Himachal Journal of Agricultural Research

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Ascochyta rabiei Infections Modify Expression of Chickpea Invertase Genes Differentially in Contrasting Genotypes


Affiliations
1 Department of Agricultural Biotechnology, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India
 

Sucrose is the main form of assimilated carbon and energy source in plants. Sucrose in sink tissues is hydrolyzed by invertases to glucose and fructose that act as major carbon molecules for plant metabolism. Initial studies in some crops suggested role of invertases in plant disease resistance however, no information on these genes is available for chickpea biotic stresses. To identify the role of invertases in resistance/susceptibility to ascochyta blight (causal organism: Ascochyta rabiei) in chickpea, expression of six invertase genes (two cell wall invertases, one vacuolar invertase and three alkaline/neutral invertases) was evaluated in A. rabiei infected susceptible (GPF2) and resistant (HC1) genotypes of chickpea. Of these six gene, only one overexpressed in susceptible GPF2 whereas in resistant HC1 five genes overexpressed. The study suggested that down regulation of invertase genes was associated with susceptibility of chickpea to A. rabiei whereas over expression was associated with resistance.

Keywords

Ascochyta blight, Cicer arietinum, cell wall invertase, vacuolar invertase, alkaline/neutral invertase, gene expression, Ascochyta rabiei
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  • Chen Z, Gao K, Su X, Rao P and An X. 2015. Genome-wide identification of the invertase gene family in Populus. PloS One 10 (9): e0138540.

  • Essmann J, Schmitz-Thom I, Schon H, Sonnewald S, Weis E and Scharte J.2008. RNA interference-mediated repression of cell wall invertase impairs defense in source leaves of tobacco. Plant Physiology 14 (3): 1288-1299.

  • Gaur RB and Singh RD. 1996. Effects of Ascochyta blight on grain yield and protein in chickpea. Indian Journal of Mycology and Plant Pathology 26: 259-262.

  • Gomez-Ariza J, Campo S, Rufat M, Estopa M, Messeguer J, Segundo BS and Coca M. 2007. Sucrose-mediated priming of plant defense responses and broad-spectrum disease resistance by overexpression of the maize pathogenesis-related PRms protein in rice plants. Molecular Plant–Microbe Interactions 20 (7): 832-842.

  • Kiran A, Sharma PN, Awasthi R, Nayyar H, Seth R, Chandel SS, Siddique KHM, Zinta G and Sharma KD. 2021.

  • Disruption of carbohydrate and proline metabolism in anthers under low temperature causes pollen sterility in chickpea. Environmental and Experimental Botany188: 104500.

  • Kocal N, Sonnewald U and Sonnewald S. 2008. Cell wall bound invertase limits sucrose export and is involved in symptom deve lopment and inhibi t ion of photosynthesis during compatible interaction between tomato and Xanthomonas campestris pv vesicatoria. Plant Physiology 148 (3): 1523-1536.

  • Livak KJ and Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-””CT method. Methods 25 (4): 402-408.

  • Long DE, Fung AK, McGee EEM, Cooke RC and Lewis DH. 1975. The activity of invertase and its relevance to the accumulation of storage polysaccharides in leaves infected by biotrophic fungi. New Phytologist 74 (2): 173-182.

  • Moghaddam MRB and Ende V. 2012. Sugars and plant innate immunity. Journal of Experimental Botany 63 (11): 3989-3998.

  • Nonis A, Ruperti B, Pierasco A, Canaguier A, AdamBlondon AF, Di Gaspero G and Vizzotto G. 2008. Neutral invertases in grapevine and comparative analysis with Arabidopsis, poplar and rice. Planta 229 (1): 129-142.

  • Parrent JL, James TY, Vasaitis R and Taylor AF. 2009. Friend or foe? Evolutionary history of glycoside hydrolase family 32 genes encoding for sucrolytic activity in fungi and its implications for plant-fungal symbioses. BMC Evolutionary Biology 9 (1): 1-16.

  • Roitsch T, Balibrea ME, Hofmann M, Proels R and Sinha AK. 2003. Extracellular invertase: key metabolic enzyme and PR protein. Journal of Experimental Botany 54 (382): 513-524.

  • Roitsch T and Gonzaez MC. 2004. Function and regulation of plant invertases: sweet sensations. Trends in Plant Science 9 (12): 606-613.

  • Sharma KD, Patil G and Kiran A. 2021. Characterization and differential expression of sucrose and starch metabolism genes in contrasting chickpea (Cicer arietinum L.) genotypes under low temperature. Journal of Genetics. 100:71.

  • Siemens J, Gonzalez MC, Wolf S, Hofmann C, Greiner S, Du Y, Rausch T, Roitsch T and Muller L. 2011. Extracellular invertase is involved in the regulation of clubroot disease in Arabidopsis thaliana. Molecular Plant Pathology 12 (3): 247-262.

  • Swarbrick PJ, Schulze-Lefert P and Scholes JD. 2006. Metabolic consequences of susceptibility and resistance (race-specific and broad-spectrum) in barley leaves challenged with powdery mildew. Plant, Cell & Environment 29 (6): 1061-1076.

  • Tang X, Rolfe SA and Scholes JD. 1996. The effect of Albugo candida (white blister rust) on the photosynthetic and carbohydrate metabolism of leaves of Arabidopsis thaliana. Plant, Cell & Environment 19 (8): 967-975.

  • Tauzin AS and Giardina T. 2014. Sucrose and invertases, a part of the plant defense response to the biotic stresses. Frontiers in Plant Science 5:293.

  • Taylor SC, Nadeau K, Abbasi M, Lachance C, Nguyen M and Fenrich J. 2019. The ultimate qPCR experiment: producing publication quality, reproducible data the first time. Trends in Biotechnology 37 (7): 761-774.

  • Yao Y, Geng MT, Wu XH, Liu J, Li RM, Hu XW and Guo JC. 2015. Genome-wide identification, expression, and activity analysis of alkaline/neutral invertase gene family from cassava (Manihot esculenta Crantz). Plant Molecular Biology Reporter 33 (2): 304-315.


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  • Ascochyta rabiei Infections Modify Expression of Chickpea Invertase Genes Differentially in Contrasting Genotypes

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Authors

Bharti Shree
Department of Agricultural Biotechnology, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India
Rajeev Rathour
Department of Agricultural Biotechnology, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India
Kamal Dev Sharma
Department of Agricultural Biotechnology, College of Agriculture CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, India

Abstract


Sucrose is the main form of assimilated carbon and energy source in plants. Sucrose in sink tissues is hydrolyzed by invertases to glucose and fructose that act as major carbon molecules for plant metabolism. Initial studies in some crops suggested role of invertases in plant disease resistance however, no information on these genes is available for chickpea biotic stresses. To identify the role of invertases in resistance/susceptibility to ascochyta blight (causal organism: Ascochyta rabiei) in chickpea, expression of six invertase genes (two cell wall invertases, one vacuolar invertase and three alkaline/neutral invertases) was evaluated in A. rabiei infected susceptible (GPF2) and resistant (HC1) genotypes of chickpea. Of these six gene, only one overexpressed in susceptible GPF2 whereas in resistant HC1 five genes overexpressed. The study suggested that down regulation of invertase genes was associated with susceptibility of chickpea to A. rabiei whereas over expression was associated with resistance.

Keywords


Ascochyta blight, Cicer arietinum, cell wall invertase, vacuolar invertase, alkaline/neutral invertase, gene expression, Ascochyta rabiei

References