Himachal Journal of Agricultural Research

Open Access Open Access  Restricted Access Subscription Access

Crossability Among Backcross Progenies Harbouring Different Resistant Genes for Rice Blast and Bacterial Blight Diseases and Elite Genotype HPR 2143 of Rice


Affiliations
1 Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
2 Department of Seed Science and Technology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
 

Rice blast and bacterial leaf blight are the two major diseases affecting rice productivity in north-western Himalayan region of India. Exploitation and utilization of major resistance (R) genes is an effective way to control these diseases. The selected elite BC2F2 progenies of four crosses, HPR2143/DHMAS164, HPR2143/PB1, HPR2143/IRBB54 and HPR2143/PR114 were used as a source for blast resistance genes Pita and Pi9, and bacterial leaf blight resistance genes Xa21 and Xa38 respectively. In order to pyramid 2 genes for each disease, hybridization among these backcross derivatives containing single genes was attempted. Overall 346 spikelets were emasculated and pollinated to combine genes, Pita and Pi9, out of which 136 seeds were set with a mean crossability of 39.31 per cent. Similarly to combine genes, Xa21 and Xa38, 335 spikelets were emasculated and pollinated in which 127 seeds were set with an overall crossability of 37.91 per cent. Overall crossability over both sets of crosses (Pita × Pi9) and (Xa21 × Xa38) was recorded to be 38.61 per cent that was comparatively low as compared to earlier documented reports.

Keywords

Rice, Hybridization, Pita, Pi9, Xa21, Xa38.
User
Notifications
Font Size

  • Akhtar Sabina, Bhat MA, Wani Shafiq A, Bhat KA, Chalkoo S, Mir MR and Wani Shabir A. 2010. Marker assisted selection in rice. Journal of Phytology 2 (10): 66-81.

  • Azzini LE and Rutger JN. 1982. Amount of outcrossing on different male steriles of rice. Crop Science 22: 905-907.

  • Brar DS and Khush GS. 1997. Alien introgression in rice. Plant Molecular Biology 35: 35-47.

  • Brar DS and Khush GS. 2002. Transferring genes from wild species into rice. In: Quantitative Genetics, Genomics and Plant Breeding, editor Kang MS. CAB International, Wallingford, UK, pp 197-217.

  • Brar DS and Khush GS. 2006. Cytogenetic manipulation and germplasm enhancement of rice. In: Genetic Resources, Chromosome Engineering and Crop Improvement, eds. Singh RJ and Jauhar PP. CRC Pres, pp 115-58.

  • Cause M and Ghesquiere A 1991. Prospective use of Oryza longistaminata for rice breeding. Proceedings of the 2nd International Rice Genetics Symposium. IRRI, Los Banos, Phillipines, pp 81-89.

  • Cheema K, Grewal N, Vikal Y, Sharma R, Lore JS, Das A, Bhatia D, Mahajan R, Gupta V, Bharaj TS and Singh K. 2008. A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4 Land transferred to Oryza sativa L. Genetical Research 90: 397-407.

  • Chen LJ, Lee DS, Song ZP, Shu HS and Lu BR. 2004. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Annals of Botany 93: 67-73.

  • Debbarma D and Khanna VK. 2018. Intervarietal hybridization and genetic diversity of rice by molecular markers. Current Investigations in Agriculture and Current Research 3(4): 394-403.

  • Hayes, HK, Immer, FR and Smith DC 1955. Methods of Plant Breeding (2nd edition). Mcgraw Hill Book Co, New York.

  • Hittalmani S and Shivashanker G. 1987. Outcrossing on male sterile plants of rice. Mysore Journal of Agricultural Sciences 21: 158-160.

  • Hossain M. 1996. Rice-wheat production system in South Asia: recent developments and constraints to expansion. Presented at the International Crop Science Conference, New Delhi, India.

  • Jena KK and Khush GS. 1986. Production of monosomic alien addition lines of Oryza sativa having a single chromosome of O. officinalis. In: Rice Genetics I. International Rice Research Institute, Manila, Philippines, pp 119-208

  • Ji Zhi-juan, Yang Shu-dong, Zeng Yu-xiang, Liang Yan, Yang Chang-deng and Qian Qian. 2016. Pyramiding blast, bacterial blight and brown plant hopper resistance genes in rice restorer lines. Journal of Integrative Agriculture 15 (7): 1432-1440.

  • Kanya JI, Hause TP, Kinyamario JI and Amugune NO. 2012. Hybridization potential between cultivated rice Oryza sativa and African wild rice Oryza longistaminata. International Journal of Agricultural Research 7 (6): 291-302.

  • Khush GS. 1977. Disease and insect resistance in rice. Advances in Agronomy 29: 265-341.

  • Khush GS, Bacalangco E and Ogawa T. 1990. A new gene for resistance to bacterial blight from O. longistaminata. Rice Genetics Newsletter 7: 121-122.

  • Khush, GS. 2005. What it will take to feed 5 billion rice consumers in 2030. Plant Molecular Biology 59: 1-6.

  • Kou Y and Wang S. 2010. Broad-spectrum and durability: understanding of quantitative disease resistance. Current Opinion in Plant Biology 13: 181-185.

  • Langevin SA, Clay K and Grace JB. 1990. The incidence and effect of hybridization between cultivated rice and its related weed red rice (Oryza sativa L.). Evolution 44: 1000-1008.

  • Li Ting, Liu Bo, Spalding Martin H, Weeks Donald P and Yand B. 2012. High-efficiency TALEN-based gene editing produces disease-resistant rice. Nature Biotechnology 30: 390-392.

  • Mew TW. 1989. An overview of the world bacterial blight situation. In: Bacterial Blight of Rice. International Research Institute, Manila, Philippines, pp 7-12.

  • Mahadevappa M. 1973. A cent per cent male sterile hybrid in rice at RRS Mandya offers great scope for research on inducing desirable characters. Current Research 2: 91.

  • Mishra B, Viraktamath BC, Iyas Ahmed M, Ramesha MS and Vijayakumar CH. 2003. Hybrid rice research and development in India. Proceedings of the 4th International Symposium on hybrid rice, Hanoi, Vietnam Los Banos (Philippines), eds Virmani SS, Mao CX, and Hardy B. International Rice Research Institute, Manila, pp 265-283.

  • Niruntrayakul S, Rerkasem B and Jamjod S. 2009. Crossability between cultivated rice (Oryza sativa) and common wild rice (O. rufipogon) and characterization of F and F populations. Science Asia 35: 161-169. 1 2

  • Oka HI and Morishima H. 1967. Variations in the breeding systems of wild rice, Oryza perennis. Evolution 21: 249-258.

  • Ou SH. 1985. Rice Diseases (2nd edn.). Commonwealth Mycological Institute, Kew Surrey, UK, The Cambrian News Ltd., pp 109-201.

  • Rathour R, Katoch A, Kusum, Kaushik RPand Sharma TR. 2011. Virulence analysis of Magnaporthe oryzae for resistance gene deployment in north-western Himalayas. Plant DiseaseResearch 26(2): 183.

  • Ronald PC, Albano B, Tabien R, Abenes L, Wu KS, McCouch S and Tanksley SD. 1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Molecular and General Genomics 236: 113-20.

  • Sahadevan PC and Namboodiri KMN. 1963. Natural crossing in rice. Proceedings of the Indian Academy of Sciences SectionB58: 176-18.

  • Sakai KI and Narise T. 1959. Studies on the breeding behavior of wild rice. The Japanese Journal of Genetics 9: 64-65.

  • Salgotra RK, Gupta BB and Singh S. 2009. Evaluation of various floral traits in some rice CMS lines that influence seed setting under subtropical conditions. SABRAO Journal of Breeding and Genetics 41(2): 115-122.

  • Sanchez Paul L, Wing Rod A and Brar DS. 2014. The wild relatives of rice: genomes and genomics. In: Genetics and Genomics of Rice, Plant Genetics and Genomics: Crops and Models, eds Zhang and Wing RA.Springer, New York, pp 9-25.

  • Sano Y. 1989. The direction of pollen flow between two cooccuring rice species, Oryza sativa and O. glaberrima. Heredity 63: 353-357.

  • Sheeba A, Vivekanandan Pand Ibrahim SM. 2006. Genetic variability for floral traits influencing outcrossing in the CMS lines of rice. Indian Journal of Agricultural Research 40 (4): 272-276.

  • Shikari Asif Bashir, Rajashekara H , Khanna Apurva, Krishnan S. Gopala, Rathour R, Singh UD, Sharma TR, Prabhu KV and Singh AK. 2014. Identification and validation of rice blast resistance genes in Indian rice germplasm. Indian Journal of Genetics 74 (3): 286-299.

  • Sidharthan B, Thiyagarajan K and Manonmani S. 2007. Cytoplasmic male sterile lines for hybrid rice production. Journal of Applied Sciences Research 3 (10): 935-937.

  • Singh SP, Goel RK, Hunjan MS, Vikal Y and Lore JS. 2012. Screening of land races of rice (Oryza sativa) for bacterial blight resistance and marker assisted surveying of known Xa/xa gene(s). Plant Disease Research 27: 209-215.


Abstract Views: 230

PDF Views: 1




  • Crossability Among Backcross Progenies Harbouring Different Resistant Genes for Rice Blast and Bacterial Blight Diseases and Elite Genotype HPR 2143 of Rice

Abstract Views: 230  |  PDF Views: 1

Authors

Ruchi Chauhan
Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Rakesh Kumar Kapila
Department of Seed Science and Technology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India
Rajeev Rathour
Department of Agricultural Biotechnology, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur - 176 062, India

Abstract


Rice blast and bacterial leaf blight are the two major diseases affecting rice productivity in north-western Himalayan region of India. Exploitation and utilization of major resistance (R) genes is an effective way to control these diseases. The selected elite BC2F2 progenies of four crosses, HPR2143/DHMAS164, HPR2143/PB1, HPR2143/IRBB54 and HPR2143/PR114 were used as a source for blast resistance genes Pita and Pi9, and bacterial leaf blight resistance genes Xa21 and Xa38 respectively. In order to pyramid 2 genes for each disease, hybridization among these backcross derivatives containing single genes was attempted. Overall 346 spikelets were emasculated and pollinated to combine genes, Pita and Pi9, out of which 136 seeds were set with a mean crossability of 39.31 per cent. Similarly to combine genes, Xa21 and Xa38, 335 spikelets were emasculated and pollinated in which 127 seeds were set with an overall crossability of 37.91 per cent. Overall crossability over both sets of crosses (Pita × Pi9) and (Xa21 × Xa38) was recorded to be 38.61 per cent that was comparatively low as compared to earlier documented reports.

Keywords


Rice, Hybridization, Pita, Pi9, Xa21, Xa38.

References