Journal of Ecophysiology and Occupational Health

Risha Jasmine Nathan ¹, P. Sharma ¹, Lily Saroj Nathan ²

Affiliations
1 Forensic Chemistry and Toxicology, Lok Nayak Jayaprakash Narayan National Institute of Criminology, New Delhi, IN
2 Ewing Christian College, University of Allahabad, IN

Abstract

Muscle relaxants are occasionally encountered in forensic toxicological cases for identification and quantitation. Due to unavailability of human samples, the analysis of the muscle relaxant vecuronium was done using post-mortem rat visceral samples. Rats were injected with bolus overdose of the drug and the post mortem samples were subject to ion-pair extraction with metanil yellow dye. Method for identification of the drug by LC-MS-MS was established first with secondary standard aqueous vecuronium solutions and then applied on the questioned samples. Quantitation was done to find the quantity of the drug in liver and kidney samples.

Keywords

Aminosteroid, Muscle Relaxant Vecuronium, Liquid Chromatography-Tandem Mass Spectrometry.

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