Journal of Horticultural Sciences

S. R. Singh ¹, A. S. Sundouri ¹, M. K. Sharma ¹, K. K. Srivastava ¹, H. A. Dar ¹

Affiliations
1 Division of Pomology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, IN

Abstract

Murashige and Skoog's medium (MS) and Woody plant (WP) medium supplemented with IBA and BAP at different concentrations were tested for culture proliferation in sour cherry. MS medium supplemented with 1 mg L^-1 BAP + 0.1 mg L^-1 IBA, and, Woody plant medium fortified with 2 mg L^-1 BAP + 0.1 mg L^-1 IBA recorded maximum proliferation efficiency. MS medium supplemented with BAP @ 0.1 mg L^-1 and devoid of auxin recorded optimum elongation of micro-shoots. MS medium fortified with 2 mg L^-1 IBA gave highest ischolar_maining percentage and average number of ischolar_mains per explant. BAP and IBA in MS medium were ideal for proliferation indices like number of shoots per explant, average length of shoots (mm) and percentage of shoots with desired length for ischolar_maining, and, can be used in a multiplication programme.

Keywords

Cherry, MS Medium, Woody Plant Medium (WPM), Indole–3-Butyric Acid, Benzylaminopurine.

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S. R. Singh ¹, N. Ahamed ¹, K. K. Srivastava ¹, D. Kumar ¹, S. Yousuf ¹

Affiliations
1 ICAR-Central Institute of Temperate Horticulture, Old Air Field Rangregth, Srinagar - 190 007, Jammu and Kashmir, IN

Abstract

To assess the nature and magnitude of genetic diversity in long day onion germplasm by using the principal component analysis and single linkage cluster analysis an experiment was carried out with 34 onion genotypes. High coefficient of variation with wide range in traits indicated an appreciable variability in germplasm. Genotypes were classified into seven principal components having Eigen value > 1, cumulatively accounted for 83.87% of total variability. Principal Component - I contributed for 24.73% of total variation for followed by principal component-II (15.27%). PC-I had high positive loading for bulb weight (0.401), marketable yield (0.338), total bulb yield (0.401) and PC-II had high positive loading for plant height (0.412), PC-III for high T.S.S. (0.276) PC -IV for A grade bulbs (0.436), PC-V for polar diameter of bulbs (0.514), PC-IV negatively loaded with purple blotch (-0.461) and PC-VII for narrow neck thickness (-0.515). Plotting PC-I aganist PC-II differenciated CITH-O-13, CITH-O-4, CITH-O-22, CITH-O-19, CITH-O-9, CITH-O-6 and CITH-O2 as most divergent genotype.On the basis of single linkage cluster means cluster-I was most importent for average bulb weight, minimum bolters, high marketble bulb percentage high marketable and total bulb yield whereas cluster -II was important for maximum nuber of leaves/plant and minimum neck thicknes. Highest inter-cluster distance was observed between cluter II and Cluster-I(873.5%).Most divergent genotypes with high inter cluter distance could be the most appropriate parents for crop impovement in onion.

Keywords

Genetic Diversity, Onion, Principal Component Analysis, Single Linkage Cluster Analysis.

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