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Muthamilan, M.
- Prevalence, Symptomatology, Pathogenicity and Nutritional Requirements of Fusarium oxysporum f.sp.phaseoli Causing Fusarium Yellows of French Bean in Thandikudi
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Authors
Affiliations
1 Department of Plant Pathology, Agricultural College and Research Institute, Madurai (T.N.), IN
2 Department of Horticulture, Agricultural College and Research Institute, Madurai (T.N.), IN
1 Department of Plant Pathology, Agricultural College and Research Institute, Madurai (T.N.), IN
2 Department of Horticulture, Agricultural College and Research Institute, Madurai (T.N.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 161-166Abstract
Fusarium yellows/wilt caused by Fusarium oxysporum f.sp.phaseoli are one of the serious diseases in French bean in Thandikudi and Kodaikanal hills of Dindugal district was found to prevalent in all French beans growing areas under survey with maximum of 71.25 per cent incidence and 65.88 per cent disease severity at Thandikudi village of Dindugal district in Tamil Nadu. The pathogenicity test was conducted by artificial inoculation of test fungus into the young two leaf stage of French bean and the typical symptom were produced after twelve days after inoculation. Among the different solid and liquid media tested oat meal agar potato dextrose agar medium supported growth of the casual fungus. Fungus could grow well at a pH 4.0 and 5.0, respectively. This pathogenic fungus grew maximum when basal medium was supplemented with lactose and dextrose as carbon sources showed maximum growth and with potassium nitrate and sodium nitrate as nitrogen sources.Keywords
Fusarium oxysporum f.sp.phaseoli, Pathogenecity, Thandikudi, Symptoms.References
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- Screening of M3 Mutants for Yellow Vein Mosaic Virus Resistance in Greengram [Vigna radiata (L.) Wilczek]
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Authors
Affiliations
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
2 Department of Plant Pathology, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
1 Department of Plant Breeding and Genetics, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
2 Department of Plant Pathology, Agricultural College and Research Institute (T.N.A.U.), Madurai (T.N.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 265-269Abstract
Greengram [Vigna radiata (L.) Wilczek] is a cheap source of dietary protein for the poor, with high levels of folate and iron compared with many other legumes. Mungbean yellow mosaic virus is also one of the destructive viral disease affecting yield potential of greengram both quantitatively and qualitatively. Induced mutations, have offered a single and short alternative to conventional breeding including isolation, screening, selection and testing generation after generation. An investigation was carried out in two mungbean genotypes viz., CO (Gg) 7 and NM 65 treated by two mutagens viz., gamma rays at the doses of 300, 400 and 500 Gy and EMS treatments of 10, 20 and 30 mM. The trial was conducted in the research farm of Agricultural College and Research Institute, TNAU, Madurai during Kharif season 2013. The M2 generation was raised as individual M1 plant basis. The treated and control populations of M2 generation were carefully screened for pod shattering resistance. The yellow vein mosaic virus disease (YMV) incidence was recorded for all the plants in M3 generation for the selected 22 mutants. Based on field scoring, the mutants viz., M5, M18, M26, M46, M54, M58, M70, M71, M92 and M98 were identified as yellow vein mosaic virus resistant mutants. The mutants which showed field resistance were checked for YMV resistant gene using the gene specific primer ISSR811. Five mutants confirmed with marker studies. These resistant mutants have been forwarded to next generation for further yield performance.Keywords
Mungbean, Induced Mutation, Yellow Vein Mosaic Virus, Scoring, Mutants, Marker.References
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