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Namita,
- Optimising Protocol for Direct Differentiation of Shoot Buds From Leaf ex-plant of Tagetes erecta L. Var. Pusa Narangi Gainda
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1 Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, IN
1 Division of Floriculture and Landscaping ICAR- Indian Agricultural Research Institute, New Delhi, IN
Source
International Journal of Agricultural Sciences, Vol 16, No 2 (2020), Pagination: 238-243Abstract
Marigold is an important loose flower crop has gained popularity for its pharmaceutical, industrial, medicinal and therapeutic importance. In this present study, we developed a protocol for direct regeneration using in-vitro raised immature leaves of African marigold (Tagetes erecta L.var. “Pusa Narangi Gainda”). A total of eight treatment combinations of growth hormones such as BAP, IAA and GA3with MS as a basal media was studied. The best medium found for direct, shoot organogenesis from leaf ex-plant was treatment (T3) - MS + BAP 0.5 mg/l + IAA 0.25 mg/l. Pre-treatment with Carbendazim (0.2 %) + Mancozeb (0.2 %) + 8-HQC (200 mg/l) for 2hr followed by HgCl2 for 4 min. resulted in minimum fungal (24.33%), bacterial (8.33%) contamination simultaneously it increased the survival percentage upto (67.33%). This study is helpful for rapid clonal propagation, production of lutein-rich pharmaceutical compounds and secondary metabolites using tissue culture techniques.Keywords
African Marigold, Shoot Organogenesis, Leaf Ex-plant, Growth Hormones, Clonal Propagation.
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