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Kumar, Anil
- Effect of Different Dose of Cytokinin for Shoot Multiplication of Banana (Musa paradisiaca L.) Variety ‘GRAND NAINE’ under In-vitro Condition
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Authors
Yogesh Prasad Rajbhar
1,
Manmohan Singh
1,
Anil Kumar
1,
Gopal Singh
1,
Abhimanyu Singh
1,
D. K. Singh
1
Affiliations
1 Sardar Vallabhbhai Patel University of Agriculture Technology, Meerut (U.P.), IN
1 Sardar Vallabhbhai Patel University of Agriculture Technology, Meerut (U.P.), IN
Source
International Journal of Agricultural Sciences, Vol 12, No 1 (2016), Pagination: 65-72Abstract
The maximum callus formation (20.3%) was observed in treatment BAP 8 mgl-1 while the minimum (4.6%) was noted under control. With the combination of BAP and BA, the maximum callus formation ( 27.0%) was recorded under BAP 8 mgl-1 + BA 4 mgl-1 ; however, it was at par with BAP 8 mgl-1 + BA 3 mgl-1 at 75 days after inoculation.At 90 days after inoculation, maximum callus percentage (29.3) was found under BAP 8 mgl-1+ BA 4 mgl-1. At 105 days after inoculation, callus percentage was maximum callus (33.0%) was noted under the treatment combination of BAP 8 mgl-1+ BA 4 mgl-1, however, it was significantly at par with BAP 8 mgl-1+ BA 3 mgl-1, while the minimum (9.3%) was recorded under control again. The earliest shoot initiation (21.0, 22.0 days, respectively) was noted under BAP 8 mgl-1 and BA 5 mgl-1, separately; while it was statistically earliest i.e. 20.66 days in combination with BAP 2 mgl-1+ BA 5 mgl-1. Maximum shoot length (0.76 cm) was recorded in the treatment of BAP 8 mgl-1. at 20 days after shoot initiation.Maximum shoot length (3.06 cm) was noted under BAP 8 mgl-1 alone which statistically superior to other under BAP alone treatments while it recorded minimum under control at 40 days after shoot initiation. Under BA treatments, the maximum shoot length (2.26 cm) was noted with BA 4 mgl-1 and 5 mgl-1 both; however, it was at par with BA 2 mgl-1 and 3 mgl-1 at 40 days after shoot initiation. With the effect of BAP and BA combinations, the maximum shoot length (3.23cm) was recorded under BAP 8 mgl-1+ BA 5 mgl-1 at 40 days after shoot initiation. The minimum duration of ischolar_main initiation (14.66 days) was noted under the treatment of Indole Butyric acid 4 mgl-1 ; however, it was significantly at par with indole butyric acid 2 mgl-1 and 3 mgl-1. The maximum duration (34.33 days) was observed under control. Minimum number of ischolar_mains (4.0 ischolar_mains) were recorded under the treatment applied 1 mgl-1 IBA in culture medium. Further, number of ischolar_mains was found maximum 10.33 ischolar_mains under the treatment of 5 mgl-1 IBA followed by 4 mgl-1 IBA concentrations. Culture medium with IBA 5 mgl-1 showed maximum ischolar_main length (1.66 cm) followed by IBA 4 mgl-1 , 3 mgl-1 and 2 mgl-1 with 1.56 cm, 1.40 cm and 1.06 cm, respectively. It was concluded that BAP 8 mgl-1 and BA 5 mgl-1 separately performed better results on account of callus formation, shoot initiation and multiplication of shoots whereas with their combination viz., BAP 8 mgl-1 +BA 4 mgl-1 showed best result on the above parameters. For ischolar_main initiation and its development IBA 5 mgl-1 was found to be the best among all the treatments.Keywords
Cytokinin, Shoot Multiplication, Banana, In-vitro Condition.
References
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- Effect of Organic Manures, Biofertilizers and Micronutrients on Growth, Yield and Quality of Onion (Allium cepa L.)
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Authors
Anil Kumar
1,
R. B. Ram
1,
Sutanu Maji
1,
Sachin Kishor
1,
Rahul Yadav
1,
Govind
1,
Kamal Ram Meena
1
Affiliations
1 Department of Applied Plant Science (Horticulture), Babasaheb Bhimrao Ambedkar University, Lucknow (U.P.), IN
1 Department of Applied Plant Science (Horticulture), Babasaheb Bhimrao Ambedkar University, Lucknow (U.P.), IN
Source
International Journal of Agricultural Sciences, Vol 13, No 2 (2017), Pagination: 236-241Abstract
A field experiment was conducted during the Rabi season to find out the effect of organic manures, biofertilizers and micronutrients on growth, yield and quality of onion cv. NHRDF Red - 2. There were 13 treatments viz., T0 (Recommended dose of fertilizers), T1 Poultry manure, T2 vermicompost, T3 Azotobacter, T4 VAM, T5 Azotobacter+RDF (50%)+zinc, T6 Azotobacter+RDF(75%)+zinc, T7 VAM+RDF(50%)+boron, T8 VAM+RDF (75%)+boron, T9 RDF (25%)+VAM+poultry manure (50%)+Azotobacter+boron, T10 (RDF (25%)+VAM+vermicompost (50%)+Azotobacter+boron, T11 RDF (25%)+VAM+poultry manure (50%) Azotobacter+zinc, T12 RDF (25%)+VAM+vermicompost 50% Azotobacter+zinc and the experiment was laid out under RBD with three replications. The study clearly revealed that there were significant effects of various treatments on the growth, yield and quality attributes of onion. The number of leaves per plant (12.15), plant height (73.02cm), neck thickness (22.00mm), bulb length (6.46 cm), bulb diameter (7.20cm), yield (398.36 kg/ha-1) were recorded maximum in treatment T12 whereas T.S.S (14 °B), vitamin C (12.11mg/100g), total sugars (10.52%), reducing sugar (6.23%) and non-reducing sugar (4.28%) were found maximum in T10 treatment as compared to other treatment. However, T12 was good for higher yield improvement and T10 was the best for quality improvement among the all treatments under study, the application of T12 (RDF (25%)+VAM+Vermicompost 50% Azotobacter+Zinc) may be suggested for successful cultivation of onion in Lucknow.Keywords
Organics, Biofertilizers, Micronutrients, Onion, Yield, Quality.References
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- Kumar, Sandeep, Maji, Sutanu, Kumar, Sanjay and Singh, Harsh Deep (2014). Efficiency of organic manures on growth and yield of radish (Raphanus sativus) cv. JAPANESE White. Internat. J. Plant. Sci., 9 (1) : 57-60.
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- Sahu, Ashish Kumar, Kumar, Sanjay and Maji, Sutanu (2014). Effect of biofertilizers and inorganic fertilizers on vegetative growth and yield of okra [Abelmoschus esculentus (L.) Moench]. Internat. J. Agric. Sci., 10 (2) : 558-561.
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- Screening of Okra (Abelmoschus esculentus) Cultivars for Resistant Against Root-Knot Nematode, Meloidogyne incognita
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Authors
Affiliations
1 Department of Nematology, C.C.S. Haryana Agricultural University, Hisar (Haryana), IN
1 Department of Nematology, C.C.S. Haryana Agricultural University, Hisar (Haryana), IN
Source
International Journal of Agricultural Sciences, Vol 16, No 2 (2020), Pagination: 203-207Abstract
The ischolar_main-knot nematode (Meloidogyne incognita) is one of the major limiting factors affecting plant growth and yield causing an estimated $100 billion loss per year worldwide. Synthetic pesticides, though instantaneously effective, are usually prohibitively expensive, not readily available, may cause hazards to both man and livestock and inflict injury to the environment. Notable among the alternatives to nematicides, use of resistant cultivars which are inexpensive and eco-friendly is the only and effective option available for its control. In the present studies, seventy one okra (Abelmoschus esculentus) cultivars were evaluated for resistant against M. incognita under screen house conditions. One week old okra plants of test cultivars were inoculated with 1000 freshly hatched second stage juveniles of M. incognita. Results clearly revealed that all the cultivars showed varying degree of susceptibility against M. incognita. One cultivar (EC 306703) showed resistant and seven cultivars (EC 306697, EC 306700, EC 359891, EC 305718, IC 014018, BB-1, Hisar naveen and Hisar unnat) were rated as moderately resistant reaction against M. incognita. Rest of the cultivars exhibited either susceptible or highly susceptible reaction against M. incognita. No any single cultivar has been found as highly resistant. Cultivar, EC 306703 was rated as resistant and showed less damage by nematode as compared to susceptible cultivars and their planting could provide a useful tool to control ischolar_main-knot nematode.Keywords
Meloidogyne incognita, Okra, Resistant, Screening, Cultivars.References
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- Mohanta, S. and Mohanty, K.C. (2012). Screening of okra germplasms/varieties for resistance against Meloidogyne incognita. J.Plant Protec. & Environ., 9: 66-68.
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- Evaluation of Different Rice Genotypes for Resistant Against Rice Root-Knot Nematode, Meloidogyne graminicola
Abstract Views :357 |
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Authors
Affiliations
1 Department of Nematology, C.C.S. Haryana Agricultural University, Hisar (Haryana), IN
1 Department of Nematology, C.C.S. Haryana Agricultural University, Hisar (Haryana), IN
Source
International Journal of Agricultural Sciences, Vol 16, No 2 (2020), Pagination: 249-253Abstract
Screening of different rice genotypes were tested for their resistant reaction against rice ischolar_main-knot nematode,Meloidogyne graminicola. In the present studies, 79 rice genotypes/lines (44 genotypes from OG series and 35 genotypes from AR series including Pusa 1121 and TN-1 as susceptible checks) were evaluated for resistant reaction against M. graminicola was carried out under screen house, Department of Nematology, CCS Haryana Agricultural University, Hisar, Haryana during Kharif, 2019-20. Seeds of each genotype were sown in the earthen pots (1 kg soil capacity) containing steam sterilized sandy loam soil. One week old seedlings of rice genotypes were inoculated with freshly hatched second stage juveniles of M. graminicola @ 2000 J2/pot. Forty five days after inoculation, observations were recorded such as number of eggs and second stage juveniles. The result reveals that the genotypes showed great variation in reaction to M. graminicola from resistant to highly susceptible reaction. Out of 44 genotypes from OG series, 34 showed resistant reaction. However, two genotypes (OG-4 and OG-37) were found moderately resistant and remaining was categorized as susceptible reaction. Similarly, out of 35 genotypes from AR series, two genotypes (AR-08, AR-31) showed resistant reaction against M. graminicola. Four genotypes (AR-06, AR-20, AR-21 and AR-32) showed moderately resistant reaction and rests of the genotypes were categorized as susceptible reaction against M. graminicola. Most of the genotypes were found resistant/moderately resistant to M. graminicola which can be used for future breeding programmes to develop resistant reaction in these genotypes.Keywords
Meloidogyne graminicola, Rice, Resistant, Screening, Genotypes.References
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- Rao, M., Grithlahre, S., Bisen, P., Singh, N.K., Dar, M.H., Singh, U.S. and Singh, P.K. (2016).Genetics of marker assisted backcross progenies of the cross HUR-105 X Swarna-SUB1. Internat. J. Agric., Environ. & Biotechnol., 9: 499-505.
- Ravindra, H., Sehgal, M., Narasimhamurthy, H.B., Khan, H.S.I. and Shruthi, S.A. (2015). Evaluation of rice landraces against rice ischolar_main-knot nematode, Meloidogyne graminicola. African J. Microbiology Research, 9: 1128-1131.
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