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Verma, Prakriti
- Fluctuation of Serum TSH, FSH, Vitellogenin Level and Aassociated Ovarian Toxicity in Clarias batrachus (Linn.) Exposed to Pesticide
Abstract Views :721 |
PDF Views:313
Authors
Affiliations
1 Department of Zoology, Patna University, Patna, Bihar, IN
2 Department of Biochemistry Patna University, Patna, Bihar, IN
1 Department of Zoology, Patna University, Patna, Bihar, IN
2 Department of Biochemistry Patna University, Patna, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 14, No 3-4 (2014), Pagination: 109-120Abstract
A comprehensive study of serum Thyroid Stimulating Hormone (TSH), Follicle Stimulating Hormone (FSH) and Vitllogenin level of control and Rogor (1μl/L and 2.5μl/L) treated Clarias batrachus were done by ELISA. The present study revealed a wave of hormone imbalance and significant variations in the concentration of serum TSH, FSH and Vitellogenin level due to Rogor toxicity. The mean value of Thyroid Stimulating Hormone (TSH) level varied between 0.2 ± 0.089μIU to2.8 ± 0.141μIU after Rogor exposure as compared to normal 2.255 ± 0.187μIU value. Whereas, the mean value of Follicle Stimulating Hormone (FSH) fluctuate between 54.167 ± 3.017μIU to 181.883 ± 0.479μIU in comparison to normal value 144.5 ± 0.187 μIU. The mean value of vitellogenin ranges between 0.001 ± 0.001IU to 2.86144.5 ± 2.94IU in comparison to normal (2.24 ± 3.31 IU) value. Light and Electron microscopy studies of ovarian tissue of Rogor treated fish have shown various treatment related abnormalities. Under Light microscopy ovarian tissue of normal C. batrachus showed different stages of development in goocytes in the ovigerous lamellae extended from well developed germinal epithelium along with a few interstitial nutritive cells with distinct nucleus and nucleolus.However, Rogor exposure for 5 days, showed ruptured ovigerous lamellae, fusion of a number of young and maturing oocyte, shrinkage of nucleus and peripheral vacuolization which increased after 10 days and 15 Days exposure in both doses of Rogor (1μl/L and 2.5μl/L).Under Scanning electron microphotograph, three dimensional structures of ovarian cells also revealed grooves, pappilliations and protuberances on the surface of the ova which increased with dose and duration of exposure.Keywords
Clarias Batrachus, Dimethoate, FSH, TSH, Vitellogenin, Ovary.Full Text
- Alteration in Oxidative Stress Biomarkers and Cytoarchitecture of Hepatic Tissues in Freshwater Fish Clarias batrachus (Linn.) under Sub lethal Butachlor Stress: Spectrophotometric and TEM Study
Abstract Views :332 |
PDF Views:229
Authors
Affiliations
1 Department of Zoology, Patna University, Patna – 800005, Bihar, IN
1 Department of Zoology, Patna University, Patna – 800005, Bihar, IN
Source
Journal of Ecophysiology and Occupational Health, Vol 21, No 2 (2021), Pagination: 56-63Abstract
The present study addresses the deleterious impact of sub lethal exposure of butachlor 2-chloro-N-(2-6-diphenyl) acetamide on hepatic cells of air breathing fish Clarias batrachus (Linn.) based on light and transmission electron microscopy and estimation of oxidative stress biomarker enzymes viz. reduced glutathione and lipidperoxidase. Fishes were exposed to 1.0µl/L butachlor for 5, 10 and 15 days respectively. After schedule exposure, blood sample were collected and extracted serum were analyzed for quantitative estimation of serum reduced glutathione and lipid peroxidase activity by spectrophotometer. The liver tissues were processed for light and electron microscope. Light photomicrographs of hepatic cells reveal dose related abnormalities which increases with the duration of exposure. Major changes attributed to the hepatic cell were parenchyma degeneration, necrosis along with fibrosis, widening of sinusoids, vacuolation, and infiltration of eosinophilic inclusion, karyolysis, pyknosis and perivenular congestion. Transmission electron microscopy of hepatic cells also revealed degenerated hepatic parenchyma, accumulation of lipid and electron dense material, degenerated mitochondria, nuclear shrinkage and enlarged lysosomes engulfing cytoplasmic particles in contrast to control fish. On prolonged exposure, the most frequent pathological modifications were mitochondrial swelling with regression of cristae and giant lysosome with myelinated phospholipid membrane pointing towards phospholipidosis. The activities of all the marker enzymes showed high fluctuation indicating significant imbalance in comparison to control. The study highlights the oxidative stress caused by butachlor correlated with histopathological anomalies of hepatic cells. It can be used as sensitive index for assessing the magnitude of oxidative damage and physiological dysfunction of experimental fish under laboratory condition.
Keywords
Butachlor, Clarias batrachus, Histopathology, Liver Cell, Lipid Peroxidase, Reduced Glutathione.Full Text
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