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Banana (Musa paradisiaca L.) is the most popular tropical fruit crops grown in India. It is affected by several post-harvest diseases among them anthracnose (Colletotrichum gloeosporioides (P. Henn), caused huge loss in quality and quantity of fruits. Therefore affords were made to screen the different systemic, contact and combination of six fungicides, botaniclas and biocontrol agents in vitro condition against test fungus. Among systemic fungicides, carbendazim 50 per cent WP at 50 ppm, contact fungicides copper oxychloride 50 per cent WP at 500 ppm and combination of fungicides carbendazim 12 per cent WP + mancozeb 63 per cent WP at 100 ppm concentrations were found the most effective and gave cent per cent growth inhibition of C. gloeosporioides. Lantana leaves extract at 10 per cent solvent was also effective and resulted 66.41 per cent growth inhibition of C. gloeosporioides. Among the biocontrol agents, Trichoderma harzianum gave 62.43 per cent growth inhibition of C. gloeosporioides (62.43%).

Keywords

Banana, Antharcnose, Colletotrichum gloeosporioides, Trichoderma harzianum in Vitro Evaluation of Fungicides, Botanicals.

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References

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Effect of Culture Media and Temperature on Growth and Sporulation of Colletotrichum lindemuthianum of Urdbean in vitro

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Authors

M. J. Sardhara ¹, D. K. Davara ², A. M. Moradia ², H. J. Kapadiya ³

Affiliations
1 Department of Plant Pathology, Junagadh Agricultural University, Junagadh (Karnataka), IN
2 Main Oilseeds Research Station, Junagadh Agricultural University, Junagadh (Karnataka), IN
3 Main Oilseeds Research Station, Junagadh Agricultural University, Junagadh (Karnataka), IN

Source

International Journal of Plant Protection, Vol 9, No 1 (2016), Pagination: 47-51

Abstract

Anthracnose (Colletotrichum lindemuthianum) fungus is an important in the urdbean legumes crop. In the present investigation, the rate of growth of Anthracnose (C. lindemuthianum) has been compared in various temperature, culture media solid and liquid while in case solid media types viz., simple PDA (Potato Dextrose Agar), Potato carrot sucrose agar, Carrot juice agar, Blackgram leaf extract agar, Richard's agar, Czapeck's (Dox) agar, Asthana and Hawker's agar had tested. Among all the solid media tested, maximum mycelial growth was obtained in Potato dextrose agar medium (80.04 mm), which was statistically at par with Richard's agar medium (76.52 mm). Similarly in the liquid media, maximum dry mycelial weight was recorded in Richard's broth medium (444.34 mg) after 10 days of inoculation which was significantly superior over rest of the broth medias and followed potato dextrose agar broth (429.03 mg). Temperature plays an important role in infection and disease development. Maximum mean colony diameter of fungus was recorded at temperatures of 30°C (80.92 mm) and 25°C (78.62 mm) which was significantly superior over all other temperatures. Lowest mean colony diameter was obtained at temperatures of 10° (24.01 mm) and 40°C (21.37 mm). This is important for further study of disease management.

Keywords

Blackgram (Vigna mungo L. Hepper), Culture Media, Temperature.

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