivastava, A.K. and Sinha, R. (1995) Acute toxicity of malachite green and its effects on certain blood parameters of a catfish, Heteropneustes fossilis. Aquatic. Toxicol, 31, 241–247.

Srivastava, S.J., Singh, N.D., Sinha, R. and Srivastava, A.K. (1996) Acute toxicity of Fuschin basic (magenta) and its affects on blood parameters of a freshwater catfish, Heteropneustes fossilis, J. Adv. Zool., 17, 38–43.

Subhadra, G. and Sastry, K.V. (1985) Alteration in the rate of intestinal absorption of some nutrients due to zinc in the freshwater catfish H. fossilis. J. Environ. Biol. 6, 139–146.

Syverson, T.L.M. (1981) Effects of methyl mercury on protein synthesis in vitro. Acta. Pharmacol. et. Toxicol., 49, 422–426.

Tandon, R.S. and Chandra. (1978) Effects of asphyxiation stress on serim transaminases (GOT and GPT) level of freshwater catfish Clarias batrachus. Z. Tierphysial. Tierernat. U. Futtermitelkde. pp. 34–53.

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