Refine your search
Collections
Co-Authors
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Ramesh, S.
- AFLP Analysis of Genetic Relationships and Diversity of Some Oriental Pickling Melon (Cucumis melo Var. conomon) Genotypes in Karnataka
Abstract Views :314 |
PDF Views:0
Authors
Affiliations
1 Department of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Directorate of Education, University of Horticultural Sciences, Udyanagiri, Bagalkot (Karnataka), IN
3 Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
1 Department of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
2 Directorate of Education, University of Horticultural Sciences, Udyanagiri, Bagalkot (Karnataka), IN
3 Department of Genetics and Plant Breeding, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 2 (2017), Pagination: 242-248Abstract
Genetic diversity of indigenous plant population is the prerequisite for any research on plant breeding and improvement, making genetic diversity conservation of critical importance. The present study was conducted to assess the amplified fragment length polymorphism (AFLP) marker based diversity in 15 oriental pickling melon (Cucumis melo var. conomon) genotypes during 2009 at University of Agricultural Sciences (UAS), Bangalore. Thirteen primer combinations generated a total of 443 amplicons, of which 342 were polymorphic (76%) with an average of 26.31 bands. The primer combination E-CTT/M-GTG was found to amplify 45 scorable bands, with 91.11 per cent polymorphic bands. Cluster analysis based on Jaccard similarity index and UPGMA algorithm showed a high variation within the studied genotypes and a mean similarity index of 0.79 for AFLP markers. The genotypes CMC GKVK 1 and CMC GKVK 6 expressed the least similarity (0.627) and may serves as very good source in melon breeding programme. Genotypes CMC GKVK1, CMC GKVK8, CMC GKVK15, CMC GKVK11 and CMC GKVK6 fell into solitary clusters and these accessions could be used in future genetic, physiological and morphological studies.Keywords
Oriental Pickling Melon, AFLP, Genetic Diversity.
References
- Behera, T.K., Gaikward, A.B., Singh, A.K. and Staub, J.E. (2008). Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). J. Sci. Food Agric., 88 :733-737.
- Benbouza, H., Jacquemin, J.M., Baudoin, J.P. and Mergeai, G. (2006). Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels, Biotechnol. Agron. Soc. Environ, 10: 77-81.
- Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for sample quantities of fresh leaf tissue. Phytochem. Bul., 191:11-15.
- Garcia, M.J., Oliver, M.H., Gomez, P.H. and Devicente, M.C. (2000). Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor. Appl. Genet., 101: 860-864.
- Given, D.R., Bran,Well D., Haman, O., Heywood, V. and Synge, H. (1987). What the conservationist requires of ex situ collections. Botanic Gardens and the World Conservation Strategy. Academic Press, London , 103-116.
- Jaccard, P. (1908). Nouvelles recherches sur la distribution florale, Bull. Soc. Vaud. Sci. Nat., 44 : 223-270.
- Lombard, V., Baril, C.P. , Dubreuil, P. F. Blouet and Zhang, D. (2000). Genetic relationship and finger printing of rapeseed cultivars by AFLP: Consequences for varietal registration. Crop Sci. 40:1417-1425.
- Luan, F., Sheng, Y., Wang, Y. and Staub, J.E. (2010). Performance of melon hybrids derived from parents of diverse geographic origins. Euphytica., 173 :1-16.
- Maras, M.J., Sustar-Vozlic, B. Javornik and Meglic, V. (2008). The efficiency of AFLP and SSR markers in genetic diversity estimation and gene pool classification of common bean (Phaseolus vulgaris L.). Acta Agric. Slovenica., 91:87-96.
- Padmavathi, N., Tomason, Y. R., Jeong, J., Gopinath, V., Levi, A., Gibson, P. and Reddy, U.K. (2008). Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites. Plant Genetic Resources: Characterizat. & Utilizat., 7(2): 127-134.
- Park, Y.H., Kim, K.H., Choi, Y.M., Choi, H.S., Chae, Y., Park, K.S. and Chung, S.M. (2010). Evaluation of TYLCV- resistant tomato germplasm using molecular markers. Kor. J. Hort. Sci. Technol., 28: 89-97.
- Raghami, M., Zamani, Z., Lopez-Sese, A.I., Hasandokht, M.R., Moghadam Fattahi, M.R. and Kashi, A. (2014). Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers. Plant Syst. Evol., 300:139-151.
- Ramanatha, R.V. and Hodgkin, T. (2002). Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell Tissue & Organ Cult., 68:1-19.
- Reddy, O., Tomason, Y.R., Zlenko, I., Bashet, A., Kaur, P., Levi, A., Bates, G.T. and Nimmakayala, P. (2005). Molecular diversity in ukrainian melon germplasm. Congr. Plant Animal Genomes. Proceed. Plant Animal Genomes, 13: 177.
- Rohlf, F.J. (2000). NTSYS-pc: Numerical taxonomy and multivariate analysis system, version 2.1, Exeter Publishing, Setauket, NEW YORK, U.S.A.
- Sekhon, M.S. and Gupta, V.P. (1995). Genetic distance and heterosis in Indian mustard: developmental isozymes as indicator of genetic relationships. Theo. Appl. Genet., 91:1148-1154.
- Sheng, Y.Y., Wang, Y.H. and Luan, F.S. (2011).Distribution of simple sequence repeat and AFLP marker on melon genetic map1. China Veget., 1 (8) : 39-45.
- Vos, P., Hogers, R., Bleeker, M., Reijang, M., Van De Lee, T., Hornes, M., Frijters, A., Plot, J., Kuiper, M. and Zabeau, M. (1995). AFLP a new technique for DNA finger printing. Nucleic Acids Res., 23 : 4407-4444.
- Xu, Z., Hu, T. and Zhang, F. (2012). Genetic diversity of walnut revealed by AFLP and RAPD markers. J. Agric. Sci., 4 : 271-276.
- Yashiro, K., Hirouoshi, I., Yukari, A. and Ken,O.T. (2005). Genetic relationships among Asian melon (Cucumis melo L.) Revealed by AFLP analysis. Breed. Sci., 55:197-206.
- Identification of High Yielding and Blast Disease Resistant F6 RILs in Finger Millet
Abstract Views :183 |
PDF Views:0
Authors
Affiliations
1 Department of Genetics and Plant Breeding, University of Agricultural Sciences (G.K.V.K.), Bengaluru (Karnataka), IN
2 AICRP, University of Agricultural Sciences (G.K.V.K.), Bengaluru (Karnataka), IN
3 Zonal Agricultural Research Station, VC Farm, Mandya (Karnataka), IN
1 Department of Genetics and Plant Breeding, University of Agricultural Sciences (G.K.V.K.), Bengaluru (Karnataka), IN
2 AICRP, University of Agricultural Sciences (G.K.V.K.), Bengaluru (Karnataka), IN
3 Zonal Agricultural Research Station, VC Farm, Mandya (Karnataka), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 2 (2017), Pagination: 338-347Abstract
Finger millet [Eleusine coracana (L.) Gaertn.] is one of the most important staple food crops in India. Blast disease caused by the fungus Pyricularia grisea (Cooke) is the most devastating biotic production constraint which affects different aerial parts of the plant at all plant growth stages. Development of pure-line varieties with high grain yield potential coupled with blast disease resistance is the major breeding objective of breeding finger millet. 360 F6 Recombinant Inbred Lines (RILs) derived from the cross PR 202×GPU 48 were evaluated at two locations during 2015 rainy season (Bengaluru and Mandya) for grain yield and response to blast disease reaction. Analysis of variance in F6 RILs at both Bengaluru and Mandya locations revealed highly significant mean squares attributable to ‘RILs’ and ‘check varieties’ for all traits studied. High GCV and PCV were observed for grain yield plant-1, neck blast incidence and finger blast incidence at Bengaluru and Mandya locations. All the traits studied exhibited higher broad sense heritability for both locations. The best ten high yielding RILs were identified.Keywords
Recombinant Inbred Lines, Grain Yield, Blast, Variability.References
- Angadi, C., Mohan Rao, A., Vijayakumar, K.V., Ramesh, S., Gowda, M.V.C., Nagaraja, A. and Gopinath Rao, M. (2016). Identification of high yielding and blast disease resistant genotypes in F4 and F5 generations of finger millet [Eleusine coracana (L.)Gaertn].Environ. Ecol.,34(4C): 2386-2395.
- Barbeau, W.E. and Hilu, K.W. (1993). Protein, calcium, iron and amino acid content of selected wild and domesticated cultivars of finger millet. Plant Foods Hum. Nutr., 43: 97-104.
- Bedis, M.R., Ganvir, B.N. and Patil, P.P. (2006). Genetic variability in finger millet. J. Maharastra Agric. Univ., 31(3): 369-370.
- Dhanalaxmi, T.N. (2009). Dissection of variability for grain macro and micronutrients contents, morpho-metric traits and their inter-relationship in finger millet (Eleusine coracana (L.) Gaertn.] core and mini core germplasm accessions. M.Sc. (Ag.) Thesis, University of Agricultural Sciences, Bengaluru, KARNATAKA (INDIA).
- Federer, W.T. (1956). Augmented (or hoonuiaku) designs. Hawaiian Planters’ Record LV, 2 : 191-208.
- Fisher, R.A., Immer, F.R. and Tedin, O. (1932). The genetical interpretation of statistics of the third degree in the study of quantitative inheritance. Genetics, 17(2): 107.
- Ganapathy, S., Nirmalakumari, A. and Muthiah, A.R. (2011). Genetic variability and inter relationship analyses form economic traits in finger millet germplasm. World J. Agric. Sci., 7 (2): 185-188.
- Govindu, H.C., Shivanandappa, N. and Renfro, B.L. (1970). Observations on diseases of Eleusine coracana with special reference to host resistance to the helminthosporium disease. Indian Phytopathology Society, New Delhi. Plant Disease Problems, pp.415-424.
- Hanson, C.H., Robinson, H.F. and Comstock, R.E. (1956). Biometrical studies of yield in segregating population of Korean Lespedeza. Agron.J., 48 : 267-282.
- Hulbert, S.H., Webb, C.A., Smith, S.M. and Sun, Q. (2001). Resistance gene complexes: Evolution and utilization. Annu. Rev. Phytopathol., 39 : 285-312.
- John, K. (2006).Variability and correlation studies in quantitative traits of finger millet (Eleusine coracana Gaertn.). Agric. Sci. Digest., 26(3): 166-169.
- Johnson, H.W., Robinson, H.F. and Comstock, R.E. (1955). Estimates of genetic and environmental variability in soybean. Agron. J., 47 : 314-318.
- Kiran, Babu T., Thakur, R.P., Upadhayaya, H.D., Reddy, P.N., Sharma, R., Girish, A.G. and Sarma, N.D.R.K. (2013). Resistance to blast (Magnaporthe grisea) in a mini-core collection of finger millet germplasm. European J. Plant Pathol.,135(2): 299-311.
- Leung, W., Busson, F. and Jardin, C. (1968). Food composition table for use in Africa. FAO, Rome, Italy. pp. 306.
- Nandini, B., Ravishankar, C.R., Mahesha, B., Shailaja, H. and Kalyana, M.K.N. (2010). Study of correlation and path analysis in F2 population of finger millet. Internat. J. Plant Sci., 5(2): 602-605.
- Patro, T.S.S.K. and Madhuri, J. (2014). Identification of resistant varieties of finger milletfor leaf, neck and finger blast. Internat. J. Food Agric. Veter. Sci., 4(2): 7-11.
- Ravikumar, R.L. and Seetharam, A. (1993). Character association in segregating populations of finger millet (Eleusine coracana G.) in blast epidemic region. Indian J. Agric. Sci.,63(2): 96-99.
- Reddy, V.G., Upadhyaya, H.D., Gowda, C.L.L. and Singh, S. (2009).Characterization of Eastern African finger millet germplasm for qualitative characters at ICRISAT.J. SAT Agric. Res., 7: 1-9.
- Robson, D.S. (1956). Application of K4 statistics to genetic variance component analysis.Biometrics, 12: 433-444.
- Rossman, A.Y., Howard, R.J. and Valent, B. (1990). Pyricularia grisea the correct name for the rice blast disease fungus Mycologia, 82: 509-512.
- Satish, D. (2003). Studies on genetic diversity based on productivity and variability for quality traits in finger millet (Eleusine coracana L. Gaertn.). M.Sc. (Ag.) Thesis, University of Agricultural Sciences, Dharwad, KARNATAKA (INDIA).
- Snedecor, G.W. and Cochran, W.G. (1994). Statistical methods.8th Ed. IOWA State University Press, Ames, IOWA, USA.
- Sonnad, K.S., Shanthakumar, G. and Salimath, P.M. (2007). Genetic variability and character association studies in white ragi (E. coracana Gaertn). Karnataka J. Agric. Sci., 21(4): 572-575.
- Viswanath, S., Sanne Gowda, S., Seetharam, A. and Shankare Gowda, B.T. (1986). Reaction to blast disease of released and pre-released varieties of finger millet from different states. Millet Newsl., 5: 31.
- Anonymous (2015). Ministry of Agriculture and farmers welfare, GOI, http://agricoop.nic.in/ReportsPublication.aspx.