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Sathyanarayana, B. N.
- Optimization of Explants Density for Tissue Culture Propagation of Banana cv. 'GRANDE NAINE'
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Authors
Affiliations
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), IN
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), IN
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 1 (2017), Pagination: 71-76Abstract
Cost of production is always stressed as the main obstacle for tissue culture. Nutrient media is one of the most costly input which accounts for 30-35 per cent of total cost of tissue culture propagation. Production cost, therefore, can be reduced by efficient utilization of culture media. To find out optimum quantity of media required for shoot proliferation, 1-5 multiple bud explants were incubated in each culture bottle. Explants density of 4/culture bottles was found best as it recorded higher total shoot production/l (291.94), shoot length (3.22 cm), number of leaves/shoot (2.81) and lower cost per shoot (Rs. 1.175). In vitro ischolar_maining was carried out with densities of 6, 8, 10 and 12 microshoots/culture bottle. The maximum response with regard to ischolar_maining had not yet been reached as there were no significant differences among the treatments. Incubation of 4 multiple bud explants and 12 microshoots per culture bottles is optimum for scaling-up of tissue culture production of banana cv. 'GRANDE NAINE'.Keywords
Tissue Culture, Explants Density, Grande Naine, MS Medium, Cost/Shoot.
References
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- Cheaper Carbon Sources for Micropropagation of Banana cv. 'GRANDE NAINE'
Abstract Views :205 |
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Authors
Affiliations
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), IN
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
1 Center for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot (Karnataka), IN
2 Division of Horticulture, University of Agricultural Sciences, G.K.V.K., Bengaluru (Karnataka), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 1 (2017), Pagination: 124-131Abstract
Micropropagation of banana has become a routine procedure but high production cost is limiting the commercial use of tissue culture technology. Analytical grade sucrose is the most commonly used carbon source for the micropropagation banana, however, the cost is too high to justify the use at commercial scale. Therefore, inexpensive and readily available sources of carbon such as laboratory grade sucrose, common grade sugar, cube sugar, rock sugar, candy sugar, glucose, jaggery and sugarcane juice were evaluated for in vitro propagation of banana cv. 'GRANDE NAINE'. Best response in terms of shoot multiplication and ischolar_maining were achieved with rock sugar and common grade sugar, respectively which could be compared well with that of analytical grade sucrose. The results showed the possibility of successful use of cheaper carbon sources for micropropagation of banana cv. 'GRANDE NAINE'.Keywords
Analytical Grade Sucrose, Common Grade Sugar, Grande Naine, Micropropagation, Rock Sugar.References
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