Journal of Horticultural Sciences

S. Raghavendra ¹, C. K. Ramesh ², V. Kumar ³, M. H. M. Khan ⁴, B. S. Harish ⁵

Affiliations
1 Department of Biochemistry, College of Horticulture, University of Horticultural Sciences, Bagalkot – 587102, IN
2 Department of Studies in Biotechnology, Sahyadri Science College, Shivamogga – 577 102, Karnataka, IN
3 Department of Biochemistry, Davangere University (Kuvempu University P.G Center), Shivagangothri, Davangere - 577 002, Karnataka, IN
4 Department of Chemistry, Jawaharlal Nehru National College of Engineering, Shivamogga – 577201, Karnataka, IN
5 Department of Medicinal and Aromatic plants, College of Horticulture Sciences, University of Horticulture Sciences, Bagalkot – 587102, Karnataka, IN

Abstract

The genus Mucuna is an important medicinal herb and is extensively used in traditional Indian systems of medicine for various ailments. In vitro culture technique provides an alternative to plant propagation and germplasm conservation. Our aim was to study the biochemical changes occurring during regeneration of shoots (plantlets) from explants of two accessions of Mucuna pruriens, by monitoring the efficiency of nitrogen utilization and changes in levels of some hydrolytic enzymes. A rapid micropropagation system was developed using Murashige and Skoog's (MS) medium supplemented with BAP and IAA combined. In both the accessions, 3.0mg l^-1 6-BAP, in combination with 0.2mg l^-1 IAA, induced shoot buds and shoot elongation; however for multiple-shoot induction, a slightly higher concentration of cytokinin, i.e., 3.5mg l^-1 6-BAP, in combination with 0.2mg l^-1 IAA, was required. Results of the present study confirm an active growth of explants revealed by nitrate assimilation enzymes and hydrolytic enzymes. It is concluded that medium composition, growth regulator combination and culture incubation conditions are all vital in both the accessions of Mucuna pruriens for induction of in vitro plant regeneration.

Keywords

Mucuna, in vitro, Biochemical Changes, Regeneration, Enzymes.

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M. M. Mustaffa ¹, V. Kumar ¹

Affiliations
1 National Research Centre for Banana, Tiruchirapalli- 620 102, IN

Abstract

Bananas and plantains globally the fourth important food crop, recorded wide variation in production and productivity in most of the banana growing regions. This is attributed mainly to the variety, type of planting material used, season and method of planting besides management techniques such as water and nutrients. Among all the commercial varieties of banana, owing to comparatively higher yield potential and better marketability both in domestic and export markets the Cavendish group of bananas such as Grand Naine, Williams, Robusta are preferred over other cultivars of banana. Planting sword suckers is beneficial and more ideal conventional planting material than the water suckers, butts and bits of rhizomes. However, the in-vitro banana plants of Dwarf Cavendish and Robusta are superior to conventional suckers due to their vigorous growth, early flowering (19 days) and reduced crop duration by 29 and 22 days, respectively. With reference to plant spacing and planting density which is determined by varieties grown, soil fertility status, prevailing climatic conditions etc. and maintenance of lower density of 625-1000 plants ha^-1 recorded low productivity at <30t ha^-1, while, high density of 5000 plants ha^-1 recorded 120t ha^-1. However, time taken for maturity was distinctly longer in higher densities, with 120 and 160 days Bunch grade and fruit quality are, however determined by pre-harvest bunch management practices. Influence of method of irrigation, fertilizer application and the role of nutrient elements, organic farming, INM and use of bunch sleeves on yield and fruit quality in different commercial cultivars under various conditions is discussed.

Keywords

Bananas, Planting Density, Drip and Fertigation, Nutrition, Bunch Sleeves.

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