International Journal of Plant Protection

M. Sivaji ¹, R. Vinoth ², P. Tamilkumar ³, S. Suresh Kumar ¹, A. Chandrasekar ¹, M. Syamala ⁴

Affiliations
1 Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
2 Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
3 Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN
4 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

In this study, a total of eight Burkholderia isolates obtained from rhizosphere soil of maize, cotton, rice, sugarcane, groundnut and soybean and tested for the N fixation, phosphate solubilisation and antagonistic activity against Rhizoctonia solani. All the eight isolates produced the band for the nifH gene in the polymerase chain reaction confirmed the presence of nifH gene. Among the 8 isolates, RMS1 produced clear halo zone around 7 mm on surface of HAP medium. CMS1, RMS1 and SMS1 are inhibited the growth of Rhizoctonia solani for 43, 40 and 35 per cent, respectively. An isolate RMS1 possessed all the three plant growth promoting traits viz., higher phosphate solubilizing ability, antagonistic activity and presence of nifH gene selected for molecular characterization with amplifying and sequencing of 1.46 kb 16S rRNA gene. Based on the BLASTn homology it was found that 16S rRNA sequence of isolate RMS1 having 93 per cent identity with showed 97 per cent homology with Burkholderia thailandensis.

Keywords

Plant Growth Promoting Rhizobacteria, Burkholderia sp., Polymerase Chain Reaction.

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R. Vinoth ¹, P. Jayamani ¹

Affiliations
1 Department of Pulses, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore (T.N.), IN

Abstract

Blackgram (Vigna mungo) is fourth important pulse crop of India. Major constraints in achieving higher yield of blackgram are absence of suitable ideotypes for different cropping systems, poor harvest index and susceptibility to pests and diseases. Genetic maps can be constructed based on mapping population. Among the mapping populations, Recombinant Inbred Lines (RIL) is more advantages than others. In the present study, an inter sub specific RIL population was developed by crossing VBN (Bg) 4 x Vigna mungo var. silvestris 22/2 by single seed decent method. A total of 195 RILs were evaluated for 11 biometrical traits. RIL 113 recorded the highest mean for single plant yield (9.90 g) along with more number of pods plant (42.84). RIL 125 recorded the maximum yield of 8.93g per plant along with more number of clusters per plant (17.50) and RIL 131 had single plant yield of 7.99 g along with more number of pods per plant (34.00). RILs with high yield and yield attributing traits can be used to develop high yielding varieties. RILs isolated with specific traits could be used as pre breeding material for the improvement of blackgram.

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