Journal of Environment and Sociobiology

Sagnik Kumar Ganguly ¹, Arindam Chakraborty ², Gopinath Baur ³, Sanat Roy ³, Subrata Trivedi ³, Kunal Sarkar ³

Affiliations
1 1 Debitala lane, Ichapur, 24-Parganas (N), West Bengal 743144, IN
2 Department of Serculture, Krishnath College, Berhampore, West Bengal, IN
3 Post Graduate Department of Serculture, Krishnath College, Berhampore, West Bengal, IN

Abstract

Total Hemocyte Count in spinning silkworm, Bombyx mori L. was studied using four popular multivoltine races of India. Hemocytic concentration of larva was gradually decreased in every spinning day then again increased with pupal maturity. It was a common character for all the four races in the larva to pupa transition stage. Spherulocyte abundance and prohemocyte absence are other general features in the pre-pupal stage in these four races. It is shown that spherulocytes are derived from granulocytes.

Keywords

Hemocyte, Pre-Pupal Stage, Bombyx mori.

Full Text

     

Sagnik Kumar Ganguly ¹, Arindam Chakraborty ², Gopinath Baur ², Susanta Kumar Chattopadhyay ², Subrata Trivedi ², Sudeshna Ghoshal ²

Affiliations
1 1 Debitala Lane, Ichapur, 24-Parganas (N), W. Bengal PIN- 74314, IN
2 Department of Sericulture, Krishnath College, Berhampore, West Bengal, IN

Abstract

Comparative cellular immune responses between commercially exploited bivoltine and multivoltine races have been studied. Worms of different races are inoculated with pathogenic bacteria Staphylococcus vitulinus. Total Haemocyte Count (THC) at each post-inoculation period has been noted and compared with control groups. Bivoltines have more hemocytic concentration than that of any multivoltine races. A common behavior is observed for all races after inoculation of pathogen. The hemocyte population decreases in Ist hour, increases in 2^nd hour and again decreases in 3^rd hour. In second experiment, a multivoltine race, M₁₂W was selected and was injected with saline having silica, indigo and carbon particles and with particle free saline (0.67% NaCl). But results similar to those of the first experiment were found indicating that pathogenicity is not the cause of first 3 hours, down-up-down sequence of THC in inoculated worms.

Keywords

Total Haemocyte Count, Pathogen, Bivoltine and Multivoltine Silkworms.

Full Text