International Journal of Agricultural Sciences

Harshal E. Patil ¹, S. B. Thawari ¹, Ravikiran S. Mali ¹

Affiliations
1 Seed Monitoring Cell, Directorate of Research Office (Dr. Panjabrao Deshmukh Krishi Vidyapeeth) Akola, M.S., IN

Abstract

Molecular characterization and genetic diversity of seven CMS, seven maintainers and four restorer lines of cotton (Gossypium spp.) was investigated through Randomly Amplified Polymorphic DNA markers. The similarity indices showing the evolutionary similarity varied from a minimum of 0.36 to maximum of 0.90. In case of CMS and maintainers lines, the pairs viz., 1 A and 1 B, 3 A and 3 B, 7A and 7 B exhibited maximum genetic similarities which revealed that these genotype are genetically less divergent or genetically more closure than any other genotypes. The maximum genetic similarity was observed between restorers, RHC 054 and RHC 058 (0.90) followed by RHC 035 and RHC 056 (0.80). The dendrogram tree constructed using Win Boot computational analysis showed two completely distinct groups. The CMS and maintainer pairs viz., 1 A and 1 B, 2 A and 2 B, 3 A and 3 B, 5 A and 5 B, 9 A and 9 B formed a separate cluster, whereas 7 A and 7 B, 10 A and 10 B formed a second distinct cluster. Each CMS and its maintainer line revealed homology with each other. The dendrogram obtained from restorers showed two distinct clusters. Cluster one consisting of RHC 035 and RHC 0056 while, second cluster consisting of RHC 054 and RHC 058. The results revealed that considerable amount of diversity is present among CMS, maintainers and restorer lines of cotton and each genotype is also characteristically indentified by their RAPD marker profile. Moreover, genetic diversity was not only related to the geographic diversity but also natural and artificial selection, exchange of breeding material and genetic drift.

Keywords

Genetic Diversity, Rapd Markers, Cotton, Molecular Characterization

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Harshal E. Patil ¹, Ravikiran S. Mali ¹, Avdhut R. Giri ¹, Sachin B. Thawari ¹

Affiliations
1 Department of Agricultural Botany, Seed Monitoring Cell, Directorate of Research, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, M.S., IN

Abstract

In forty genotypes of niger (Guizotia abyssinica Cass) substantial genetic variability was observed for nine characters studied. Heritability estimate was low for primary branches/plant, moderate for seed yield/plant and high for remaining all characters. Significant positive and higher genotypic correlation of seed yield with seeds/capitulum, days to flower and days to maturity was observed; while it was moderate with 1000 seed weight and plant height. A positive phenotypic as well as genotypic correlation among days to flower, days to maturity, seeds/capitulum and 1000 seed weight and their positive association with seed yield was observed, indicating that these were the major yield components in niger. However, on the basis of correlation and path analysis, days to maturity, seeds/capitulum and 1000 seed weight were the major yield components. Since late maturity beyond limit is not desirable, greater emphasis should be laid on seeds/capitulum and 1000 seed weight. Hence, strategies for genetic improvement by using study of variability, correlation and path analysis in niger have been strongly adopted for further crop improvement.

Keywords

Niger, Crop Improvement, Variability, Path Analysis And Correlation

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