Journal of Environment and Sociobiology

Sankar Kumar Ghosh ¹, Prabal Ranjan Ghosh ², Tarikul Huda Mazumder ¹, Miranda Kshetrimayum ¹

Affiliations
1 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
2 Department of Vetarinary Physiology, WBUAFS, Kolkata-700 037, W.B., IN

Abstract

Northeast India is a reservoir of rich biodiversity, especially Greater one-horned rhinoceros (Rhinoceros unicornis) which is facing extinction. DNA barcoding being an exciting tool for accurate documention of biodiversity with a gene sequence, in the present article DNA barcode of R. unicornis is determined for the first time by amplifying mitochondrial COI gene region using cross species primer designing and other bioinformatics analysis (Accession No. JN41700). Phylogeny analysis of obtained sequence with other related species distinctly maintains the assigned taxonomic position.

Keywords

DNA Barcoding, Mitochondrial DNA, COI-5', Rhinoceros Unicornis

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Prabal Ranjan Ghosh ¹, Pradosh Mahadani ², Rosy Mondal ², Pradip Kumar Das ¹, Sankar Kumar Ghosh ²

Affiliations
1 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
2 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN

Abstract

Poultry biodiversity widely prevails over the world, especially in Indian subcontinent as domestic indigenous fowl. Cross species hybridization of different Gallus species confers ambiguity in proper classification. Molecular approach using DNA barcoding to identify species boundaries is successfully employed to detect hybridization signal of nondescript indigenous fowls. In the present study DNA barcoding region of cytochrome oxidase I (COI) gene collected from geographically distant region of five agroclimatic zones of eastern India is amplified using PCR, sequenced and subsequently deposited in GenBank. Related sequences were retrieved from global databases for phylogenic analysis using bioinformatics. The present study provides for the first time the DNA barcodes of domestic fowls from eastern India and established that the domestic fowls are grouped within the group Gallus gallus. Additional COI sequences within the same and related genus collected from different countries properly discriminate and assign their correct taxonomic positions. Low level of interspecies variation was observed between Gallus gallus and Gallus sonneratii indicating the failure to discriminate them as separate species.

Keywords

DNA Barcoding, Poultry, Gallus Gallus, MtDNA, Cytochrome Oxidase I, Genbank.

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Prabal Ranjan Ghosh ¹, Monika Ahanthem ², Pradosh Mahadani ², Amalesh Choudhury ³, Sankar Kumar Ghosh ²

Affiliations
1 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
2 Department of Biotechnology, Assam University, Silchar-788011, IN
3 S. D. Marine Institute, Sunderban, West Bengal, IN

Abstract

Chicken domestication plays an important role for major supply of animal protein throughout the world. In India, wide diversities of domestic chicken are descended from wild red jungle fowl (RJF) (Gallus gallus), the mother of all fowls of the world. In the present study, DNA barcode sequence and cytochrome c oxidase subunit I (COI) region of wild RJF from northeastern India using PCR are determined, sequenced and subsequently deposited in GenBank (Accession No. JN793568). The study provides for the first time the DNA barcodes of red jungle fowl from northeastern India and subsequent bioinformatics yield that the RJF is grouped within correct taxonomic assignment of other Gallus species proving strong resolving power of DNA barcode in chicken.

Keywords

Cytochrome C Oxidase Subunit I, DNA Barcodes, Red Jungle Fowl, Gallus gallus, Northeastern India.

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