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A comprehensive study of serum Thyroid Stimulating Hormone (TSH), Follicle Stimulating Hormone (FSH) and Vitllogenin level of control and Rogor (1μl/L and 2.5μl/L) treated Clarias batrachus were done by ELISA. The present study revealed a wave of hormone imbalance and significant variations in the concentration of serum TSH, FSH and Vitellogenin level due to Rogor toxicity. The mean value of Thyroid Stimulating Hormone (TSH) level varied between 0.2 ± 0.089μIU to2.8 ± 0.141μIU after Rogor exposure as compared to normal 2.255 ± 0.187μIU value. Whereas, the mean value of Follicle Stimulating Hormone (FSH) fluctuate between 54.167 ± 3.017μIU to 181.883 ± 0.479μIU in comparison to normal value 144.5 ± 0.187 μIU. The mean value of vitellogenin ranges between 0.001 ± 0.001IU to 2.86144.5 ± 2.94IU in comparison to normal (2.24 ± 3.31 IU) value. Light and Electron microscopy studies of ovarian tissue of Rogor treated fish have shown various treatment related abnormalities. Under Light microscopy ovarian tissue of normal C. batrachus showed different stages of development in goocytes in the ovigerous lamellae extended from well developed germinal epithelium along with a few interstitial nutritive cells with distinct nucleus and nucleolus.However, Rogor exposure for 5 days, showed ruptured ovigerous lamellae, fusion of a number of young and maturing oocyte, shrinkage of nucleus and peripheral vacuolization which increased after 10 days and 15 Days exposure in both doses of Rogor (1μl/L and 2.5μl/L).Under Scanning electron microphotograph, three dimensional structures of ovarian cells also revealed grooves, pappilliations and protuberances on the surface of the ova which increased with dose and duration of exposure.

Keywords

Clarias Batrachus, Dimethoate, FSH, TSH, Vitellogenin, Ovary.

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