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Age Related Histological Changes of Human Spleen


Affiliations
1 Dept. of Anatomy, Gauhati Medical College, Guwahati, Assam, India
2 Jorhat Medical College, India
3 Gauhati Medical College, Guwahati, Assam, India
 

The spleen was a source of intrigue to ancient physician and philosophers. It is the largest lymphoid organ takes important part in body immunity, as high incidences of serious bacterial infections were reported following splenectomy in infancy. A research work was carried out in the department of Anatomy, Gauhati Medical College. The histological characteristics of 21 normal human spleens were studied under light microscope in different age groups and to correlate them functionally. The specimen of spleen varies from newborn to eighty year old cadavers, within stipulated time limit after fulfilling the formalities. The spleens were first washed in normal saline, dried with blotting paper. Tissues were fixed, processed and slides were prepared using standard laboratory procedure of haematoxylin and eosin staining. The size of white pulp that seen on histological sections, increase with age, reach its peak around puberty and then involutes. Sizes of white pulp were measured and then statistically analysed using Student's T-test. P value d" 0.05 is considered as statistically significant. The data obtained in this study will help in certain medico-legal practices and will give insight of different clinical presentations. This study may be used as a podium for further sophisticated studies.

Keywords

Human Spleen, White Pulp, Red Pulp, Marginal Zone.
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  • Carleton HM, Short RHD. The spleen. Schaffer’s Essentials of Histology. 6th ed. New York. Toronto: Longman green and co. London; 1956. p. 56-87.

  • Bloom & Fawcett. The spleen. A Textbook of Histology. 9th ed. Toranto: W.B Saunders; 1970. p. 487-500.

  • Brunicardi F. C, Andersen D. K. Schwartz’s Principles and Practice of Surgery. 8th ed. New Delhi: Mc Graw-Hill Medical Publishing division; 2005. p. 1298.

  • Standring S. Spleen. Gray’s Anatomy-The Anatomical Basis of Clinical Practice. 40th ed. Churchill Livingstone: Elsevier; 2008. p. 1191-1194.

  • Young B, James S, Lowe, Stevens A, Health J. W. Wheater’s Functional Histology-A text and colour atlas. 5th ed. Sriniwaspuri, New Delhi: Churchill Livingstone; 2007: p. 229-233.

  • Ullah M. The Spleen, Circulatory system, Histology and Genetics, Theoretical and Applied. 1st ed. Meerut: Kedarnath Ram Nath Publisher; 1978. p. 480-485.

  • Wilfred M. C. Bailey’s Textbook of Histology. 3rd ed. Baltimore 2, USA: The William and Wilkins Company; 1975. p. 353-363.

  • Ham A. W & Cormack D. H. Histology. 8th ed. Philadelphia and Toronto: JB Lippincott company; 1979. p. 356-358.

  • C Ronald Lesson, Thomas S. Lesson, Anthony A. Papario. Text Book of Histology. 5th ed. Philadelphia: W. B. Saunders Company; 1985. p. 277-278.

  • Boyd W. A Textbook of Pathology- structure and function in disease. Varghese Publishing House; 1988. p. 1157-60.

  • Kiernander B. Physical medicine in Paediatrics. 3rd ed. London Butterworth: W.B. Saunders Company; 1965. p. 8. Vol 1.

  • Bosker G. Geriatrics Emergency Medicine. 2nd ed. New York. Mosby year book; 1990. p. 286. Vol 1.

  • Grosfeld JL, O’Neill JA. Paediatric surgery. 6th ed. New Delhi. Mosby publisher; 2006. p. 1691. Vol 2.


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  • Age Related Histological Changes of Human Spleen

Abstract Views: 209  |  PDF Views: 82

Authors

Himamoni Deka
Dept. of Anatomy, Gauhati Medical College, Guwahati, Assam, India
T. K. Das
Jorhat Medical College, India
K. L. Talukdar
Gauhati Medical College, Guwahati, Assam, India

Abstract


The spleen was a source of intrigue to ancient physician and philosophers. It is the largest lymphoid organ takes important part in body immunity, as high incidences of serious bacterial infections were reported following splenectomy in infancy. A research work was carried out in the department of Anatomy, Gauhati Medical College. The histological characteristics of 21 normal human spleens were studied under light microscope in different age groups and to correlate them functionally. The specimen of spleen varies from newborn to eighty year old cadavers, within stipulated time limit after fulfilling the formalities. The spleens were first washed in normal saline, dried with blotting paper. Tissues were fixed, processed and slides were prepared using standard laboratory procedure of haematoxylin and eosin staining. The size of white pulp that seen on histological sections, increase with age, reach its peak around puberty and then involutes. Sizes of white pulp were measured and then statistically analysed using Student's T-test. P value d" 0.05 is considered as statistically significant. The data obtained in this study will help in certain medico-legal practices and will give insight of different clinical presentations. This study may be used as a podium for further sophisticated studies.

Keywords


Human Spleen, White Pulp, Red Pulp, Marginal Zone.

References