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Authors
Affiliations
1 Department of Ecology and Environmental Sciences, Pondicherry University, Pudducherry, U.T., IN
Source
International Journal of Plant Sciences, Vol 8, No 1 (2013), Pagination: 144-147
Abstract
The tubers of water lily( Nelumbo nucifera )were collected from a tropical lake (Ossudu lake) and the methanol extract is screened for lectin-like substances using agglutination assay. The highest agglutination activity was found with human blood group B+ve with a maximum titre of 211 among human group O and B+ve, goat and chick erythrocytes. To ascertain the molecular mass of the protein present in the extract, one-dimensional SDS-PAGE was performed using standard methods on the Bio-Rad Mini-Protean II system. The electrophoretic gel revealed that the crude extract of tuber of Nelumbo nucifera was found to possess a protein with a molecular mass of 19 kDa.
Keywords
Nelumbo Nucifera, Hemagglutination, SDS-page, Lectin
