Journal of Horticultural Sciences

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Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity


Affiliations
1 Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, India
2 Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, India
3 Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, India
4 Department of Biochemistry, Kuvempu University, Shankaraghatta-577 451, Karnataka, India
 

Vitamins are essential nutrients in food crucial for maintaining good health. Tomato, being a widely consumed vegetable, provides a good quantity of vitamins. Metabolite profiling of vitamins at different stages of fruit maturity in a crop helps identify the right stage for better quality. Based on preliminary screening for quality parameters, tomato lines rich in TSS, antioxidants, lycopene and beta-carotene were selected for the present study. Eight genotypes and a wild species were profiled for 'B' vitamins at three different stages of fruit maturity, viz., green, breaker and ripe stage. A simple and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of six 'B' vitamins was developed and validated by us. Among the genotypes studied, IIHR-249-1 recorded higher niacin, pantothenic acid and biotin content. Pyridoxine content was higher in the hybrid, Arka Rakshak. The wild species, LA-1777(Solanum habrochaites) was found to be rich in pantothenic acid, riboflavin and thiamine. Content of most of the vitamins increased with ripening of the fruit. IIHR-249-1 and LA-1777 were found to be rich in 'B' vitamins, earlier reported to be also rich in antioxidants and lycopene. These genotypes can be used for improving the nutritive value of tomato under crop improvement programmes, through conventional breeding or biotechnological approaches.

Keywords

Tomato, B Vitamins, LC-MS/MS-MRM, Fruit Ripening, Green Stage, Breaker Stage.
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  • Metabolite Profiling for Six 'B' Vitamins Using LC-MS in Tomato Genotypes at Different Stages of Fruit Maturity

Abstract Views: 202  |  PDF Views: 131

Authors

P. Kavitha
Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, India
K. S. Shivashankara
Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, India
T. K. Roy
Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, India
K. C. Pavithra
Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, India
V. K. Rao
Division of Plant Physiology and Biochemistry, ICAR-Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru –560 089, India
A. T. Sadashiva
Division of Vegetable Crops, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, India
K. V. Ravishankar
Division of Biotechnology, ICAR-Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru-560 089, India
G. J. Sathish
Department of Biochemistry, Kuvempu University, Shankaraghatta-577 451, Karnataka, India

Abstract


Vitamins are essential nutrients in food crucial for maintaining good health. Tomato, being a widely consumed vegetable, provides a good quantity of vitamins. Metabolite profiling of vitamins at different stages of fruit maturity in a crop helps identify the right stage for better quality. Based on preliminary screening for quality parameters, tomato lines rich in TSS, antioxidants, lycopene and beta-carotene were selected for the present study. Eight genotypes and a wild species were profiled for 'B' vitamins at three different stages of fruit maturity, viz., green, breaker and ripe stage. A simple and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for simultaneous determination of six 'B' vitamins was developed and validated by us. Among the genotypes studied, IIHR-249-1 recorded higher niacin, pantothenic acid and biotin content. Pyridoxine content was higher in the hybrid, Arka Rakshak. The wild species, LA-1777(Solanum habrochaites) was found to be rich in pantothenic acid, riboflavin and thiamine. Content of most of the vitamins increased with ripening of the fruit. IIHR-249-1 and LA-1777 were found to be rich in 'B' vitamins, earlier reported to be also rich in antioxidants and lycopene. These genotypes can be used for improving the nutritive value of tomato under crop improvement programmes, through conventional breeding or biotechnological approaches.

Keywords


Tomato, B Vitamins, LC-MS/MS-MRM, Fruit Ripening, Green Stage, Breaker Stage.

References