International Journal of Plant Protection

Pradnya B. Mane ¹, Pandurang B. Mohite ¹

Affiliations
1 Division of Entomology, Mahatma Phule Krishi Vidyapeeth, College of Agriculture, KOLHAPUR (M.S.), IN

Abstract

The pathogenicity of entomopathogenic fungi, Metarhizium anisopliae (Metsch.) Sorokin, Beauveria brongniartii (Sacc.) and Beauveria bassiana (Balsana) Vuillrmin, was done against white grub, Leucopholis lepidophora (Blanchard) infesting sugarcane under pot culture experiment. In this investigation the virulence of different entomopathogenic fungi were determined by FYM enriched techniques against third instar grubs of Leucopholis lepidophora (Blanch.).An overall concentration range of 2x10⁴-2x10⁸ conidia ml^-1 of entomopathogenic fungi were used but among these, 2x10⁸ conidia ml^-1 concentration was the most promising for highest grub mortality in each entomopathogenic fungi. M. anisopliae was found to be most effective fungus as compared to other fungi. It was observed that 34.49-62.07 per cent grub mortality occurred on 45 DAT in M. anisopliae at different conidial concentrations. B. brongniartii recorded 31.03 to 58.62 per cent grub mortality while, B. bassiana caused 27.59 to 55.18 per cent grub mortality at different conidial concentration. The results showed that M. anisopliae was found to be more pathogenic than B. brongniartii and B. bassiana.

Keywords

Metarhizium anisopliae, Beauveria brongniartii, B. bassiana, Leucopholis lepidophora, Sugarcane.

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Pradnya B. Mane ¹, N. L. Mhase ¹

Affiliations
1 Division of Entomology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN

Abstract

The bioefficacy of different bioagents viz., Paecilomyces lilacinus (2x10⁶ cfu/g), Pseudomonas fluorescens (1x10⁹ cfu/g), Trichoderma viride (2x10⁶ cfu/g), Phule Trichoderma plus (2x10⁶ cfu/g) and Pochonia chlamydosporium (2x106 cfu/g) against second stage juveniles of ischolar_main-knot nematode infesting bottle gourd crop was studied under laboratory conditions. In this bioassay Paecilomyces lilacinus fungus caused higher rate of juvenile mortality at 1.00 per cent concentration was found to be promising in laboratory bioassay studies and recorded 80.00 per cent mortality of second stage juveniles of ischolar_main-knot nematode. This was followed by 76.67 per cent mortality of second stage juveniles of ischolar_main-knot nematode by Pseudomonas fluorescens at 1.00 per cent concentration after 72 hrs treatment of respective bioagents. The fungi Phule Trichoderma plus, Trichoderma viride recorded 70.00, 66.67 per cent juvenile mortality, while as in case of fungi Pochonia chlamydosporium 53.33 per cent juvenile mortality was observed after 72 hrs treatment of respective bioagents at 1.00 per cent concentrations.

Keywords

Meloidogyne incognita, Paecilomyces lilacinus, Pseudomonas fluorescens, Trichoderma viride, Phule trichoderma Plus, Pochonia chlamydosporium, Bottle Gourd.

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