Molecular Characterization of Native Trichodermaharzianum Sp. and Evaluation of Different Biocontrol Agents under in Vitro and in Vivo Conditions against Stem Rot and Wilt Disease of Carnation

Different fungal antagonists viz., Trichodermaharzianum, T. hamatum, T. viride, T. polysporum,T. virens, Penicilliumsp., Aspergillussp., Rhizopus sp. and two bacterial antagonistic species namely Bacillus subtilis and Pseudomonas fluorescens were evaluated under in vitro and field conditions against stem rot of carnation and seven microorganisms representing five fungal viz., T. harzianum, T. viride, T. virens, Aspergillus sp. and Penicillium sp. and two bacteria viz., Bacillus sp. and bacterial isolate 1 were tested against Fusarium wilt of carnation during 2012 and 2013. Under in vitro conditions biocontrol agents significantly inhibited the growth and under field conditions they caused significant reduction in disease incidence and there is increase plant growth and quality parameters as compared to untreated control. The Trichodermaharzianum was found most effective and identity of T.harzianum were also established by PCR amplification and sequencing of either 18S rRNA or ITS gene sequences and it was submitted with an accession number KF924562.