Himachal Journal of Agricultural Research

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Nucleotide Sequence Variation in Exons 9 and 10 of Lactoferrin Gene in Murrah Buffalo


Affiliations
1 Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal - 132 001, India
 

The study was undertaken with the objectives to characterize and identify single nucleotide polymorphisms in exons 9 and 10 of lactoferrin gene in Murrah buffalo. Genomic DNA was isolated from 200 lactating Murrah buffaloes and exons 9 and 10 of lactoferrin gene were amplified using specific sets of primers, which resulted in amplicons of 332 and 209 bp respectively. Comparison of nucleotide sequence of exonic region of lactoferrin gene in Murrah buffalo with that of Bos taurus cattle revealed a total of 16 mutations comprising of 11 transition and 5 transversion type. Conceptualized translation of nucleotide sequence of exonic region revealed six amino acid changes (five in exon 9 and one in exon 10). The BLAST analysis revealed that exons 9 and 10 of lactoferrin gene in Murrah buffalo were 94 to 99 % identical with other species. The identified SNPs may serve as marker for selection of Murrah buffaloes for improved resistance to mastitis.

Keywords

Lactoferrin, Murrah Buffalo, SNP.
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  • Nucleotide Sequence Variation in Exons 9 and 10 of Lactoferrin Gene in Murrah Buffalo

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Authors

Dinesh Krishanender
Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal - 132 001, India
Archana Verma
Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal - 132 001, India
I. D. Gupta
Molecular Genetics Lab, Animal Genetics and Breeding Division, ICAR-National Dairy Research Institute, Karnal - 132 001, India

Abstract


The study was undertaken with the objectives to characterize and identify single nucleotide polymorphisms in exons 9 and 10 of lactoferrin gene in Murrah buffalo. Genomic DNA was isolated from 200 lactating Murrah buffaloes and exons 9 and 10 of lactoferrin gene were amplified using specific sets of primers, which resulted in amplicons of 332 and 209 bp respectively. Comparison of nucleotide sequence of exonic region of lactoferrin gene in Murrah buffalo with that of Bos taurus cattle revealed a total of 16 mutations comprising of 11 transition and 5 transversion type. Conceptualized translation of nucleotide sequence of exonic region revealed six amino acid changes (five in exon 9 and one in exon 10). The BLAST analysis revealed that exons 9 and 10 of lactoferrin gene in Murrah buffalo were 94 to 99 % identical with other species. The identified SNPs may serve as marker for selection of Murrah buffaloes for improved resistance to mastitis.

Keywords


Lactoferrin, Murrah Buffalo, SNP.

References