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Pathak, D. M.
- In vitro Evaluation of Fungicides and Phytoextracts Against Alternaria tenuissima -An Incitant of Pigeonpea [Cajanus cajan (l.) Millsp.] Leaf Blight
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Authors
K. A. Parmar
1,
D. M. Pathak
1
Affiliations
1 Department of Plant Pathology, College of Agriculture, Navsari Agricultural University,Campus Bharuch, Maktampur (Gujarat), IN
1 Department of Plant Pathology, College of Agriculture, Navsari Agricultural University,Campus Bharuch, Maktampur (Gujarat), IN
Source
International Journal of Plant Protection, Vol 12, No 2 (2019), Pagination: 94-97Abstract
Pigeonpea leaf blight caused by Alternaria tenuissima is one of the most important foliar disease causing heavy losses under South Gujarat region. The present investigation was carried out to evaluate the inhibitory activity of different fungicides and phytoextracts against A. tenuissima and to find out the efficient fungicide and phytoextract at preliminary level. The experiment conducted in the P.G. Research Laboratory, Department of Plant Pathology, N.M.C.A., Navsari Agricultural University, Navsari. Different fungicides and phytoextracts were evaluated against the growth of A. tenuissima by using poisoned food technique. Among nine fungicides tested, cent per cent growth inhibition was observed in hexaconazole 4% + zineb 68%, propiconazole and tebuconazole at 500ppm and 1000ppm. Among all nine phytoextracts tested, lowest mycelial growth (21.00mm) was recorded in garlic leaf extract at 10 per cent with highest per cent growth inhibition (74.28 PGI).Keywords
Alternaria tenuissima.
References
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- In vitro Antifungal Activity of Plant Extracts (Sterilized and Unsterilized) against Macrophomina phaseolina (Tassi) Goid. Cause Stem Canker of Pigeonpea [Cajanus Cajan (l.) Millsp.]
Abstract Views :309 |
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Authors
Mulji Jehani
1,
D. M. Pathak
1
Affiliations
1 Department of Plant Pathology, College of Agriculture, Navsari Agricultural University,Campus Bharuch, Maktampur (Gujarat), IN
1 Department of Plant Pathology, College of Agriculture, Navsari Agricultural University,Campus Bharuch, Maktampur (Gujarat), IN
Source
International Journal of Plant Protection, Vol 12, No 2 (2019), Pagination: 105-109Abstract
The present paper reports the antifungal activity of plant extracts (5 % Concentration) from seven plant species viz., Bougainvillea spectabilis, Zingiber officinali, Datura stramonium, Curcuma longa, Ocimum sanctum, Hibiscus bombycideron, Tegetes erecta. Antifungal activity was tested against Macrophomina phaseolina. All plant extracts (sterilized and unsterilized) exhibited considerable distinction in radial mycelial growth of tested pathogen. Overall, Curcuma longa appeared significantly the most effective and suppressed the radial mycelial growth (sterilized plant extract, 47.13% and unsterilized plant extract, 48.28 %) of the Macrophomina phaseolina. However, Ocimum sanctum exhibited minimum inhibition (sterilized plant extract, 25.29 % and unsterilized plant extract, 22.99 %) against Macrophomina phaseolina. It may be concluded from the present investigation that Curcuma longa can be utilized for the management of Macrophomina phaseolina.Keywords
Stem Canker, In vitro, Antifungal, Plant Extracts.References
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- Nene, Y.L., Sheila, V.K. and Sharma, S.B. (1984). A world list of chickpea and pigeonpea pathogens. 5th Edn., Patancheru 502 324, Andhra Pradesh, India: International Crops Research Institute for Semi-Arid Tropics.
- Vincent, J.M. (1947). Distortion of fungal hyphae in presence of certain inhibitors. Nature, 150: 850.
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- In vitro Antagonistic Activity of Trichoderma Species against Important Soil Borne Pathogens
Abstract Views :285 |
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, NAU, Campus Bharuch, Maktampur (Gujarat), IN
1 Department of Plant Pathology, College of Agriculture, NAU, Campus Bharuch, Maktampur (Gujarat), IN
Source
International Journal of Plant Protection, Vol 12, No 2 (2019), Pagination: 127-131Abstract
The rhizospheric soil samples were collected from different cultivated agricultural fields from Bharuch and Narmada districts and the mycoflora were isolated by serial dilution plate technique. Total eight isolates of Trichoderma viride, Trichoderma harzianum and Trichoderma longibrachiatum were isolated on Potato Dextrose Agar medium. The green coloured colonies were identified by comparing with taxonomic key. They were purified by single spore isolation method and maintained on PDA slants at 40C in the refrigerator at Department of Pl.Pathology, NMCA, NAU, Navsari. Soil borne Pathogenic fungi viz., Sclerotium rolfsii, Macrophomina phaseolina and Fusarium oxysporum were isolated from the respective diseased plants during field survey in Navsari Agricultural University farm, Navsari. The antagonistic efficacy against test pathogen was evaluated by dual culture plate technique. Among all 8 Trichoderma isolates, The Trichoderma harzianum NCJD8 isolate has showed 24.17 mm mycelial growth with 73.15 per cent inhibition of Sclerotium rolfsii, where in case of Macrophomina phaseolina, Minimum mycelial growth (32.67 mm) of test pathogen was recorded in T. longibracheatum NCJD2 isolate with 63.70 per cent inhibition which was statistically at par with T. viride NCJD6 (34.50 mm) with 61.67 per cent inhibition and when it comes to Fusarium oxysporum, T. harzianum NCJD5 showed minimum mycelial growth and highest per cent growth inhibition (75.56%) with 22.00 mm colony diameter of the pathogen after seven days of incubation which was statistically at par with isolate T. harzianum NCJD1 (72.96%) with 24.33 mm colony diameter.Keywords
Antagonistic Activity, Soil Borne Pathogens.References
- Aneja, K.R. (2003). Experiments in Microbiology, Plant Pathology and Biotechnology, 4th ed., New Age International (P) Ltd.
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- Gurha, S.N. (2001). Effect of some Trichoderma spp. on the growth of different isolates of Fusarium oxysporum f.sp. ciceri in vitro. Annals Plant Protec. Sci., 9(2) : 332-334.
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- Madhusudan, P., Gopal, K., Haritha, V., Sangale, U.R. and Rao, S.V.R.K. (2010). Compatability of Trichoderma viride with fungicides and efficiency against Fusarium solani. J. Plant Dis. Sci., 5 : 23-26.
- Pan, S. and Bhagat, S. (2007). Antagonistic potential of Trichoderma and Gliocladium spp. from West Bengal. J. Mycology & Plant Pathol., 37(2) : 235-243.
- Vishwanath, K., Gopal, K. and Gopi, V. (2008). Isolation of potential Trichoderma spp. Associated with dry ischolar_main rot infected acid lime (Citrus aurantifolia Swingle). J. Plant Disease Sci., 3 (2) : 165-168.
- In vitro Evaluation of Fungicides and Organic Extracts against Macrophomina phaseolina (Tassi) Goid. Isolated from Pigeonpea [Cajanus cajan (l.) Millsp.]
Abstract Views :331 |
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Authors
D. M. Pathak
1,
R. R. Patel
1
Affiliations
1 Department of Plant Pathology, College of Agriculture, Navsari Agricultural University,Campus Bharuch, Maktampur (Gujarat), IN
1 Department of Plant Pathology, College of Agriculture, Navsari Agricultural University,Campus Bharuch, Maktampur (Gujarat), IN
Source
International Journal of Plant Protection, Vol 12, No 2 (2019), Pagination: 147-151Abstract
Ten fungicides at three different concentrations were screened in vitro by poisoned food technique for evaluating their efficacy against M. phaseolina, in which mancozeb (dithane M-45, 75% WP), carbendanzim (bavistin, 50% WP), carbendanzim + mancozeb (sixer, 75%WP) and metalexyl 18% + mancozeb 64% (ridomil, 75% WP) were proved to be highly toxic to the growth of the M. phaseolina. Eight different organic extracts were tested against M. phaseolina by poisoned food technique with different concentration in vitro. All the extracts were inhibitory to M. phaseolina significantly lower mycelium growth was recorded in Neem cake followed by coconut. Next best were FYM, mustard cake, sesamum cake and vermicompost. While lowest inhibition of mycelial growth of M. phaseolina was observed in groundnut cake and castor cake.Keywords
Fungicides, Pigeonpea, Organic Extracts.References
- Agrawal, S.C. (1993). Disease of green gram and black gram international book distributors, pp. 1-2, 5, 28, 32-33, 159, 181, 269.
- Chavan, R.V. (2006). Investigation on ischolar_main rot (Macrophomina phaseolina (Tassi) Goid of cotton (Gossypium herbaceum L.). M.Sc. (Ag.) Thesis, Junagadh Agricultural University, Junagadh (Gujarat) India.
- Dhingani, C.J. (2011). Management of ischolar_main rot disease (Macrophomina phaseolina (Tassi) Goid. of chickpea (Cicer arietinum L.) under south gujarat conditions. M.Sc. (Ag.) Thesis, Navsari Agril. University, Navsari (Gujarat) India.
- Jaiman, R.K., Jain, S.C. and Sharma, P. (2009). Field evaluation of fungicides, bioagents and soil amendments against ischolar_main rot caused by Macrophomina phaseolina in cluster bean. J. Mycol. Pl. Pathol., 39(1): 74-76.
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- Lambhate, S.S., Chaudhari, G.K., Mehetre, S.S. and Zanjare, S.R. (2002). In vitro evaluations of chemicals against ischolar_main rot of cotton caused by M. phaseolina. J. Maharashtra Agril. Uni., 21(1): 99-100.
- Nakarni, N.R. (1991). Investigation on Macrophomina phaseolina (Tassi.) Goid., causing ischolar_main rot of castor. M.Sc. (Ag.) Thesis, Gujarat Agricultural University, S.K. Nagar (Gujarat) India.
- Naz, F., Rauf, C.A., Haque, I.U. and Ahmad, I. (2006). Management of Rhizoctonia solani with plant diffusates and chemicals. Pak. J. Phytopathol., 18(1): 36-43.
- Nene, Y.L., Sheila, V.K. and Sharma, S.B. (1984). A world list of chickpea and pigeonpea pathogens. 5th Edn., Patancheru 502 324, Andhra Pradesh., India: International Crops Research Institute for Semi-Arid Tropics.
- Nene, Y.L., Sheila, V.K. and Sharma, S.B. (1989). A world list of chickpea (Cicer arirtinum L.) and Pathology progress Report-7. Patancheru, A. P., India: ICRISAT, pp. 23.
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