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Anusha, Narlagiri
- Transforming Growth Factor β: Cloning and Expression Profiling in the Brain of Catfish Model
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Authors
Affiliations
1 Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad - 500046, Telangana, IN
1 Department of Animal Biology, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad - 500046, Telangana, IN
Source
Journal of Endocrinology and Reproduction, Vol 26, No 1 (2022), Pagination: 25-33Abstract
Transforming growth factor-beta (tgf-β) is implicated as a signalling molecule and considered a vital regulator for neuronal survival, development, ovarian growth, and many other biological processes. The present study was aimed to analyze the expression of tgf-β and its significance during the early brain and gonadal development. Initially, cloning of partial cDNA of tgf-β from the female brain was performed followed by expression analysis during various developmental stages using quantitative real-time PCR. Cloned tgf-β is approximately 210 bp, which encodes a putative protein of 69 amino acid residues, and showed high similarity with other counterparts. The tissue-specific expression of tgf-β was ubiquitous but significantly higher in brain as well as gonads of both male and female. Further, in ontogeny analysis, tgf-β mRNA levels were significantly greater in 75-, 100- and 125-days post-hatch during development. The pre-spawning stage showed higher expression of tgf-β when compared to all other reproductive stages. Immunolocalization study revealed the presence of Tgf-β protein in the preoptic area-hypothalamus which was well correlated with the mRNA expression in discrete regions of the catfish brain. Expression of tgf-β seems to have a role in the regulation of gonadal function through the brain-pituitary axis.Keywords
Brain, Development, Expression, Signalling Molecule, Tgf-β
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