International Journal of Plant Protection

U. K. Kandoliya ¹, D. N. Vakharia ²

Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh Gujarat
2 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh Gujarat, IN

Abstract

Chickpea ( L.) is the most important pulse crop of India. Wilt caused by Fusarium oxysporum f.sp. ciceri is the major limiting factor of chickpea production. The Pseudomonas fluorescens is used as a biocontrol agent against the chickpea wilt pathogen. However, the diversity of such bacteria that can exhibit antagonism against wilt pathogen is poorly exploited. Plant growth-promoting rhizobacterial strains belonging to fluorescent Pseudomonads were isolated from the rhizosphere of chickpea. Ten isolates which exhibited strong antifungal antagonistic activity against Fusarium oxysporum f.sp. ciceri mainly through the production of antifungal metabolites were characterized by PCR-RAPD analysis. A considerable level of molecular diversity was determined among the rhizobacterial isolates of Pseudomonas fluorescens isolated from different chickpea growing areas of Gujarat.

Keywords

Antagonism, Chickpea, Fusarium Oxysporum F.sp. Ciceri, Pseudomonas Fluorescens, Rapd.

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U. K. Kandoliya ¹, D. N. Vakharia ²

Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh Gujarat
2 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh Gujarat, IN

Abstract

Oxalic acid is a simple metabolite produced by many fungi including Fusarium oxysporum f.sp. ciceri. Its phytotoxic activity has been known for years and it has been implicated as a virulence factor for several phytopathogenic fungi. It was also reported that effective reduction of oxalic acid by Pseudomonas fluorescens in a culture medium. So the present experiment has been designed to prove the ability of Pseudomonas fluorescens isolate (Pf-3) as detoxification agent of oxalic acid produced by fungal pathogen in wilt disease condition at various stages of seedling growth in different varieties of chickpea. The result showed the higher oxalic acid content in susceptible variety as compared to resistant one which was positively corresponded to mortality of the seedling due to wilt in wilt sick soil compared to control one. The oxalic acid content was significantly high in a plant grown in wilt sick soil condition in general compared to control one which also reflected higher mortality rate of chickpea seedling particularly in susceptible variety JG-62 compared to rest of the varieties. The significantly lowest value of oxalic acid was observed in Pseudomonas fluorescent Pf-3 seed treated chickpea plants grown even in wilt sick soil also. It indicated effective detoxification of oxalic acid by Pf-3 which induced by Fusarium oxysporum f.sp. ciceri in chickpea seedling. It was also reflected in less mortality of seedlings due to wilt in respective treatment.

Keywords

Chickpea, Fusarium Oxysporum F.sp. Ciceri, Wilt, Oxalic Acid, Pseudomonas Fluorescens

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U. K. Kandoliya ¹, D. N. Vakharia ¹

Affiliations
1 Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, JUNAGADH (GUJARAT), IN

Abstract

An induction of defense mechanisms against pathogens along with induction of antioxidant system is the mechanisms by which PGPR promote plant growth promotion is well known. So, the present experiment was conducted to study the plant defense system induced by PGPR bacteria Pseudomonas fluorescens against Fusarium wilt in chickpea. The results suggest that seed treatment of Pseudomonas fluorescens effective elicits activity of defense-related antioxidant system such as ascorbic acid and APX leading to improved plant resistance and reduces the mortality of chickpea plant against soil borne disease like wilt.

Keywords

Ascorbic Acid, Ascorbate Peroxidase, Chickpea, Fusarium oxysporum f.sp. Ciceri, Pseudomonas fluorescens Isolates.

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