International Journal of Plant Protection

Anchal Rani ¹, Pragati Misra ¹, Jitendra Singh ², Pankaj Kumar ², Rosy Rani ¹, Pradeep Shukla ¹

Affiliations
1 Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (U.P.), IN

Abstract

During survey of papaya fields in Meerut, severe symptoms of leaf yellowing, intervenial chlorosis, and curl apical necrosis were observed in approximately 25 per cent of plants in each of the papaya fields. DNA extracted from leaf, midrib and bark of symptomatic and healthy plants of papaya were preceded with universal primer pairs. Expected ~1600 bp fragments were amplified with primer pair P1/Tint in diseased plants. Infection of phytoplasma disease in papaya plants was confirmed by the PCR.

Keywords

Papaya, Phytoplasma, Curl Apical Necrosis, Pcr

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Namrata Kumari ¹, Pragati Misra ¹

Affiliations
1 Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN

Abstract

Production of Banana is constrained by many biotic as well as abiotic factors. The production of disease free planting material of banana by meristem culture technique is an urgent need.An experiment has been designed to develop a suitable and efficient protocol for plant regeneration in banana through micro propagation.The surface sterilization of banana plant is the most difficult practice.So it is very important to optimize the concentration and duration of different sterilants for explants surface sterilization. The best sterilants for the surface sterilization of the explant of banana were 0.1 per cent HgCl₂ for 5 min duration followed by NaOCl (40 %, commercially available) solution for 8 min duration and 70 per cent ethyl alcohol for 2 min. And the best medium for establishment of banana explant was supplemented with 3mg/l BAP, 0.2mg/l IAA with 30 mg ascorbic acid followed by Proliferation with 5mg/l BAP, 0.5mg/ l IAA with 30 mg ascorbic acid and 10 mg/l BAP,0.5 mg/l IAA with 30 mg ascorbic acid.Significantly highest number of ischolar_mains and greater ischolar_main length were produced by 0.5 mg/l IAA + 0.5 mg/l IBA.

Keywords

In-vitro:In Glass, NaOCl:Sodium Hypochlorite, BAP:6-Benzyl Amino Purine, IAA:Indole-3-Acetic Acid, IBA:Indole-3-Butyric Acid.

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