Identification of Bacterial Isolates, PCR Amplification and In-Silico Analysis of Nitroreductase and Rubrerythrin Responsive Genes from Shilajit

16S rRNA gene sequence homology in combination with morphological, physiological and biochemical characteristics was used as the tools for identification of the selected isolates from Shilajit samples designated EUS1, EUS2 and EUS3 which were collected from mountains of Baru Sahib, Sirmour district of Himachal Pradesh. The isolated strains were identified as B. subtilis, B. cereus,B. clausii and were deposited in GenBank with accession number KP126518, KP126519, and KP126520 respectively. Furthermore, the present study was designed to analyze molecular modeling of proteins from Bacillus spp. (KP126518: BsNR, KP126519: BcRbr) and was carried out by subjecting to in silico analysis. Motif scan analysis revealed the presence of signature sequences that might be related to structure and enzymatic function. The 3D structure of Bacillus proteins were predicted by swiss-model server based on homology modeling method. The modeled structures were validated by various validation servers. Six probable active sites in B. subtilis (BsNR) and two in B. cereus (BcRbr) were predicted for target protein structure.