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Totawar, M. V.
- Antibiotic Sensitivity against Xanthomonas axonopodis pv. Citri by Using Hi Media Disc
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Loni, AHMEDNAGAR (M.S.), IN
2 Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, AKOLA (M.S.), IN
1 Department of Plant Pathology, College of Agriculture, Loni, AHMEDNAGAR (M.S.), IN
2 Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, AKOLA (M.S.), IN
Source
International Journal of Plant Protection, Vol 8, No 1 (2015), Pagination: 53-56Abstract
Citrus canker disease is of regular occurrence on several citrus cultivars in varying degrees of incidence depending on the climatic conditions. The bacterium, Xanthomonas causes different symptoms ranging from pustules to necrotic lesions consisting of erumpent corky tissue surrounded by water soaked tissues and yellow halo on leaves, stems and fruits. Sensitivity of different antibiotics were tested against Xanthomonas axonopodis pv. citri isolates (Xac). using eight different Hi-media disc viz., HX006, HX007, HX010, HX032, HX038, HX060, HX067, HX069. Each Hi-media disc having six different antibiotics of different concentration have been tested against Xac isolates. The causal pathogen Xanthomonas axonopodis pv. citri showed sensitivity to most of the antibiotic tested. Hi-media disc HX060 having antibiotic Imepenem (10mg) and Ticarcillin (70mg) showed maximum zone of inhibition (42mm). However, no effect of antibiotics i.e. Co-Trimoxazole, Nofloxacin, Cefoxitin, Erythromycin observed against Xac.Keywords
Xac, Himedia disc, Citrus canker, Antibiotics.
- In Vitro Efficacy of Different Botanicals, Bioagents, Chemicals against Xanthomonas axonopodis Pv.citri by Turbidiometrical Method
Abstract Views :337 |
PDF Views:0
Authors
Affiliations
1 Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN
1 Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN
Source
International Journal of Plant Protection, Vol 8, No 2 (2015), Pagination: 241-244Abstract
Citrus canker disease of acid lime caused by Xanthomonas axonopodis pv. citri is an important disease in many parts of MH region. The bacterium infects the twigs, petioles, fruit stalks and fruits. Action of botanicals, bioagents,chemicals against Xac was studied by turbidiometrically at 24hrs.intervals upto 96hrs by spectrophotometer at 620nm. At 96 hours of incubation least bacterial growth(0.232OD) was exhibited in copperoxychloride + streptomycin sulphate (0.2 % + 200ppm) followed with copper-oxychloride + streptomycin sulphate (0.2% + 100ppm) 0.266 OD statistically superior over all treatments. Similar findings are observed after 24h growth in copper oxychloride + streptomycin sulphate (0.2 % + 200ppm) followed with copper-oxychloride +streptomycin sulphate (0.2 % + 100ppm) (0.303, 0.306, respectively. In botanicals and bioagents neem seed kernel extract (5%) was effective in reducing the growth of bacteria with 0.446 OD at 96 h followed by Pseudomonas flourescence 1×108 cell and Bacillus subtilis 1×108 cell with 0.506 and 0.486 OD, respectively.Keywords
Xac, Citrus Canker, Botanicals, Bioagents, Turbidiometrical Method, Spectrophotometer.References
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