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Manjula, K.
- Survey and Molecular Characterization of Nomuraea Rileyi Isolates
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Authors
K. Swetha
1,
K. Manjula
1
Affiliations
1 Department of Entomology, S.v. Agricultural College, Tirupati (A.P.), IN
1 Department of Entomology, S.v. Agricultural College, Tirupati (A.P.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 482-485Abstract
After conducting a roving survey in Chittoor district for the natural occurrence of Nomuraea rileyi, a few fungal infected and died (mummified) cadavers of Spodoptera litura and Bombyx mori were found and collected. With microscopic studies, the fungus was identified as Nomuraea rileyi and Beauveria bassiana. The molecular characterization of 7 isolates of N.rileyi was done by RAPD-PCR for studying the genetic variability/similarity. RAPD banding profile with 12 different random primers viz., 3 primers from OPD, 3 primers from OPY, 2 primers from OPM, 2 primers from OPA, 1 primer from OPC and 1 primer from OPW (Operon technology) showed 88.6 per cent polymorphism as all the bands obtained were polymorphic with size ranging from 100 bp to 3000 bp. Jaccards similarity co-efficients between the N.rileyi isolates showed 97.50 per cent genetic variation between isolates of Tirupati and V.Kota-1. While the isolates V.Kota-2 and Madanapalli were found to be genetically similar as 70.3 per cent similarity was observed between the isolates. In the resulted dendrogram V. Kota-2 and Madanapalli isolates formed one group and V. Kota-2 and Kanumakupalli another group and remaining isolates did not form any group.Keywords
Nomuraea rileyi, Molecular Characterization, Rapd-pcr, Primers, Polymorphism
- Field Evaluation of Oil formulations of Nomuraea rileyi (Farlow) Samson against Spodoptera litura and Helicoverpa armigera in Groundnut
Abstract Views :211 |
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Authors
T. Sharmila
1,
K. Manjula
1
Affiliations
1 Department of Entomology, S.V. Agricultural College (ANGRAU), TIRUPATI (A.P.), IN
1 Department of Entomology, S.V. Agricultural College (ANGRAU), TIRUPATI (A.P.), IN
Source
International Journal of Plant Protection, Vol 8, No 1 (2015), Pagination: 142-147Abstract
Nomuraea rileyi (Farlow) Samson is a potential entomopathogenic fungus against lepidopteran pests. In groundnut crop ecosystem, along with three oil formulations (sunflower, coconut and groundnut), two Wettable powder (corn flour and talc) and one crude formulation were evaluated against Spodoptera litura and Helicoverpa armigera during 2011-2012 at dry land farm, S.V. Agricultural College, Tirupati. The results indicated that groundnut oil based formulation of N. rileyi recorded significantly higher reduction of both S. litura (82.46%) and H. armigera (74.58%) larval population. The groundnut oil based formulation also recorded significantly lower per cent leaf damage by both S. litura (14.27%) and H. armigera (13.27%). However sunflower oil and coconut oil formulations also proved better than WP and crude formulations. Crude formulation was also found to be effective when compared to WP formulations in reducing the larval population, in recording lower per cent leaves damaged at 15 days after treatment against both pests.Keywords
Oil Formulations, Nomuraea rileyi, Spodoptera litura, Helicoverpa armigera.- Evaluation of Combined Application of Newer Insecticides with Some Fungicides to Control Groundnut Stem Rot, Sclerotium rolfsii sacc. under in Vitro
Abstract Views :333 |
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Authors
P. Venkatarao
1,
K. Manjula
2
Affiliations
1 Department of Agricutural Entomology, Uttar Banga Krishi Viswavidyalaya, Cooch Behar, Pundibari (W.B.), IN
2 Department of Agricutural Entomology, Sri Venkateswara Agricultural College (ANGRAU) Tirupati (A. P.), IN
1 Department of Agricutural Entomology, Uttar Banga Krishi Viswavidyalaya, Cooch Behar, Pundibari (W.B.), IN
2 Department of Agricutural Entomology, Sri Venkateswara Agricultural College (ANGRAU) Tirupati (A. P.), IN
Source
International Journal of Plant Protection, Vol 10, No 1 (2017), Pagination: 34-41Abstract
An in-vitro experiment was conducted to determine the effect of different insecticides, fungicides and their combination treatments on radial colony growth of Sclerotium rolfsii Sacc. by following poison food technique in PDA medium. Two contact (chlorpyriphos 0.05%, thiodicarb 0.075%), three stomach and contact poison insecticides (chlorfenapyr 0.002%, spinosad 0.0025 and emamectin benzoate 0.003%), among the fungicides one systemic (hexaconazole 0.2%), one contact (Mancozeb 0.25%) and their ten insecticide-fungicide combinations were evaluated with recommended doses against S. rolfsii in laboratory during the year 2009-2010 at S.V. Agricultural College, Tirupati. Chlorpyriphos 0.05 per cent (70.33%) was the best insecticide to restrict the fungal growth effectively followed by emamectin benzoate (34.03%). The two fungicides i.e. mancozeb 0.25 per cent, hexaconazole 0.2 per cent and their combinations with insecticides were found effective in reducing (cent per cent) the growth of S. rolfsii. Fungicides do not shown any antagonism when mixed with insecticides.Keywords
Sclerotium rolfsii, Insecticides, Fungicides, Compatibility, in Vitro.References
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